Waters 2475, Operator'S Manual, Page: 107 / 228

Operating the detector 3-35
Manual wavelength calibration
You can calibrate the detector manually from the keypad by pressing the
manual calibration key at any time during detector operation or when
calibration errors arise during startup. You need not restart the detector after
a successful wavelength calibration.
Tip:
Before you pump solvent or mobile phase through the system, flush the
lines with filtered, degassed, and sparged HPLC-grade water and continue to
pump at 1 mL/min.
To manually calibrate the wavelength:
1. Select Calibrate (Shift, 3) from the keypad.
Wavelength calibration message
2. Ensure the flow cell is prepared, and then press Enter.
The detector cycles through the calibration procedure and momentarily
displays a series of initialization messages similar to those displayed at
startup. If calibration is successful, the detector beeps three times.
If the maximum error is greater than 2.0 nm, the detector displays the
maximum error of the farthest calibration shift from the previous
calibration.
Calibration successful message
3. Press Enter. A “Calibration complete” message appears momentarily.
Other messages, such as “Optimizing system performance” and
“Restoring last setup”, can appear before the home screen reappears.