66
Additional Staining Protocols,
Continued
Oligohistidine
Staining
Procedure,
Continued
7.
Place the gel on a UV transilluminator equipped with a standard camera and
select an appropriate filter on the camera (stain emission maxima is 572 nm).
You may also use a laser-based scanner with a laser line that falls within the
excitation maxima of the stain (532 nm).
8.
Image the gel with a suitable camera with the appropriate filters using a
1–4 second exposure.
Results
Results obtained using an E-PAGE
™
48
8%
Gel stained with Pro-Q
®
Sapphire
532 Oligohistidine Gel Stain are shown below.
The gel contains the following samples: (lanes not indicated are blank)
Lane
Sample
1
SeeBlue
®
Plus2 Pre-stained Standard (10 µL)
2, 3, 4, 5, 6, 7, 8, 9
Purified ovalbumin phosphoprotein, 35 kDa
(5, 10, 25, 50, 100, 250, 500, 1000 ng)
10, 11, 12, 13, 14, 15, 16, 17
Negative control, lysozyme
(5, 10, 25, 50, 100, 250, 500, 1000 ng)
1 2 3 4 5 6 7 8 9
10 11 12 13 14 15 16 17
Summary of Contents for E-PAGE Gels
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