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Gentier Real-Time PCR System
Reminding
: user can hover the mouse over a certain melting curve, the curve will be
highlighted and its corresponding channel and well coordinate will be displayed on the right
of cursor.
Reminding
: user can drag and drop the left mouse button to zoom in the local melting curve
diagram, or click the right mouse button to zoom out.
II.
Melting Peak
: display the first negative derivative of the fluorescence intensity (-dF/dT)
respect to the corresponding temperature, as shown in the figure 13.2.
If there is only a single
melting peak, it means that no non-specific PCR products are detected in the experiment.
Figure D-13.2 Melting Curve analysis interface - Melting Peak functional module
▶
Parameter introduction
:
X axis represents the temperature value;
Y axis represents the first negative derivative of the fluorescence intensity against the
corresponding temperature;
User can select the melting peak display mode from the drop-down list on the bottom left of
Melting Peak
functional module. The melting curve can be displayed according to
Well Color
,
Dye Color, Sample Color
and
Gene Color
.
The introduction of
<
Y-axis Coordinate Adjustment >
and
<
Export >
keys are
same with those keys introduced in
D-3.3.4.1 Abs Quant - Amplification Curve
.
<
Peak Area >
: user can click
<
Peak Area >
icon to define the melting peak area;
each side of the rectangle delimits the temperature and fluorescence range of the peak area.
Summary of Contents for Gentier 48E
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