Observation
Possible cause
Recommended action
Forgot PIN for instrument profile
(continued)
Administrator forgot instrument
profile PIN.
Have another administrator reset the PIN for the
forgotten-PIN profile (see “Manage all instrument
profiles (Administrator only)”
).
If there is not another administrator profile on
the instrument, you must restore factory defaults
(see “Restore factory defaults”
).
High fluorescence signal
The reaction volume is not
correct.
Ensure that reaction volumes in the plate are
correct and match the volume that is entered in
the Method tab.
Signals that exceed the
limit of normal fluorescence
may indicate fluorescent
contaminants on the plate or the
sample block.
Examine the bottom of the reaction plate. If
there is contamination, prepare a new plate and
run the experiment again.
Identify the location of contamination on the
plate or sample block.
1. Obtain or prepare a background plate (see
2. Follow the procedures that are described in
Appendix B
Troubleshooting
Networking
B
QuantStudio
™
1 Plus Real-Time PCR System Installation, Use, and Maintenance Guide
89