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3

Applications

Measure UV-Vis

Thermo Scientific

NanoDrop One

C

with NanoDropQC Software User Guide

17

Settings for UV-Vis Measurements

To show the UV-Vis settings, from the Home screen, select

UV-Vis

.

Setting

Available Options

Description

Monitored
wavelengths

Enter up to 40
wavelengths between
190 nm and 850 nm

User-defined wavelengths to be measured and reported at run
time

. Absorbance values for the first three entered wavelengths

are displayed in the

screen. To see absorbance

values for 8 monitored wavelengths, swipe left in the
measurement screen to show the

. To see all monitored

wavelengths, press and hold a sample row to show the

screen (scroll up to display absorbance values for any

additional user-defined wavelengths).

Note

: If Baseline Correction is selected, all displayed absorbance

values are the corrected values.

Analytical Wavelength

Any wavelength between
190 nm and 850 nm

This is the wavelength the software will use to determine the
pathlength selection.

Automated Pathlength On or Off

(affects pedestal
measurements only)

Optional automated pathlength selection

. Allows the software

to use the optimal (shorter) pedestal pathlength for high
concentration samples to help prevent detector saturation (see

Detection Limits

• When selected, the shorter pathlength is used when any

wavelength between 220 nm and 850 nm has 10 mm
equivalent absorbance value of 12.5 or higher. For
wavelengths between 190 nm and 219 nm the change to the
shorter pathlength occurs when any wavelength in this range
has a 10 mm equivalent absorbance value of 10 or higher.

• When deselected, the pedestal pathlength is restricted to

10 mm across all wavelengths.

Note

: In either case, displayed absorbance values have been

normalized to a 10 mm pathlength equivalent.

Baseline Correction

On or off

Enter baseline correction
wavelength in nm or use
default value (750 nm)

Optional user-defined baseline correction

. Can be used to

correct for any offset caused by light scattering particulates by
subtracting measured absorbance at specified baseline correction
wavelength from absorbance values at all wavelengths in sample
spectrum. As a result, absorbance of sample spectrum is zero at
specified baseline correction wavelength.

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Summary of Contents for NanoDrop OneC

Page 1: ...NanoDrop Micro UV Vis Spectrophotometer NanoDrop OneC with NanoDrop QC Software User Guide 269 342200 NanoDrop QC UG Revision A December 2019 ...

Page 2: ...ation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document su...

Page 3: ...r Instrument 7 Update Software 7 Setting Up User Account Control 8 User Account Control 8 Security Administration Policies 9 Technical Support 10 Chapter 3 Applications 11 Measure UV Vis 12 Measure UV Vis 12 To make UV Vis measurements 12 Best practices for UV Vis measurements 13 UV Vis Reported Results 14 Settings for UV Vis Measurements 17 Measure Chemometrics 18 Measure Chemometrics 18 Create C...

Page 4: ...oDrop One Measurement Screens 74 NanoDrop One General Operations 90 Instrument Settings 100 Data Deletion Settings 105 PC Control Software 107 PC Control Home Screen overview 107 Control options 108 History 108 Settings 109 Chapter 5 Maintenance 111 Maintenance Schedule 112 Cleaning the Touchscreen 113 Maintaining the Pedestals 114 Cleaning the Pedestals 114 Reconditioning the Pedestals 116 Decont...

Page 5: ... preloaded software and a touchscreen display NanoDrop QC PC Control software can be installed on a local PC and used to control the instrument and view data The instrument can be connected to an optional printer with a USB cable or to a remote printer through an Ethernet connection or wireless network NOTICE Locate the instrument away from air vents and exhaust fans to minimize evaporation NOTICE...

Page 6: ...le retention system The NanoDrop OneC also features a cuvette holder for analyzing dilute samples using standard UV visible cuvettes Touchscreen TheNanoDrop OneC comes with a built in 7 inch high resolution touchscreen preloaded with easy to use instrument control software The touchscreen can slide left or right to accommodate personal preference and tilt forward or back for optimal viewing ...

Page 7: ... assays cell cultures and kinetic studies The cuvette system has these features extended lower detection limits 37 C heater option for temperature sensitive samples and analyses micro stirring option to ensure sample homogeneity and support kinetic studies For details see Measure a Sample using a Cuvette USB A port Two more USB A ports are located on instrument back panel Instrument light path Cuv...

Page 8: ...nce Verification Solution Liquid photometric standard used to check instrument performance For more information see Performance Verification Specially formulated conditioning compound that can be applied to the pedestals to restore them to a hydrophobic state required to achieve adequate surface tension for accurate sample measurements The kit includes conditioning compound and applicators For mor...

Page 9: ...rmo Scientific NanoDrop OneC with NanoDropQC Software User Guide 5 Instrument Detection Limits Measurement Location Pathlength mm Upper Detection Limit 10 mm Equivalent Absorbance Pedestal 1 0 12 5 0 2 62 5 0 1 150 0 05 300 0 03 550 Cuvette 10 1 5 5 3 2 7 5 1 15 ...

Page 10: ...1 About the Spectrophotometer 6 NanoDrop OneC with NanoDropQC Software User Guide Thermo Scientific This page is intentionally blank ...

Page 11: ...ware and release notes from our website Follow the steps to update or upgrade the software on your local instrument and or install or update the NanoDrop QC software on a personal computer PC An Internet connection is required to download software To install or update NanoDrop QC software on a PC 1 Insert the USB flash drive containing the installer software into an available USB port on your PC 2...

Page 12: ...ntrol Launch the Security Administration application and select NanoDrop QC from the directory on the left to reveal Access Control and System Policies Access control Access control is used to grant or deny individual users or groups access to protected features in the instrument application Add and remove users and groups to the access list and set access rights using the drop down for each entit...

Page 13: ...s for users and groups Launch the Security Administration application and select NanoDrop QC System Policies You can add delete or edit policy groups and enable or disable the group s users permission to delete data When you are finished select Save Changes will take effect the next time NanoDrop QC is launched Changes made to the Security Administration policies are applicable to the local PC onl...

Page 14: ...a only Fax 302 792 7155 E mail nanodrop thermofisher com Website www thermoscientific com nanodrop For International Support please contact Contact your local distributor For contact information go to http www nanodrop com Order aspx If you are experiencing an issue with your system refer to the troubleshooting information If the issue persists contact us If you are outside the U S A and Canada pl...

Page 15: ...hemometrics application allows you to use your unique chemometrics method The method is created using the NanoDrop QC PC Control software that is installed on your PC and then loaded into the instrument though a USB storage device See the NanoDrop QC PC Control software to learn about Chemometric method features that are supported The Custom application provides additional flexibility for the meth...

Page 16: ... lower pedestals At a minimum wipe the pedestals with a new laboratory wipe For more information see Cleaning the Pedestals Measures the absorbance of any sample at up to 40 wavelengths across the ultra violet UV and visible regions of the spectrum Measure UV Vis Reported Results Settings Detection Limits NOTICE Do not use a squirt or spray bottle on or near the instrument as liquids will flow int...

Page 17: ...se a 1 2 µL sample volume See Recommended Sample Volumes for more information 3 Pipette 1 2 µL blanking solution onto the lower pedestal and lower the arm or insert the blanking cuvette into the cuvette holder Tip If using a cuvette make sure to align the cuvette light path with the instrument light path 4 Tap Blank and wait for the measurement to complete Tip If Auto Blank is On the blank measure...

Page 18: ...an example as it appears on the local instrument display Tap row to select sample and update spectrum tap more rows to overlay up to five spectra Press and hold sample row to view measurement details Drag tab down up to see more less sample data Swipe screen left to view table with more measurement results Pinch and zoom to adjust axes double tap to reset Tap to end experiment and export data UV V...

Page 19: ...Here is an example Note Micro volume absorbance measurements and measurements taken with nonstandard cuvettes are normalized to a 10 0 mm pathlength equivalent Note Scroll up to display absorbance values for any additional user defined wavelengths Sample name tap to edit Sampling method Application Date time measured Automated pathlength setting Baseline correction absorbance Absorbance at 450 nm ...

Page 20: ...fit spectra to window Measuresample solution Select to toggle Auto Measure ON OFF Sample name select to edit Run blank Back to Home screen ends experiment Tips Click sample row to select sample and update spectrum Shift click multiple sample rows to overlay up to five spectra Click a sample and hover locations on spectra to view measurement values Sample rows Tips Auto Measure default is ON Auto B...

Page 21: ...ength On or Off affects pedestal measurements only Optional automated pathlength selection Allows the software to use the optimal shorter pedestal pathlength for high concentration samples to help prevent detector saturation see Detection Limits for details When selected the shorter pathlength is used when any wavelength between 220 nm and 850 nm has 10 mm equivalent absorbance value of 12 5 or hi...

Page 22: ...he method must also reside on the instrument This is the only way to run a chemometric method if your instrument is not connected to the computer with an Ethernet cable Make chemometric measurements Measure Chemometrics Create Chemometric Method Edit Chemometric Method Reported Results Settings Detection Limits Note The instrument arm can be up during cuvette measurements which allows you to add r...

Page 23: ...copy the method file to the root of a portable USB device such as a memory stick Method files have a qmethod filename extension 2 Connect the USB device to one of the USB ports on the instrument 3 From the Home screen select the Chemometric Application icon 4 Use the list box at the top of the screen to indicate the USB port used 5 Select Load Method A message box shows the NanoDrop One methods av...

Page 24: ...ometrics Method Selection screen is displayed If one or more chemometric methods exist in the currently selected Data Storage Location they will be listed in the Select Method box A description of the selected method appears in the Method Details box 2 Select a method select an existing method by selecting the method name in the Select Method box 3 Select Run Method 4 Follow the on screen instruct...

Page 25: ...metrics Method Selection screen is displayed If one or more chemometric methods exist in the currently selected Data Storage Location they will be listed in the method selection pane below the search feature Details of the selected method appears in the method details pane to the right 2 Select a method select an existing method by selecting the method name in the method selection pane 3 Select Fo...

Page 26: ...are However once the method is created it can be saved in the NanoDrop One database on the local instrument or in the NanoDrop QC database on the PC To create a new Chemometrics method From the NanoDrop QC software select the Chemometric Method icon From the Chemometric Method Management pane select Enter both a name and description for your chemometric method Select the quant method qnt fiile you...

Page 27: ...ftware To edit an existing chemometrics method From the NanoDrop QC software select the Chemometric Method icon From the Chemometric Method Management screen select the method you would like to edit from the list of loaded methods From the drop down menu select Edit Adjust the method settings as desired You can select the components to be displayed on the LC Select Save Note The method is saved in...

Page 28: ...ple Tap row to select sample and update spectrum tap more rows to overlay up to five spectra Press and hold sample row to view measurement details Drag tab down up to see more less sample data UV visible spectrum Menu of options tap to open Analyte concentrations Swipe screen left to view table with more measurement results Pinch and zoom to adjust axes double tap to reset Note Micro volume absorb...

Page 29: ... method reported values The initial screen that appears after each measurement see previous image shows a summary of the reported values To view all reported values press and hold the sample row Here is an example Sample name tap to edit Sampling method Method name Date time measured Analyte concentration Method details ...

Page 30: ... in the table shows the absorbance values at all user defined wavelengths at a given stage and time Scroll down to see measurement information that is out of view The image below highlights the available features Swipe screen right to return to Rate measurement screen Tap to end experiment and export data Analyte concentration Press and hold row to view measurement details Measurement number Sampl...

Page 31: ...ils for a measurement from the absorbance measurement screen or data table press and hold the measurement row Here is an example Sample name Sampling method Date time measured Component results User defined wavelengths Method details scroll up to view more Application used Delete this measurement Return to previous screen Printthis screen ...

Page 32: ...etrics methods are defined by the quant method used From the instrument Home screen select Chemometrics and select a mehtod from the list The method details are displayed Settings can be edited only from the NanoDrop QC software In the PC Control software you can select which components are displayed on the instrument and set significant figures for each component ...

Page 33: ...if your instrument is not connected to the computer with an Ethernet cable Load custom methods onto the instrument 1 Export the method from the personal computer and copy the method file to the root of a portable USB device such as a memory stick Method files have a method filename extension 2 Connect the USB device to one of the USB ports on the instrument 3 From the Home screen select Custom Met...

Page 34: ...inimum wipe the pedestals with a new laboratory wipe For more information see Cleaning the Pedestals To measure a sample using a custom method using the local instrument interface 1 Make sure the method resides in same location as the database where you want to store the measurement results see To Load a Custom Method for details 2 From the Home screen select Custom Method 3 In the Select Method b...

Page 35: ... Method Details box 4 Select Run Method 5 Follow the on screen instructions to measure a sample To measure a sample using a custom method using the PC software 1 Make sure the method resides in same location as the database where you want to store the measurement results see To Load a Custom Method for details 2 From the Home screen select Custom Method ...

Page 36: ...e method selection pane select to select the method to run Information about the selected method appears in the method details pane 4 Select 5 Follow the on screen instructions to measure a sample Delete Custom Method From Home screen select Custom Method In Select Method box select a method to delete Select ...

Page 37: ...ap row to select sample and update spectrum tap more rows to overlay up to five spectra Press and hold sample row to view measurement details Drag tab down up to see more less sample data UV visible spectrum Menu of options tap to open Analyte concentration Swipe screen left to view table with more measurement results Pinch and zoom to adjust axes double tap to reset Note Micro volume absorbance m...

Page 38: ...d reported values The initial screen that appears after each measurement see previous image shows a summary of the reported values To view all reported values press and hold the sample row Here is an example Sample name tap to edit Sampling method Method name Date time measured Analyte concentration Method details ...

Page 39: ...d in Manage Custom Methods screen select and choose one of the following Formula if your method will not have standards Standard Curve if your method will have standards in the setup window enter Method Name this name appears in the Custom Setup box on the instrument after the method has been transferred there enter detailed Description of method if desired specify how to calculate and report the ...

Page 40: ...lect Method box along with their type formula or standards and Description From the Custom Method Management screen select the method you would like to edit from the list of loaded methods From the drop down menu select Edit View and adjust the method settings as desired Select Save Note If appears at the bottom left of the screen instead of a green check mark icon the method is invalid because it...

Page 41: ...ed make sure your selected spectral range includes your specified baseline correction and or analysis correction wavelength For micro volume absorbance measurements and measurements taken with non standard other than 10 mm cuvettes the spectra are normalized to a 10 mm pathlength equivalent Analysis wavelength correction Use this option to specify absorbance correction at analysis wavelength only ...

Page 42: ...tion coefficient for 1 cm pathlength and use adjacent drop down list to select appropriate unit Equation below shows how extinction coefficient is used to calculate sample concentration c A b where c analyte concentration A absorbance in absorbance units A wavelength dependent molar absorptivity coefficient or extinction coefficient b pathlength in cm determined at measurement time then normalized...

Page 43: ... and the expected range of the unknown samples Sample analyte concentrations are not extrapolated beyond the concentration of the highest standard Select curve fit type Specify type of equation used to create standard curve from standard concentration values Available options Linear Draws the linear least squares line through all measured standards requires reference measurement and at least one s...

Page 44: ...le when sample absorbance at the analysis wavelength is less than or equal to 12 5 10 mm pathlength equivalent the optimal longer pathlength is used When sample absorbance is greater than 12 5 the optimal shorter pathlength is used Recommended for samples that are highly absorbing at the analysis wavelength This option may cause reduced sensitivity when the sample spectra have a large absorbance p...

Page 45: ...lue is reported in Data Table and Sample Details screens Unit Enter unit for reported result After a measurement the unit is reported in Data Table and Sample Details screens Edit Edit selected formula for current method Delete Delete selected formula from current method Formula rules Custom formulas can include the following operators and functions Path Returns sample pathlength in cm A nm Return...

Page 46: ...ustom Method If you want to run a custom method and store the measurement results on the instrument the method must also reside on the instrument see Load a Custom Method for details Export Custom Method Export a custom method in order to run it and store the measurement results on the NanoDrop OneC instrument from the Custom Methods screen select a custom method from the kebab menu choose Export ...

Page 47: ...t method file choose Open imported method is added to end of Select Method list Edit custom method Edit a custom method in order to change the method settings from Custom Methods screen select a custom method from the list of available methods from the kebab menu choose Edit edit method settings as desired choose Save Delete custom method from Custom Methods screen select a custom method from the ...

Page 48: ...3 Applications 44 NanoDrop OneC with NanoDropQC Software User Guide Thermo Scientific This page is intentionally blank ...

Page 49: ...45 4 Learning Center Contents Micro Volume Sampling How it Works 46 Set Up the Instrument 48 Measure a Micro Volume Sample 58 Measure a Sample Using a Cuvette 63 Prepare Samples and Blanks 66 Basic Instrument Operations 71 Instrument Settings 100 PC Control Software 107 ...

Page 50: ...pectrophotometer uses surface tension to hold a small volume of sample between two pedestals The patented sample retention system enables the measurement of highly concentrated samples without the need for dilutions A fiber optic cable embedded in the upper pedestal leads to a xenon light source A second cable embedded in the lower pedestal leads to a detector When the instrument arm is down the s...

Page 51: ...layed range may vary for each application Sample Absorbance When the instrument is blanked a reference spectrum is taken of the blanking solution and stored in memory For each sample measurement the sample intensities along with the blank intensities are used to calculate the total absorbance of the sample according to the equation at the left Baseline Correction For some applications the instrume...

Page 52: ...ouse to the instrument use any USB port on the instrument front back left or back right See Accessories for information about accessories compatible with the NanoDrop One instruments Set Up Bluetooth Connections Use Bluetooth to connect the instrument to one or more Bluetooth wireless input devices such as a Bluetooth keyboard mouse or barcode scanner Ethernet Power on off Power USB A 2 CAUTION Av...

Page 53: ...s blue changes to On and software automatically searches for any available Bluetooth input devices If no Bluetooth devices are found after a few seconds the message No nearby Bluetooth devices were found is displayed to add a Bluetooth device follow manufacturer instructions to pair the device for example you may need to hold down a button and tap Search For Devices on instrument device name shoul...

Page 54: ...instructions to pair the device tap Back Bluetooth status is displayed at right of Bluetooth button repeat steps above to add another Bluetooth device or tap Done to close Settings Note If your Bluetooth device does not pair restart the device and then repeat the steps above to pair it with the instrument you may also try turning Bluetooth off and back on After a device is paired it remains paired...

Page 55: ...ps to select the devices to use or to deselect devices you don t want to use from instrument Home screen tap tap System tab tap Bluetooth to deselect a paired Bluetooth device such as a keyboard for input tap its Profiles button deselect Use For Input by clearing it s associated checkbox tap Paired Bluetooth Device in upper left to return to previous screen tap Back to return to System settings ta...

Page 56: ...Disconnect Bluetooth device from instrument Home screen tap tap System tab tap Bluetooth to disconnect paired Bluetooth device tap its Profiles button tap Unpair device is no longer listed under Paired Devices but remains in Available Devices list tap Back to return to System settings tap Done to close Settings ...

Page 57: ...ndard straight through Ethernet cable CAT5e or newer is recommended Set up Ethernet connection from instrument Home screen tap tap Networking tab tap Ethernet select an Ethernet option and choose OK Direct connection to a PC Select if you plan to connect an Ethernet cable between the NanoDrop OneC instrument and a personal computer Connection to a network jack Select if you plan to connect an Ethe...

Page 58: ... computer Ethernet port or an active network wall jack Set up Wireless Connections Select Wi Fi network on the instrument from instrument Home screen tap Settings tap Networking tab tap Wi Fi if Wi Fi is disabled button in upper right is set to OFF and no wireless networks are listed tap button to enable Wi Fi and display available Wi Fi networks Ethernet port ...

Page 59: ...nnection is successful the instrument is assigned an IP Internet Protocol address which appears at the right of the Wi Fi button as in the example below tap Done to exit Settings Note Some Wi Fi networks may require an identity password or other information before you can connect to them or they may be anonymous that is you may have to search for them by name For more information see the system ad...

Page 60: ...t of the instrument Home screen to quickly assess the instrument s connectivity status including Bluetooth and Wi Fi Show connectivity status tap on instrument Home screen to open System Status box tap OK to exit System Status Tap to show connectivity status Location of database where instrument is currently storing data Local instrument or Connected PC Wi Fi status Bluetooth status ...

Page 61: ...ronment meets these specifications operating temperatures 5 C 35 C 41 F 95 F relative humidity non condensing 20 80 Locate the instrument away from air vents and exhaust fans to minimize evaporation After the instrument is installed you can leave it turned on Note If operating the instrument at the low end of the recommended humidity range use adequate sample volume to avoid evaporation ...

Page 62: ...nted sample retention system enables the measurement of highly concentrated samples without the need for dilutions Tap here for details Supplies needed NanoDrop One or NanoDrop OneC spectrophotometer lint free laboratory wipes calibrated precision pipettor 0 2 µL sample material resuspended in appropriate buffer solution see Preparing Samples pure buffer solution for blanking instrument see Choosi...

Page 63: ...destals with DI H2O see Clean pedestals between users Recondition pedestals periodically to maintain their hydrophobic property Pipetting Samples Use recommended sample volumes to ensure proper liquid column formation Use calibrated precision pipettor 0 2 µL volume range with well fitting low retention precision tips to apply sample material to instrument for measurement If using low accuracy 0 10...

Page 64: ...n or near the instrument as liquids will flow into the instrument and may cause permanent damage Do not use hydrofluoric acid HF on the pedestals Fluoride ions will permanently damage the quartz fiber optic cables 1 From the instrument Home screen select an application UV Vis Chemometric or Custom Methods 2 Lift the instrument arm and clean the upper and lower pedestals with new laboratory wipe ...

Page 65: ...onto the pedestal and quickly lower the arm see Recommended Sample Volumes for more information Start the sample measurement if Auto Measure is On lower arm if Auto Measure is off lower arm and tap Measure When the sample measurement is completed the spectra and reported values are displayed 5 To measure another sample Lift the arm Clean both pedestals with new wipe Load the next sample and quickl...

Page 66: ...th pedestals with a new wipe If finished with the instrument for the day clean the pedestals with DI H2O see Clean pedestals between users Acquired data are automatically saved in an experiment with the entered name In the default configuration experiments are stored in a database on the local instrument according to acquisition date experiment name application used and any assigned labels see Man...

Page 67: ...tric assays cell cultures and kinetic studies The cuvette system offers an extended lower detection limit and an optional 37 C heater and micro stirrer Supplies needed NanoDrop OneC spectrophotometer lint free laboratory wipes two compatible cuvettes sample material resuspended in appropriate buffer solution see Preparing Samples pure buffer solution for blanking instrument see Choosing and Measur...

Page 68: ...tes to measure samples with analysis wavelengths in the UV range 340 nm Micro semi micro and ultra micro cuvettes should be masked Fill cuvettes with enough blanking or sample solution to cover instrument optical path 2 mm sample beam is 8 5 mm above cuvette bottom Lift instrument arm and make sure cuvette holder is free of debris When inserting quartz or masked plastic cuvettes align cuvette ligh...

Page 69: ...nufacturer for specifications Set stirrer and heater if desired Select Done See General settings for details 3 From the Home screen select an application 4 Measure a blank Fill clean dry cuvette with enough blanking solution to cover instrument optical path Lift instrument arm and insert blanking cuvette into cuvette holder making sure to align light path of cuvette with light path of instrument T...

Page 70: ...ulate sample concentration Ensure final analyte concentration is within instrument s absorbance detection limits For micro volume measurements gently but thoroughly vortex each sample before taking a measurement Avoid introducing bubbles when mixing and pipetting For more information watch multimedia training Effects of Bubbles in Samples 5 Measure a sample Fill clean cuvette to same height with s...

Page 71: ...s see To measure samples in the application used Measure new blank before each set of samples It is not necessary to blank the instrument before each sample measurement unless the samples are dissolved in different buffer solutions Measure a new blank every 30 minutes Run a blanking cycle to assess the suitability of your blanking solution before using it to perform sample measurements For a quick...

Page 72: ...ct Sample was inadvertently used to blank instrument Resulting sample spectra may exhibit negative absorbance values or in some cases resemble a mirror image of a typical pure nucleic acid or protein spectrum as in example at right Protein sample solution used to blank instrument results in mirror image spectrum Solutions for blanking problems Thoroughly clean and or recondition both pedestals and...

Page 73: ... solution you plan to use for sample analyses Supplies needed lint free laboratory wipes calibrated precision pipettor 0 2 µL buffer solution for evaluation To run a blanking cycle For quick demonstration watch multimedia training Evaluating a Blanking Solution for Suitability NOTICE Do not use a squirt or spray bottle on or near the instrument as liquids will flow into the instrument and may caus...

Page 74: ...1 2 µL buffer solution onto the pedestal and lower the arm Start the sample measurement if Auto Measure is On lower arm if Auto Measure is off lower arm and tap Measure Wait for measurement to complete The resulting spectrum should vary no more than 0 04 A from the baseline at the analysis wavelength If your spectrum does not meet these criteria repeat steps 2 4 If spectrum is still outside specif...

Page 75: ...de 71 Basic Instrument Operations NanoDrop One Home Screen NanoDrop One Measurement Screens Open Data Viewer NanoDrop One General Operations NanoDrop One Home Screen These operations are available from the NanoDrop One Home screen Data Viewer Instrument Settings Instrument Diagnostics System Status Applications ...

Page 76: ... example The available information is described below Instrument type Instrument model NanoDrop OneC Serial number Instrument serial number Instrument status Current status of the instrument Data storage location Indicates location of database set where instrument is currently storing data These options are available Local instrument Connected PC personal computer connected through Ethernet cable ...

Page 77: ...ent settings Instrument Diagnostics Tap on the Home screen to verify instrument operation Instrument diagnostics should be run periodically according to the recommended maintenance schedule See Instrument Diagnostics on page 122 for information about how to run the available instrument diagnostics Bluetooth status Status of Bluetooth connections for the instrument Connected to Enabled list of any ...

Page 78: ... experiment and export data Sample name tap to edit UV absorbance spectrum for selected sample Menu of options tap to open Measurement alert tap to learn more Tap to measure blank solution Page control swipe screen left to view table with more measurement results Tap to measure sample solution Auto Measure Measurement results see Applications for details Sample pathlength Home Return to NanoDrop O...

Page 79: ...nt as shown below In this example the first sample would be named Sample 1 followed by Sample 2 etc You can edit the default base name and overwrite any sample name Edit default sample base name After you measure a blank and before the first sample is measured tap Sample Name field to display keyboard enter new base name tap Done key Edit sample name from Home screen tap to open Data Viewer select...

Page 80: ... screens depend on the selected application For details see the reported results section of that application in this guide Applications application group Measure application name Reported Results Absorbance Spectrum For each measured sample each application shows the UV or UV visible absorbance spectrum and a summary of the results The vertical axis shows absorbance in absorbance units A The horiz...

Page 81: ...d before each group of similar samples The blank solution is typically the pure buffer that was used to resuspend the sample For more information see Choosing and Measuring a Blank Measure Button Tap Measure to measure a sample for the selected experiment Samples must be properly isolated and prepared before they can be measured with the instrument and the concentration must be within the instrume...

Page 82: ...e Measure or Blank operations for large batches of samples Auto Measure To select or deselect Auto Measure from any sample measurement screen tap the On or Off button at the right of the Measure button Auto Blank To select or deselect Auto Blank from any blank measurement screen tap the On or Off button at the right of the Blank button End Experiment Button Tap End Experiment when you are ready to...

Page 83: ...Operations Thermo Scientific NanoDrop OneC with NanoDropQC Software User Guide 79 After you tap End Experiment the End Experiment box is displayed Note The End Experiment button is enabled after the first sample measurement is completed ...

Page 84: ... local instrument front back left or back right or to a network location Export Button Allows you to select a file format for exporting the measurements in this experiment and then export the data to a USB device or network Available export file formats comma separated values spreadsheet csv file tab separated values spreadsheet tsv file spectral data only NanoDrop QC sql file The filename is the ...

Page 85: ...ent screen or data table to show the sample details which include all available measurement results and associated details for the selected sample Here is an example Information about the measured values displayed in Sample Details is provided in this Help system under the application used to acquire the data Note You can also edit the sample name from the Sample Details box ...

Page 86: ...ally saved in an experiment with an experiment name In the default configuration experiments are stored in the NanoDrop One database on the local instrument according to acquisition date experiment name application used and any assigned labels Use the Data Viewer to open the database on the local instrument in order to view acquired spectra and associated data from any experiment at any time Tap r...

Page 87: ... to open NanoDrop OneC database on instrument tap Data Viewer on instrument Home screen Menu Tap in the Data Viewer to see the available menu options Home Return to NanoDrop One Home screen Settings View or change instrument settings Import Import data from a USB flash drive Disk Status View remaining space available for storing measurement data on the instrument ...

Page 88: ...nge or other search filters The database is filtered using the current settings in the Search box Filters include time range application type and any user defined labels see Manage Identifiers for information about adding and deleting labels Here is an example Tap to change time range filter Tap to select or deselect application filters Tap to select or deselect user defined labels Change a filter...

Page 89: ...t in the Data Viewer to select experiments to be exported Export selected experiments open the Data Viewer and tap Select tap row to list experiments acquired on that date or use Search feature to find experiment tap to select one or more experiments to export tap again to deselect an experiment to select all experiments in database select All tap Export ...

Page 90: ...export to see Export Selected Experiments in General Operations for details and tap Export after Export Success message tap OK Delete Selected Experiments Use Select in the Data Viewer to select experiments to be deleted Delete selected experiments tap row in Data Viewer to list experiments acquired on that date or use Search feature to find desired experiment tap Select tap to select one or more ...

Page 91: ...to see the measurement data it contains Open an experiment tap row in Data Viewer to list experiments acquired on that date or use Search feature to find desired experiment tap experiment name to open the experiment Here is an example NOTICE Deleted data cannot be recovered One experiment measured on this date Tap to open this experiment press and hold to view or edit experiment details such as ex...

Page 92: ... below describes the available features Note The data shown are dependent upon the application used to measure the samples nucleic acids in these examples For more information see the application details Drag tab down up to see more less sample data Page control swipe screen left or right to view next or previous screen Pinch and zoom to adjust axes Tap row to select sample and update spectrum tap...

Page 93: ...ils Selected experiment Measurement alert tap to learn more Application used Page control swipe screen right to view previous screens 2 Menu of options tap to open Measurement results see Applications for details Tap to select unit Home Return to NanoDrop One Home screen Manage Identifiers Add or delete labels for selected experiment to make it easier to find see Manage identifiers on the instrume...

Page 94: ...e instrument or from the NanoDrop QC software installed on a personal computer see Manage Identifiers on a PC Use the Data Viewer to add labels to experiments assign existing labels view assigned labels and remove or delete labels on the instrument You can filter the list of experiments in the Data Viewer based on one or more user defined labels Label new experiment when you save it after the last...

Page 95: ...Find labeled experiments from Home screen tap to open Data Viewer tap Search in Search box select date range select application only applications that have associated data are shown select one or more identifiers from scrollable list and tap OK Remove a label from Home screen tap to open Data Viewer tap to open an experiment tap and choose Manage Identifiers in Manage Identifiers box select label ...

Page 96: ...nter a name for this group of measurements in the Experiment Name box tap End Experiment Edit experiment name from Data Viewer from Home screen tap to open Data Viewer tap row to list experiments acquired on that date or use Search feature to find experiment press and hold experiment name to open experiment details box ...

Page 97: ...iewer Measurement data can be exported in four formats as comma separated values csv files containing the measurement results and details for each exported experiment as tab separated values tsv files containing x y coordinates for every spectral data point for each exported experiment as NanoDrop QC sql files containing spectra and measurement results for each exported experiment Note Data export...

Page 98: ...es are the same as the experiment names The files are stored in a folder named NanodropOne followed by the instrument serial number Use System Status to view your instrument serial number Export data at end of experiment when finished measuring samples tap from End Experiment box set Export Data to an available export location front back left or back right USB port or a network location tap from E...

Page 99: ...5 Export data from Data Viewer from Home screen tap to open Data Viewer tap Select tap row to list experiments acquired on that date or use Search feature to find experiment tap to select one or more experiments to export tap again to deselect an experiment to select all experiments in database select All tap Export ...

Page 100: ...twork location and tap Export select one or more formats to export to see above for details and tap Export after Export Success message tap OK Delete Selected Measurements You can delete selected sample measurements from any experiment or all the measurements in the database Delete data from any measurement screen press and hold sample row to open Sample Details box tap NOTICE Deleted data cannot ...

Page 101: ...work Print data from any measurement screen after you have measured a sample display the measurement results to be printed such as the spectral data standard curve data table or sample details see NanoDrop One Measurement Screens if printing spectral data or the data table tap to select one or more sample rows to print tap again to deselect a sample row if no results are selected in data table all...

Page 102: ...ure to find desired experiment tap experiment name to open the experiment swipe left or right to select the type of data to print spectral data standard curve or data table tap to select one or more sample rows to print tap again to deselect a sample row if no results are selected in data table all results will be printed tap and choose Print choose OK to confirm in the Print Preview window make s...

Page 103: ...int Preview window make sure the correct printer is selected and set other print options as desired such as paper size and orientation Auto setting is recommended margin and alignment to adjust the image in the preview window choose Print If a label printer is connected to the instrument the software prints a label for the selected measurement If a full service printer is connected the selected sa...

Page 104: ...op One software and for any connected input device such as a keyboard mouse or barcode scanner Notice Changing the language requires a software restart Date and Time Automatic date time synchronize instrument date and time with available network Automatic time zone synchronize instrument time zone with available network Set date manually set instrument date this option is disabled when Automatic D...

Page 105: ...ails Version version of NanoDrop One operating software currently installed on this instrument Brightness Adjust brightness of instrument touchscreen Touch Sounds Provide audible feedback after each interaction with the touch pad Sound Volume Adjust volume of instrument touchscreen Activate Screen Saver Launch a screen saver application when the instrument has been idle for 30 minutes To reactivat...

Page 106: ...e this tab to specify one or more network paths for exporting acquired data when the instrument is connected to a network connection can be wired or wireless Network paths defined here will appear in the Export Data list box when exporting data from both the Data Viewer and the End Experiment box after you complete a measurement ...

Page 107: ...tered name will appear in the Export Data list box when exporting acquired data from the instrument Select Requires Authentication if the network path requires a user name and password Tap Save Location If the entered network path is valid its name is displayed in the Network Locations list on the Export Settings tab Edit Edit network path path name or authentication setting for selected network l...

Page 108: ...llowed by unique number starting with 1 Uses default Sample or user specified base name For details see Sample Name Use cuvette Select cuvette sampling mode available for NanoDrop OneC instrument model only When selected these additional options are available Pathlength Enter cuvette pathlength width before taking blank or sample measurements with cuvettes see cuvette manufacturer for cuvette spec...

Page 109: ...ange data deletion and set your password to secure deletion settings Enter your desired password and select Save Password You have the option to save a password reset key to a USB drive Saving a password reset key to a USB drive allows you to reset the password in the event you do not remember or have access to your password Data Deletion Settings Tap Change Settings to edit the data deletion sett...

Page 110: ...select Change Settings 2 Select Forgot Password 3 Implement password key If you have a USB with a password key insert the USB drive into the instrument and select Yes The password is reset and you will be now be able to enter a new password If you do not have a password key select No Continue to step 4 4 With a USB drive inserted into the instrument select Yes ...

Page 111: ... view data acquired with a NanoDrop One instrument on the PC as well as change instrument settings and create or edit custom and chemometrics methods PC Control Home Screen overview Figure 1 PC Control Home Screen Select your application from the icons just as you would with the NanoDrop OneC instrument Home Screen 5 Select Export This will generate a file for you to send to NanoDrop support so th...

Page 112: ...m the local PC History View data stored locally Filter by date or application Performance Performance verification process using PV 1 solution See Performance Verification on page 124 Intensity Run an intensity check for the cuvette or pedestal See Intensity Check on page 122 Pedestal Image Run a pedestal image check See Pedestal Image Check on page 130 Settings Set security server location and pa...

Page 113: ...oftware Thermo Scientific NanoDrop OneC with NanoDropQC Software User Guide 109 You can search history by name filter by date and filter by application Settings Use settings to modify security settings and view the selected database ...

Page 114: ...4 Learning Center 110 NanoDrop OneC with NanoDropQC Software User Guide Thermo Scientific This page is intentionally blank ...

Page 115: ...NanoDropQC Software User Guide 111 5 Maintenance Maintenance Schedule 112 Cleaning the Touchscreen 113 Maintaining the Pedestals 114 Decontaminating the Instrument 119 Maintaining the Cuvette Sampling System 121 Instrument Diagnostics 122 ...

Page 116: ...s with 0 5M HCl Recondition pedestals Every 6 Months Recondition pedestals Run Intensity Check Run Performance Verification Run Pedestal Image Check If you are experiencing an issue with your system refer to the troubleshooting information If the issue persists contact us If you are outside the U S A and Canada please contact your local distributor If your instrument requires maintenance or repair...

Page 117: ... do not clean the touchscreen with abrasive material such as paper towel apply excessive pressure spray liquid directly onto the touchscreen apply lubricant to the touchscreen slide mechanism To clean the touchscreen Gently wipe the touchscreen with a soft lint free cloth such as microfiber If necessary use a cleaner intended for glass LCD displays and follow the manufacturer s recommendations ...

Page 118: ...ydrofluoric acid HF on the pedestals Fluoride ions will permanently damage the quartz fiber optic cables To prevent damage from spills keep containers of liquids away from the instrument Do not use a squirt or spray bottle on or near the instrument as liquids will flow into the instrument and may cause permanent damage Do not attempt to remove the diaphragm around the lower pedestal as it is perma...

Page 119: ...edestal with a new laboratory wipe To clean the pedestals between users 1 Lift the arm and clean both pedestals with a new laboratory wipe 2 Pipette 3 5 µL DI H2O onto the lower pedestal 3 Lower the arm and wait 2 3 minutes 4 Lift the arm and clean both pedestals with a new wipe Tip When thorough cleaning is required for example to remove dried sample left on the pedestals substitute 0 5M HCl for ...

Page 120: ...lets on the lower pedestal to flatten out preventing proper formation of the liquid column when the arm is lowered The resulting spectrum may look rough or jagged If samples flatten out on the pedestal rather than beading up or forming a rounded droplet or the liquid column breaks during a measurement recondition the pedestals Supplies needed lint free laboratory wipes PR 1 pedestal reconditioning...

Page 121: ...he PR 1 compound to dry 3 Fold a clean laboratory wipe into quarters and use it to vigorously buff the surface of each pedestal Notice Support the instrument arm with one hand while you buff the upper pedestal to avoid damaging the arm Tip Black residue on the wipe is normal 4 Repeat step 3 with a new folded wipe until all residue is removed and the pedestals buff clean 5 Use canned air to remove ...

Page 122: ...Lower the arm and wait 2 3 minutes 3 Lift the arm and clean both pedestals with a new laboratory wipe 4 Pipette 3 µL DI H2O onto the lower pedestal 5 Lower the arm and wait 2 3 minutes 6 Lift the arm and clean both pedestals with a new wipe NOTICE Support the instrument arm with one hand while you buff the upper pedestal to avoid damaging the arm 7 Fold a clean laboratory wipe into quarters and us...

Page 123: ...uires maintenance or repair contact us or your local distributor NOTICE Do not use hydrofluoric acid HF on the pedestals Fluoride ions will permanently damage the quartz fiber optic cables To prevent damage from spills keep containers of liquids away from the instrument Do not use a squirt or spray bottle on or near the instrument as liquids will flow into the instrument and may cause permanent da...

Page 124: ... see Supplies needed onto the lower pedestal 3 Lower the arm and wait 2 3 minutes 4 Lift the arm and clean both pedestals with a new wipe 5 Pipette 3 5 µL DI H2O onto the lower pedestal 6 Lower the arm and wait 2 3 minutes 7 Lift the arm and clean both pedestals with a new wipe 1 Dampen a clean soft cloth or laboratory wipe with the diluted bleach solution see Supplies needed and use it to gently ...

Page 125: ...ach measurement Use cuvettes that are free of scratches and avoid fingerprints which may affect results NOTICE Do not use a squirt or spray bottle on or near the instrument as liquids will flow into the instrument and may cause permanent damage To maintain the cuvette sampling system Keep the instrument arm closed when the instrument is not in use Use canned air to remove any dust from the cuvette...

Page 126: ...rol options Intensity Check Run Intensity Check every 6 months to verify operation of the instrument s internal components The test measures the intensity of light from the xenon source through the instrument to verify that throughput wavelength accuracy and bias are within specifications The test is automatically performed using the pedestal and the cuvette optical paths Supplies needed lint free...

Page 127: ...Intensity Check If you are using the PC Control software from the Home screen select Intensity 5 On the instrument Tap Measure and wait for the measurements to complete Here is an example of a typical intensity check result screen If you are using the PC Control software select Run 6 To rerun the intensity check tap Measure 7 When finished tap End Experiment After the test is completed the results...

Page 128: ...ck If a yellow triangle appears next to the Bias indicator make sure the room is within the temperature specifications for the instrument If the Intensity Check fails again contact us Performance Verification Run Performance Verification every 6 months to confirm pathlength accuracy is within specifications Supplies needed lint free laboratory wipes deionized water DI H2O calibrated precision pipe...

Page 129: ...n performance verification 1 From the instrument home screen tap Diagnostics and then tap Performance Verification If you are using the PC Control software from the Home screen select Performance A message asks for target absorbance values Note The PV 1 solution comes in a single use ampoule Before you open the ampoule shake it vigorously and then allow the liquid to collect in the bottom portion ...

Page 130: ...lank 5 Lift the arm and clean both pedestals with a new wipe 6 Pipette 1 µL PV 1 solution onto the lower pedestal and start the sample measurement If Auto Measure is On lower arm If Auto Measure is off lower arm and tap Measure or from the PC Control software select Run After the measurement the software displays the results Here is an example of the performance verification result screen Note Vig...

Page 131: ...tion nine more times using a new 1 µL aliquot for each measurement and cleaning both pedestals after each measurement After each measurement a new sample result is added to the display Swipe the screen left to see a summary of the 10 sample results Swipe screen left to view detailed results Measured pathlengths Drag tab down up to see more less data ...

Page 132: ... details along with the overall test result After the tenth measurement a message indicates whether the instrument passed or failed performance verification 8 If the instrument failed immediately repeat step 6 using ten 2 µL aliquots of the PV 1 solution 9 When finished tap End Experiment and clean the pedestals with 3 5 µL DI H2O Performance test result ...

Page 133: ...9 After the test is completed the results are available from the Data Viewer see example below See Manage identifiers on the instrument for details To interpret performance verification results If your instrument failed performance verification and you repeated ten measurements using 2 uL aliquots contact us ...

Page 134: ...lower pedestal with a new laboratory wipe 2 Lower the arm 3 From the instrument home screen tap Diagnostics and then tap Pedestal Image Check If you are using the PC Control software from the Home screen select Pedestal Image 4 Tap Measure or select Run The instrument runs a series of tests to check pedestal position and image quality After the measurements are completed the results are displayed ...

Page 135: ...anoDrop OneC with NanoDropQC Software User Guide 131 6 Safety and Operating Precautions Contents Operating Precautions Safety Information NOTICE Be sure that all persons operating this system read the safety manual first ...

Page 136: ...her the plate or display become cracked or broken contact us for replacement Use solvents that are compatible with the instrument see Hazardous Materials Do not use hydrofluoric acid HF on the pedestals Fluoride ions will permanently damage the quartz fiber optic cables To prevent damage from spills keep containers of liquids away from the instrument Do not use a squirt or spray bottle on or near ...

Page 137: ... instrument itself The symbol indicates that there is additional safety information in the documentation and failure to heed the safety precautions could result in injury WARNING Indicates a hazardous situation which if not avoided could result in death or serious injury CAUTION Indicates a hazardous situation which if not avoided could result in minor or moderate injury NOTICE Follow instructions...

Page 138: ...cal shock in the vicinity Only qualified persons shall perform the related procedures Avoid fire hazard Do not test flammable or explosive samples Read and follow the associated instructions carefully Avoid eye injury If you see these symbols there is a risk of exposure to ultraviolet light which can harm your eyes if safety glasses are not worn Avoid Biohazard This icon informs of a biological ha...

Page 139: ...ocumentation the protection provided by the equipment may be impaired CAUTION Avoid personal injury Perform only those procedures described in the documentation If there are other problems contact us Any other service must be performed by trained personnel CAUTION Avoid shock hazard Do not remove the cover of the instrument All service to the instrument must be performed by trained personnel NOTIC...

Page 140: ...UTION Avoid shock hazard Only a qualified person using the appropriate measuring device shall check the line voltage current and frequency Only our trained and certified service representatives shall attempt to service a component that carries this symbol If a protective cover on a system component appears damaged turn off the system and secure it against any unintended operation Always examine th...

Page 141: ...hased locally Contact technical support for information about power conditioners and UPS Electrical Service Specifications The following table lists the specifications for electrical service Contact our service representative in your area if you have questions about the requirements Power Consumption Generally 50 more power should be available than the entire system including accessories typically...

Page 142: ...s state Volatile Solvents and Flammable Samples Compatible Solvents Most solvents typically used in life science laboratories are compatible with the fiber optic pedestals of all NanoDrop spectrophotometers However the high vapor pressure properties of some solvents may not be conducive to small volume measurements when using the pedestal for measurements on any of the NanoDrop instruments If you ...

Page 143: ...ease contact us Biohazard or Radioactive Materials and Infectious Agents Biological samples such as tissues body fluids infectious agents and blood of humans and other animals have the potential to transmit infectious diseases Wear appropriate protective equipment Individuals should be trained according to applicable regulatory and organization requirements before working with potentially infectio...

Page 144: ...ntaminated with biohazard or radioactive materials infectious agents or any other materials and or conditions that could constitute a health or injury hazard to employees Contact us if you have questions about decontamination requirements WARNING Reduce the risk associated with potentially infectious samples Do not spill samples on any of the instrument components If spill occurs disinfect the ext...

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