39
Visualizing Lumio
Fusion Proteins
Introduction
The steps involved in detecting Lumio
™
fusion proteins run on an E-PAGE
™
Gel
are described below.
To visualize Lumio
™
fusion protein bands after electrophoresis, you will need a
UV transilluminator or a laser-based scanner (see below). For further details on
imaging Lumio
™
fusion proteins, refer to the product manual available at
or contact Technical Support (page 72)
Visualizing
Lumio
™
Fusion
Proteins
After electrophoresis is complete, immediately visualize and image the gel as
described below.
There is no need to remove the E-PAGE
™
Gel from the
cassette to visualize Lumio
™
fusion proteins.
1.
Place the gel cassette on a UV transilluminator equipped with a camera and
select the ethidium bromide or SYBR
®
Green filter on the camera.
You may also use a laser-based scanner with a laser line that falls within the
excitation maxima of the stain (500 nm), and a 535 nm long pass filter or a
band pass filter centered near the emission maxima of 535 nm.
Note:
Adjust the settings on the camera prior to turning on the UV
transilluminator. Avoid exposing the gel to UV light for long periods of time.
2.
Image the gel with a suitable camera with the appropriate filters using a 4–
10 second exposure. You may need to adjust the brightness and contrast to
reduce any faint non-specific bands.
You should see fluorescent bands of Lumio
™
fusion proteins and the gel should
have minimal background, as shown on page 54.
Summary of Contents for E-PAGE Gels
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