ThermoFisher Scientific E-PAGE Gels, Technical Manual

Page: 9 / 77

5
E-PAGE
™
System Components,
Continued
E-Base

Electrophoresis
Device
E-PAGE ™ Gels are used with a specially designed electrophoresis device that
combines a base and a power supply. Two types of devices are available from
Life Technologies:
•
The Mother E-Base ™ (Cat. no. EB-M03) has an electrical plug that can be
connected directly to an electrical outlet and is used for electrophoresis of
one E-PAGE ™ Gel. The Mother E-Base ™ has been tested for electrophoresis
with up to three Daughter E-Bases
™
connected at one time.
• The Daughter E-Base ™ (Cat. no. EB-D03) connects to the Mother E-Base ™ ,
and together they can be used for the independent electrophoresis of 2 or
more E-PAGE ™ Gels. The Daughter E-Base ™ does not have an electrical plug
and cannot be used without a Mother E-Base
™
.
E-PAGE ™ Gels are not compatible with the E-Gel ® 96 bases
(Cat nos. G7100-01/G7200-01) previously available from Life Technologies. The
older E-Gel ® 96 bases do not have the ‘E-Base ™ ’ inscription on the platform.
Mother E-Base ™
Each Mother E-Base
™
has a pwr/prg (power/program) button (right side) and a
time button (left side) on the lower-right side of the base. The lower-left side of
each Mother E-Base ™ contains a light LED and a digital display. The gel cassette
is inserted into the two electrode connections. The Mother E-Base ™ is connected
to an electrical outlet with the electrical plug.
The E-Base
™ EP program is set to 14 minutes default time. See page 15 to adjust
time as recommended.
Mother E-Base

(–)
(+)
Pwr/prg button
Time button
Digital
display
To electrical outlet
LED Light