Table C–2: QSM-R specifications (continued)
Attribute
Specification
Gradient formation
Low-pressure mixing, quaternary gradient
Gradient profiles
11 gradient curves [including linear, step (2), concave (4),
and convex (4)]
Check valves
Passive check valves
Flow accuracy
+/- 1.0% of set flow at 0.5, 3.0, and 5.0 mL/min
[Degassed methanol, gravimetric or volumetric, with a back
pressure of ≤6895 kPa (≤69 bar, ≤1000 psig]
Flow precision
0.075% RSD or +/-0.02 min SD, whichever is greater based
on 6 replicates
[60:40 water/methanol pre-mixed; 1.5 mL/min;
alkylphenone mix; 24.0 µL injection volume; CORTECS C
18
2.7 µm, 4.6 × 50 mm; 35 °C; HPLC @ 254 nm]
Composition ripple
(baseline noise)
<0.5 mAU average peak-to-peak with appropriately sized
filter or mixer
[mobile phase containing 0.1% TFA in water/acetonitrile; 1.5
mL/min; CORTECS C
18
2.7 µm, 4.6 x 50 mm; 30 °C; UV @
214 nm]
Composition
accuracy
+/- 0.5% absolute (full scale) from 5 to 95%; 0.5 to 5.0
mL/min
[methanol; methanol with 5.0 mg/mL caffeine step gradient;
UV @ 273 nm]
Composition
precision
0.15% RSD or +/- 0.04 min SD, whichever is greater based
on six replicate injections
[60:40 water/methanol using the Auto•Blend Plus
technology; 1.5 mL/min; alkylphenone mix; 24.0 µL
injection volume; CORTECS C
18
2.7 µm, 4.6 × 50 mm; 25
°C; UV @ 254 nm]
Compressibility
compensation
Automatic and continuous
Pump seal wash
Standard
Primary wetted
materials
316 stainless steel, DLC, fluoropolymer, PEEK, PEEK blend,
PPS, ruby, sapphire, titanium alloy, UHMWPE blend, zirconia,
DLC
June 12, 2015, 715004747 Rev. A
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