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Drying of E-PAGE
™
Gels
Gel Drying
Introduction
We recommend drying E-PAGE
™
Gels using passive air-drying methods such
as the Large Gel Drying Kit. Due to the thickness of E-PAGE
™
Gels, vacuum
drying is not recommended and may cause the gel to crack.
Materials Needed
You will need the following items for drying one E-PAGE
Gel. See page 71 for
ordering information.
•
Large Gel Drying Kit
•
Gel-Dry
™
Drying Solution (or prepare your own gel drying solution
containing 30% methanol and 5% glycerol)
•
Rotary shaker
•
Staining Tray
Gel Drying
Protocol
A brief gel drying protocol using the Large Gel Drying Kit is provided below.
For more details on this system, refer to the Large Gel Drying Kit manual
or contact Technical Support (page 72).
1.
At the end of all staining and destaining steps, wash the E-PAGE
™
Gel three
times for 2 minutes each time in deionized water (100 mL) on a rotary
shaker.
2.
Decant the water and add fresh Gel-Dry
™
Drying Solution (100 mL per
E-PAGE
™
gel).
3.
Equilibrate the gel in the Gel-Dry
™
Drying Solution by shaking the gel for
15–20 minutes in the staining container.
Note
: Do not equilibrate gels
stained with Coomassie R-250 in the Gel-Dry
™
Drying Solution for more
than 5 minutes to avoid losing band intensity.
4.
Cut any rough edges off the gel (including the wells and the gel foot) using
a gel knife.
5.
Remove 2 pieces (per gel) of cellophane from the package.
6.
Immerse one sheet at a time in the Gel-Dry
™
Drying Solution. Allow
10 seconds for complete wetting before adding additional sheets. Do not
soak the cellophane for more than 2 minutes.
Summary of Contents for E-PAGE Gels
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