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5.
Confirm that the liquid in each well is at the bottom of the well and free of bubbles. If it is not,
centrifuge the plate again.
IMPORTANT!
Keep the bottom of the plate clean. Fluids and other contaminants on the bottom
of the plate can contaminate the sample block and cause an abnormally high background signal.
Perform RNase P verification
1.
In the home screen, touch
Settings
4
Maintenance and Service
4
RNase P Verification
.
2.
Load the plate into the instrument.
3.
Touch
Start
.
4.
When the run is complete and the screen displays
Verification Complete
, touch
View Results
to
confirm the status of the run.
Calibration status
Action
Passed
Instrument is ready for use.
Failed
See “Troubleshoot verification failure”
5.
In the
RNase P Verification Status
screen, touch one of the following options:
•
Accept Results
— Save the results to the instrument.
•
Reject Results
— Delete the RNase P verification results.
•
Export Results
— Export the calibration results to a USB.
6.
Unload the plate from the instrument.
CAUTION!
PHYSICAL INJURY HAZARD.
During instrument operation, the plate temperature
can reach 100°C. Allow it to cool to room temperature before handling.
Chapter 5
Calibrate and verify instrument performance
Perform instrument verification using RNase P plates
5
QuantStudio
™
1 Plus Real-Time PCR System Installation, Use, and Maintenance Guide
63