Thermo Scientific Orion Star
™
and Star Plus Meter User Guide
EN-46
ISE Calibration
The calibration standards should be prepared in the same ISE units as the
desired sample results . Start the calibration with the lowest concentration
calibration standard and work up to the highest concentration calibration standard .
Any reagents, such as ionic strength adjustors, should be added to samples
and standards as specified in the electrode user guide .
1 . Prepare the electrode, standards and any other required solutions for use
according to the electrode user guide .
2 . In the measurement mode, press
until the arrow icon points to the top line,
press
until the
ISE
icon is shown and press
to begin the calibration .
3 . Rinse the electrode with distilled or deionized water, shake any excess water off,
blot it dry and place the electrode into the least concentrated standard .
4 . Wait for
ISE
icon to stop flashing . Press
until the first digit to be changed
is flashing, press
/
to change the value of the flashing digit and
continue to change the digits until the meter displays the concentration value
of the standard . Once the standard value is set, press
until the decimal
point is in the correct location .
5 . Press
to proceed to the next lowest calibration standard and repeat
steps 3 and 4, working from the lowest concentration standard to the highest
concentration standard, or press
to save and end the calibration .
6 . The actual electrode slope, in mV per decade concentration, will be displayed
in the main field and
will be displayed in the lower field .
a . For a one point calibration, press
and
/
to edit the slope .
To change the sign of the slope to negative or positive, press
until
none of the digits are blinking and the arrow icon is blinking and press
to change the sign of the slope . Press
to return to the measurement
mode .
b . For a two or more point calibration, the meter will automatically proceed
to the measurement mode after the slope is displayed .
ISE Technique
Summary of Contents for Orion Star
Page 367: ......
