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IMPORTANT!
Keep the bottom of the plate clean. Fluids and other contaminants on the bottom
of the plate can contaminate the sample block and cause an abnormally high background signal.
Perform calibrations
1.
In the home screen, select a calibration to perform.
Calibration
Touch
ROI/Uniformity
[1]
Settings
4
Maintenance and Service
4
Calibrations
4
ROI and Uniformity
Background
[2]
Settings
4
Maintenance and
Service
4
Calibrations
4
Custom
4
Background
Dye
Settings
4
Maintenance and Service
4
Calibrations
4
Dye
[1]
Automatically followed by Background calibration.
[2]
Initiate via this route if performing Background calibration only.
2.
Follow the instructions on the screen to start the calibration.
Note:
Dye calibration only
: Select the
Dye Plate
to run, then touch
Next
.
3.
Load the plate into the instrument.
4.
Touch
Start
.
5.
When the run is complete and the screen displays
Calibration Complete
, touch
View Results
to
check the calibration status.
Calibration status
Action
Passed
Touch
Next
to proceed to the next required calibration.
Failed
See “Troubleshoot calibration failure”
.
Note:
You can view the calibration images only after the ROI/Uniformity and Background
calibrations pass.
6.
Unload the plate from the instrument.
CAUTION!
PHYSICAL INJURY HAZARD.
During instrument operation, the plate temperature
can reach 100°C. Allow it to cool to room temperature before handling.
7.
Return the plate to its original packaging.
Note:
Each calibration plate can be used up to 3 times if the following conditions are met:
·
The plate is stored in its packing sleeve at –15 to –25°C.
·
The plate is used within 6 months after opening.
·
The plate is used before the plate expiry date
Chapter 5
Calibrate and verify instrument performance
Perform ROI/uniformity, background, and dye calibrations
5
QuantStudio
™
1 Plus Real-Time PCR System Installation, Use, and Maintenance Guide
57