Waters 2475, Operator'S Manual, Page: 131 / 228

Scanning spectra 3-59
Programming a zero-scan
To program a zero-scan:
1. Select SCAN (Shift, Chart Mark).
2. Press 1 New Scan, then press 2 Zero Scan.
3. Press Next.
The second zero-scan parameter screen appears.
4. Specify the zero-scan parameters:
a. Select the type of zero-scan.
b. Specify the starting wavelength for the zero-scan, and then press
Enter.
c. Specify the ending wavelength for the zero-scan, and then press
Enter.
d. Specify the wavelength for the stationary monochromator, and then
press Enter.
e. Press Next.
5. Specify the Gain value, and then press Enter.
6. Specify the EUFS value, and then press Enter.
7. Specify a value in the Pace field for the rate at which the detector scans
the specified wavelength range.
Tip:
The default is 100 nm/min, and the allowable range is from 30 to
1000 nm. The figure “Scans of anthracene at 100 nm/min and 1000
nm/min” on page 3-53 shows two emission scans of anthracene, one at
100 nm/min and one at 1000 nm/min. The higher the number you enter
in the Pace field, the lower the resolution of the scan.
8. Press Next. The fourth zero-scan parameter screen appears.
9. Specify a number from 10 through 100 nm if you want to specify tick
marks, and then press Enter (see the figure “Scan of water without tick
marks” on page 3-54 and the figure “Scan of water with tick marks” on
page 3-54 ). Press CE if you want to disable tick marks.