28
Using the iBlot
®
Device with the De-Bubbling Roller,
Continued
Assembling the
iBlot
®
Device,
continued
8.
Insert the steel iBlot
®
E-PAGE
™
Tab in the plastic tray groove with the tab
teeth facing up (figure A). Gently press the iBlot
®
Cathode Stack over the
teeth to allow the teeth to penetrate into the copper electrode (figure B).
Remove the iBlot
®
Cathode Stack, Top from the red plastic tray using the
iBlot
®
E-PAGE
™
Tab (figure C).
A
B
C
9.
Place the iBlot
®
Cathode Stack, Top
without the tray
on top of Metal
Spacer 2 with the copper electrode side facing up (and agarose side facing
down). Ensure that all layers are aligned to the right to perform efficient
de-bubbling.
10.
Insert the De-bubbling Roller into the two grooves and lower the
roller to its lowest location while holding the pull tab. The resulting
assembly consists of the gel, and cathode and anode stacks placed
between two Metal Spacers 1 and 2 with the De-bubbling Roller on
top of the assembly as shown below.
Summary of Contents for E-PAGE Gels
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