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View calibration images and transfer results to USB
The instrument performs the ROI, Uniformity, and Background calibrations in sequence. You can view
the calibration images after the Background calibration is complete.
1.
In the
Calibration Status
screen, touch
Details
.
2.
In the
Details
screen, touch a calibration type to view its images and plots.
Calibration
Example results indicating successful calibration
ROI
Note:
Select the desired filter
combination from the Filter Set
dropdown list.
Green circles around all wells and bright well centers.
Uniformity
Signals from each well following a uniform trend.
Background
Few, if any, signals with abnormally high fluorescence.
Dye
Signals from each well following a uniform trend, and each dye
peaks at the correct filter.
3.
In the
Calibration Status
screen, touch
Accept Results
or
Reject Results.
Accepting the results saves the calibration data to the instrument and overwrites existing data.
4.
(Optional)
Touch
Transfer EDS
to transfer the calibration data to a USB.
Chapter 5
Calibrate and verify instrument performance
Perform ROI/uniformity, background, and dye calibrations
5
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QuantStudio
™
1 Plus Real-Time PCR System Installation, Use, and Maintenance Guide