Thermo Scientific Surveyor MSQ Plus, Hardware Manual

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Thermo Scientific Surveyor MSQ Plus Hardware Manual 119
A
Optimizing the LC Conditions
This appendix includes helpful information for optimizing your LC system and your
Cone Wash system.
Flow Rates
In general, the LC column employed determines the choice of flow rate. Each column has an
optimum flow rate. The guidelines in
Table 8 apply.
The different ionization modes require different flow rates and column IDs. The following
guidelines apply when using the Surveyor MSQ Plus MS detector:
• Electrospray can operate at all the flow rates described in Table 8 .
• APCI cannot operate at flow rates below 0.2 mL/min. Therefore, suitable column IDs are
2.1 mm, 3.9 mm and 4.6 mm.
Contents
• Flow Rates
• LC Solvents and Mobile Phase Additives
• Cone Wash System
• Flow Splitting
• PEEK Tubing
Table 8. LC columns and flow rates
Column ID Flow Rate
4.6 mm 1.0 mL/min
3.9 mm 0.5 mL/min
2.1 mm 0.2 mL/min
1.0 mm 40-50
μ
L/min
Capillary <10
μ
L/min