Thermo Scientific NanoDrop OneC, User Manual

Page: 74 / 144

4
Learning Center
Prepare Samples and Blanks
70 NanoDrop One
C
with NanoDropQC Software User Guide Thermo Scientific
1. From the Home screen, select an
application name.
2. Lift the instrument arm and clean the
upper and lower pedestals with new
laboratory wipe.
3. Measure a water blank:
– Pipette exactly 1 µL deionized water
(DI H
2
O) onto the lower pedestal and
lower the arm.
– Tap Blank and wait for the
measurement to complete.
– Lift the arm and clean both pedestals
with new laboratory wipe.
4. Measure the buffer solution:
– Pipette 1-2 µL buffer solution onto the
pedestal and lower the arm.
– Start the sample measurement:
– if Auto-Measure is On, lower arm
– if Auto-Measure is off, lower arm
and tap
Measure
– Wait for measurement to complete.
The resulting spectrum should vary no
more than 0.04 A from the baseline at the
analysis wavelength.
If your spectrum does not meet these
criteria, repeat steps 2–4.
If spectrum is still outside specifications,
see
Solutions for Blanking Problems .
5. When you are finished with the blanking
cycle, tap End Experiment .
6. Lift the arm and clean both pedestals with
a new wipe.
Example spectrum of buffer suitable
for Protein A280 protein quantification
Example spectrum of buffer unsuitable
for Protein A280 protein quantification