53
Expected Results
Western Blotting
Results obtained using an E-PAGE
™
48
8% Gel that was blotted using the semi-dry
protocol described in this manual are shown below.
MagicMark
™
XP Western Protein Standard (5 µL) was loaded in all sample and
marker wells. The gel was electrophoresed for 23 minutes using standard
conditions. Proteins were transferred to a 0.45-µm nitrocellulose membrane using
the semi-dry blotting protocol described in the manual.
Detection was performed with WesternBreeze
®
Anti-Mouse Immunodetection Kit
using a 1:1000 dilution of Anti-Xpress
primary antibody from Life Technologies.
Both top and bottom rows are identical.
209 kDa
19 kDa
Summary of Contents for E-PAGE Gels
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