Waters 2475, Operator'S Manual, Page: 211 / 228

Wavelength selection C-11
Mixed mobile phases
The following table contains approximate wavelength cutoffs for some other
solvents, buffers, detergents, and mobile phases. The solvent concentrations
represented are those most commonly used. If you want to use a different
concentration, you can determine approximate fluorescence using Beer’s law,
because fluorescence is proportional to concentration.
Wavelength cutoffs for different mobile phases
Mobile phase
UV Cutoff
(nm)
Mobile phase
UV Cutoff
(nm)
Acetic acid, 1% 230 Sodium chloride, 1 M 207
Ammonium acetate,
10 mM
205 Sodium citrate, 10 mM 225
Ammonium bicarbonate,
10 mM
190 Sodium dodecyl sulfate 190
BRIJ 35, 0.1% 190 Sodium formate, 10
mM
200
CHAPS, 0.1% 215 Triethyl amine, 1% 235
Diammonium phosphate,
50 mM
205 Trifluoracetic acid,
0.1%
190
EDTA, disodium, 1 mM 190 TRIS HCl, 20 mM, pH
7.0,
pH 8.0
202, 212
HEPES, 10 mM, pH 7.6 225 Triton-X™ 100, 0.1% 240
Hydrochloric acid, 0.1% 190
Waters PIC
®
Reagent
A, 1 vial/liter
200
MES, 10 mM, pH 6.0 215 Waters PIC Reagent
B-6, 1 vial/liter
225
Potassium phosphate,
monobasic, 10 mM
dibasic, 10 mM
190
190
Waters PIC Reagent
B-6, low UV, 1 vial/liter
190
Sodium acetate, 10 mM 205 Waters PIC Reagent
D-4, 1 vial/liter
190