78
Gentier Real-Time PCR System
Interface Introduction and Parameter Description
Reminding
: the parameter descriptions of
Sample Setting, Result Table
and
Heat Map
functional modules are same with those introduced in
D-3.3.4.1 Abs Quant
.
I.
Melting Curve
: display the fluorescence intensity against the temperature, as shown in figure
D-14.1.
Pre -melting
Range
Pos/ Neg
Threshold
Post- melting
Range
Figure D-14.1 HRM analysis interface - Melting Curve functional module
▶
Parameter introduction
:
X axis represents the temperature;
Y axis represents the Relative Fluorescence Unit (RFU);
By default, the application software will automatically determine normalized area of melting
curve. The two vertical sliders on the left are the
Pre-melting Range
threshold lines, which
are used to specify the pre-melting temperature range; while two vertical sliders on the right
are the
Post-melting Range
threshold lines, which are used to specify the post-melting
temperature range; the single line at the bottom is the
Pos/Neg Threshold
line, which is used
to determine the positivity or negativity of sample.
▶
Function key introduction
:
The introduction of
<
Y-axis Coordinate Adjustment >
and
<
Export >
keys are
same with those keys introduced in
D-3.3.4.1 Abs Quant - Amplification Curve
.
User can manually drag the vertical sliders or enter relevant parameter in the high resolution
melting analysis setting window to specify the normalized area of melting curve.
Summary of Contents for Gentier 48E
Page 14: ......
