14
Electrophoresis of E-PAGE
™
Gels,
Continued
•
Always load 5–10 µL deionized water first into all wells prior to loading
sample or molecular weight standard.
•
E-PAGE
™
Gels can only be used once.
Do not re-use
.
•
To obtain the best results, run the E-PAGE
™
Gel immediately after removal
from the pouch and loading.
•
Store and run E-PAGE
™
Gels at room temperature.
•
For optimal results, we do not recommend running reduced and
non-reduced samples on the same gel. If you do choose to run these
samples on the same gel, avoid running reduced and non-reduced samples
in adjacent lanes as the reducing agent may have a carry-over effect on the
non-reduced samples.
•
Avoid running samples containing different salt or protein concentrations
in adjacent lanes.
•
E-PAGE
™
48
and
96
Gels are not compatible with the E-Gel
®
96
Mother
and Daughter Bases (Cat nos. G7100-01/G7200-01) available previously
from Life Technologies. The older E-Gel
®
96
bases do not have the “E-
Base
™
” inscription on the platform.
Method of
Loading Samples
We recommend the following methods of sample loading based on the gel type.
Use short, rigid tips for loading E-PAGE
™
Gels
.
Gel Type
Method of Loading
E-PAGE
™
48
Manual pipetter, multichannel pipetter (load samples
into alternate wells of the gel followed by a second round
of loading into the remaining wells), or automated liquid
handling devices (8- or 12-tip).
See page 17 for automated liquid handling system
guidelines.
E-PAGE
™
96
Manual pipetter, multichannel pipetter, or automated
liquid handling devices (8-, 12-, or 96-tip).
See page 17 for automated liquid handling system
guidelines.
Summary of Contents for E-PAGE Gels
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