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Gentier Real-Time PCR System
▶
User can click
<
New Analysis >
icon in Tool Bar and the application software will
automatically pop up the new analysis window, as shown in figure D-8.
Figure D-8. New Analysis window
▶
The application software provides user with six analysis methods:
Abs Quant, Rel Quant,
Melting Curve, High Resolution Melting, Genotyping
and
End Point Fluorescence
.
Select Stage
: user can choose the stages that need to be analyzed.
Select Step
: user can choose the step that need to be analyzed.
Analysis Method
: shown the current anlysis method for the current step/stage.
User can choose the relevant data analysis method and click
< OK >
to confirm.
3.3.4.1 Abs Quant
The absolute quantification experiment is intended to quantify the amount of an interest nucleic
acid. Samples with unknown initial nucleic acid quantities are amplified as well as a dilution series
of gene-specific standard samples with known concentrations. The measured Ct values of the
standard samples are plotted against their known concentrations to obtain a regression line
named standard curve. The initial nucleic acid quantities of the samples can be obtained by
plotting their Ct values on the standard curve.
Summary of Contents for Gentier 48E
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