27
Using the iBlot
®
Device with the De-Bubbling Roller,
Continued
Assembling the
iBlot
®
Device,
continued
4.
Clean the Metal Spacer 1 with a damp cloth or tissue and place the spacer
on the membrane as shown below.
5.
Place the prerun gel containing your protein samples on Metal Spacer 1
such that the gel is aligned to the lower right corner of the bottom stack
with the wells of the E-PAGE
™
gel facing up.
6.
Clean the Metal Spacer 2 with a damp cloth or tissue and place the spacer
over the gel as shown below.
7.
Remove the package labeled iBlot
®
Cathode Stack, Top from the iBlot
®
Gel
Transfer Stacks Box. Remove the iBlot
®
Cathode Stack, Top from the
package.
Spacer 1
Spacer 2
Summary of Contents for E-PAGE Gels
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