Agilent Technologies 1260 Infinity II, Руководство пользователя

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Agilent 1260 Infinity II Preparative Autosampler User Manual 13
Introduction
1
Operating Principle
Operating Principle
Sampling Sequence
The preparative autosampler processor continuously monitors the movements
of the preparative autosampler components during the sampling sequence.
The processor defines specific time windows and mechanical ranges for each
movement. If a specific step of the sampling sequence can’t be completed
successfully, an error message is generated.
During the sampling sequence, the solvent bypasses the preparative sampler
via the injection valve. The gripper arm selects the sample vial, either from a
static sample rack, or from external vial positions. The gripper arm places the
sample vial below the injection needle. The required volume of sample is
drawn into the sample loop by the metering device. Sample is applied to the
column when the injection valve returns to the mainpass position at the end of
the sampling sequence.
The sampling sequence occurs in the following order:
1
The injection valve switches to the bypass position.
2 The piston of the metering device moves to the initialization position.
3
The gripper arm moves from the home position, and selects the vial. At the
same time, the needle lifts out of the seat.
4 The gripper moves into the needle station and stops in the draw position.
1
5
The needle lowers into the vial.
6
The metering device draws the defined sample volume.
7 The needle lifts out of the vial.
8 The gripper arm moves out slightly and stops in the wash position
1
.
9
The needle moves downwards and dips into the wash well of the wash port.
Simultaneously the peristaltic pump delivers the flush solvent.
1
10
The needle moves back.
1
1
only if automated needle wash is selected. If this feature is disabled, the gripper arm positions the
sample vial directly below the needle (Step 4) and lowers the needle into the vial.