3
Applications
Measure UV-Vis
12
NanoDrop One
C
with NanoDropQC Software User Guide
Thermo Scientific
Measure UV-Vis
Measure UV-Vis
The UV-Vis application allows the instrument to function as a conventional
spectrophotometer. Sample absorbance is displayed on the screen from 190 nm to 850 nm.
Up to 40 wavelengths can be designated for absorbance monitoring and inclusion in the
report. Automatic pathlength adjustment and a single-point baseline correction can also be
used.
To make UV-Vis measurements
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the instrument
arm and clean the upper and lower pedestals. At a minimum, wipe the pedestals with a new
laboratory wipe. For more information, see
.
Measures the absorbance of any sample at
up to 40 wavelengths across the ultra-violet
(UV) and visible regions of the spectrum.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into
the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will permanently
damage the quartz fiber optic cables.
To measure a sample using the UV-Vis application
1. From the Home screen, select
UV-Vis
.
2. Specify up to
(or you can specify them later if desired) and
whether automated pathlength adjustment, analysis wavelength, and baseline correction
will be used.
