Waters 2475, Operator'S Manual, Page: 136 / 228

3-64 Using the Detector
Three scans of anthracene in acetonitrile
Scanning using a static flow cell
To scan using a static flow cell:
1. Use a syringe to fill the flow cell with the mobile phase or solvent in
which your sample is dissolved.
2. Run a zero-scan (see page 3-57 ).
3. Use a syringe to fill the flow cell with the analyte.
4. Run a sample scan, keeping the pressure below 145 psi to avoid
overpressuring the flow cell.
5. Use the functions storage, review, subtract and review, and replay to
compare scanned data.
Sample emission scan 350
to 460 nm
Excitation=249 nm
Anthracene
Zoom of sample emission scan
350 to 440 nm
20 to 35 EU
Excitation=249 nm
Anthracene
λ
2 changed to 440 nm
Zoom of sample emission scan
360 to 420 nm
Excitation=249 nm
Anthracene
λ
1 changed to 360 nm
λ
2 changed to 420 nm
EU1 and EU2 on auto