73
Gentier Real-Time PCR System
b.
User can check a certain check box in
Remove
column and click
< OK >
to remove the
experiment data of samples with corresponding sample name from the relative quantification
analysis interface.
3.3.4.3 Melting Curve
For intercalating dyes (such as SYBR Green) and non-cleavable hybridization probes, the
fluorescence intensity is proportional to the amount of double-strand DNA (dsDNA). However,
these dsDNA including specific and non-specific PCR products, which means that the presence of
primer dimers and other non-specific products can affect the quality of real-time PCR data.
Melting curve show that the melting degree of dsDNA varies with the rising temperature. Different
dsDNA has different melting temperature T
m
(the temperature at which, 50% of the DNA has
dissociated from double-stramded to single-stranded) owing to different base sequence, fragment
length and GC content. Therefore, the melting curve is usually used to analyze whether there are
non-specific PCR products in PCR products.
Figure D-13. Melting Curve analysis interface
Summary of Contents for Gentier 48E
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