20
Using E-Holder
™
Platform
Introduction
The E-Holder
™
Platform is designed to hold E-PAGE
™
Gels during loading. You
can use the E-Holder
™
Platform when you need to load multiple gels while other
gels are running on the E-Base
™
Note
: The E-Holder
™
Platform is not a power supply unit, cannot be connected
to an electrical outlet, and cannot be used to run E-PAGE
™
48
or
96
Gels.
To obtain the best results, run E-PAGE
™
Gels on the Mother E-Base
™
Device or
Daughter E-Base
™
Device within 15 minutes after loading on E-Holder
™
Platform.
The E-PAGE
Gels will not fit on the E-Gel
96
Holder previously available
from Life Technologies.
Using E-Holder
1.
Open the package and remove the E-PAGE
™
Gel.
2.
Remove comb from the E-PAGE
™
cassette.
3.
Place the E-PAGE
™
cassette in the E-Holder
™
Platform. Align the bottom-left
end of the cassette in the lower-left alignment corner of the E-Holder
™
Platform
as shown in the figure below. Proceed to loading the gel or to Step 4
below for automated liquid handling system loading.
4.
Optional
:
Set up your automated liquid handling system to load samples
into the E-PAGE
™
48
or
96
Gel placed on an E-Holder
™
Platform. Program
your automated liquid handling system to load the samples approximately
5 minutes before the previous electrophoresis run is complete. This ensures
that the loaded gel from the E-Holder
™
Platform is placed onto an E-Base
™
Device within the recommended time of 15 minutes.
E-PAGE
™
96
Gel
E-Holder
™
Platform
Alignment
corner
Summary of Contents for E-PAGE Gels
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