Waters 2475, Operator'S Manual, Page: 93 / 228

Preparing to start a run 3-21
the minus symbol (–) produces an inverted chromatogram at the analog
output channel.
• Auto Zero-On-Inject – Selected by default, this parameter specifies the
auto-zero behavior each time the detector receives an inject-start signal.
You can disable this parameter by pressing any numerical key to clear
this field for either or both channels.
• Auto Zero on
λ
and gain changes – This function produces an auto zero
each time a wavelength change or gain change is requested. If you
disable it, significant changes in measured fluorescence can occur after
each wavelength change. Selecting “to zero” sets the signal level to zero.
Selecting “to baseline” maintains the previous baseline level when the
gain or wavelength change is made. “To baseline” is the default setting.
• Enable keypad & event-in chart mark – Selected by default, this
parameter results in a chart mark each time one is requested. You can
disable this parameter by pressing any numerical key, clearing this field
for either or both channels. Chart marks work with analog channels
only.
Primary and secondary function (method) parameters
Function Screen Type Units Range Default
x
λ
(Excitation
wavelength)
home Numeric nm Integer 200 to
890 nm
350 nm
e
λ
(Emission
wavelength)
home Numeric nm Integer 210 to
900 nm
397 nm
Tip:
The emission λ setting must always be at least 10 nm above the
excitation
λ
setting.
Gain 1 Numeric Emission
or
energy
units
0 to 1,000 0
EUFS 1 Numeric EUFS 1 to 100,000 10,000
Filter type 2 (of 4) Choice None • Hamming
• RC
• None Hamming