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Thermo Fisher Scientific

Thermo Scientific KingFisher

Flex User Manual

Chapter 10

References

bead-based microspheres

beads nucleic

acids

biopanning oligo-dT

cell enrichment

particles

cell purification

PCR product

cell separation

phage display

DNA

post PCR purification

DNA extraction

primer

DNA immobilization

processing

DNA isolation

processor

DNA purification

protein purification

DNA strand separation

proteomics

DNA template

purification

double-stranded DNA

RNA

genomics DNA

mRNA

genomics

recombinant protein purification

GST-tag proteins

RT-PCR

His-tag proteins

separation

immunomagnetic separation

silica-coated particles

immunoprecipitation streptavidin-coated

beads

KingFisher streptavidin-coated

particles

magnetic beads

superparamagnetic beads

magnetic capture

superparamagnetic microspheres

magnetic microspheres

Thermo Fisher Scientific

magnetic particles

Thermo Scientific

magnetic separation

Keywords for

web pages

Summary of Contents for KingFisher Flex

Page 1: ...KingFisher Flex ServiceManual Version1 1 ...

Page 2: ......

Page 3: ...view of content of KingFisher Flex service manual Section 2 Operating Instructions This is a copy of user manual Section 3 PCB Functional Description Section 4 Block Diagrams Section 5 Component Layouts Component PCB layout drawings Section 6 Schematic Diagrams Section 7 Software Describes KingFisher Flex software remote commands Section 8 Testing and Adjusting Explains how to adjust and test the ...

Page 4: ...1 2 Introduction Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 5: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Operating Instructions 2 1 Section 2 Operating Instructions This section is a copy of KingFisher Flex user manual ...

Page 6: ...2 2 Operating Instructions Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 7: ...Thermo Scientific KingFisher Flex User Manual DRAFT Rev 1 0 Cat no N07669 ...

Page 8: ...cientific Microplate Instrumentation Business products are fully guaranteed against defective parts and materials including defects caused by poor workmanship for a period of one year from the date of delivery Thermo Fisher Scientific will repair or replace defective parts or materials during the term of warranty at no extra charge for materials and labor provided that the products were used and m...

Page 9: ...Flex hardware and onboard software Refer to Chapter 7 Technical Specifications In Chapter 4 Routine Operation you find explanations of the processing principles and procedures and on how to use the KingFisher Flex internal software In Chapter 9 Troubleshooting Guide you find explanations of error and warning messages and a problem solving guide The user should be familiar with the contents of Chap...

Page 10: ...ning Hot surface risk of burns Warning Risk of body parts hair jewelry or clothing getting caught in a moving part Serial number Catalog number Date of manufacture Consult instructions for use WEEE symbol This product is required to comply with the European Union s Waste Electrical Electronic Equipment WEEE Directive 2002 96 EC A black label with the following text Figure 2 4 WARNING DISCONNECT SU...

Page 11: ...nual Warning Risk of electric shock Warning Biohazard risk Warning Risk of injury to the user s Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application Note Marks a hint important information that is useful in the optimum operation of the system or an item of interest Warning and other markings used in the documentation ...

Page 12: ...pecifically designed for the device Never open any other covers of the KingFisher Flex than the see through lid or the sliding door Figure 2 3 while the instrument is plugged into a power source Never force a microplate onto the instrument The KingFisher Flex is intended for laboratory research use only Observe proper laboratory safety precautions such as wearing protective clothing and following ...

Page 13: ...le processor 16 Principle of magnetic particle processing 17 Working with a magnetic rod 18 Collecting magnetic particles 18 Releasing magnetic particles 18 Washing magnetic particles 18 Incubation 18 Changing the volume during the magnetic particle processing 18 Installation 21 Delivery check 21 Unpacking 21 Checking delivery 22 Requirements 22 Precautions and limitations 23 Installation setups 2...

Page 14: ...me 48 DNA RNA Cell Protein Other 50 Instrument options 52 Device information 52 Computer interface 53 Language 53 Buzzer 54 Maintenance protocol 55 Screen saver 56 How to handle tip combs 57 How to start 59 Changing the heating block 64 Shutdown 66 Emergency situations 66 Maintenance 67 Regular and preventive maintenance 67 How to clean the turntable 67 How to clean the magnetic rods 69 How to cle...

Page 15: ...ges and warnings 83 Troubleshooting guide 84 Service request protocol 85 Certificate of Decontamination 86 Ordering Information 87 KingFisher Flex 87 List of accessories 87 List of spare parts 88 References 89 Keywords for web pages 89 Literature 90 Certificate of Decontamination 93 Thermo Scientific KingFisher Flex Feedback Form 95 Chapter 7 Chapter 8 Chapter 9 Chapter 10 Appendix A Appendix B ...

Page 16: ...ng the heating block into place 28 Figure 3 17 Shield plate prior to fitting 29 Figure 3 18 Inserting the shield plate 30 Figure 3 19 Fastening loosening the shield plate 30 Figure 3 20 Shield plate fitted 31 Figure 3 21 KingFisher Flex heads in their storage boxes 32 Figure 3 22 Inserting the KingFisher Flex head 33 Figure 3 23 KingFisher Flex head for KingFisher 24 Deep Well plate fitted34 Figur...

Page 17: ...tes loaded 62 Figure 4 42 Tip comb in the tip comb holder 62 Figure 4 43 Shield plate in function 63 Figure 4 44 KingFisher Flex plates 63 Figure 4 45 Inserting the heating block 64 Figure 4 46 Pressing the heating block into place 65 Figure 4 47 Four different heating blocks available 65 Figure 5 48 Unfastening the two finger screws 68 Figure 5 49 Removing the turntable 68 Figure 5 50 Black spill...

Page 18: ...w 43 Table 4 4 Processing volumes vs plate types and magnetic heads 64 Table 4 5 Heating blocks vs plate types 65 Table 6 6 General specifications 75 Table 6 7 Performance specifications 75 Table 8 8 Error messages reported 83 Table 8 9 Actions taken against error messages and warnings 84 Table 9 10 Instrument catalog number 87 Table 9 11 Codes for accessories 87 Table 9 12 Codes for spare parts 8...

Page 19: ... particle processor Figure 1 1 is designed for automated transfer and processing of magnetic particles in microplate format The patented technology of the KingFisher Flex system is based on the use of magnetic rods covered with a disposable specially designed tip comb and plates The instrument functions without any dispensing or aspiration parts or devices Samples and reagents including magnetic p...

Page 20: ...essing and purification Binding and elution are enhanced due to the heating option Enables concentration of the sample during processing Facilitates a good collection of bead bound sample due to the efficiency of the magnet Enables a quicker reaction and a more efficient wash due to the technology of moving magnetic particles instead of liquids No carryover No cross contamination Facilitates the w...

Page 21: ...nt views of the KingFisher Flex instrument are shown in Figure 2 2 and Figure 2 3 Figure 2 2 KingFisher Flex front view without see through lid Figure 2 3 KingFisher Flex front view with see through lid and plates Instrument layout Front view Heating block Display Keypad Plate station 1 8 Shield plate Loading station Turntable Interchangeable KingFisher Flex head See through lid Sliding door open ...

Page 22: ...etic rods 24 or 96 pieces and the other one for the plastic tip comb Up to eight plates can be simultaneously on the turntable However during one sample processing the protocol enables the use of more than eight plates in total One tip comb contains 24 or 96 tips used for processing 24 or 96 samples at a time Before starting the magnetic particle processing via the keypad and display the samples a...

Page 23: ...on The operating principle employed is MPP inverse magnetic particle processing technology Figure 2 7 Rather than moving the liquids the magnetic particles are moved from plate to plate containing specific reagents in contrast to the external magnet method Magnetic particles are transferred with the aid of magnetic rods covered with a disposable specially designed plastic tip comb Figure 2 6 KingF...

Page 24: ...ed off and the tip comb is lowered into the next plate containing a reagent Magnetic particles are released by moving the tip comb up and down several times at considerably high speed until all the particles have been mixed with the substance in the next reaction Washing the magnetic particles is a frequent and an important processing phase Washing is a combination of the release and collection pr...

Page 25: ...Functional Description Working with a magnetic rod Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 19 Figure 2 8 A concentration step during magnetic particle processing ...

Page 26: ...Functional Description Working with a magnetic rod 20 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific ...

Page 27: ...s are sent with the instrument and are immediately available when you open the package Unpacking instructions KingFisher Flex Feedback Form Warranty Certificate card Packing instructions Packing list KingFisher Flex User Manual and Quick Reference Guide Transportation discrepancy report BindIt Software package Caution Do not touch or loosen any screws or parts other than those specifically designa...

Page 28: ...lp obtain restitution from the carrier Upon receipt of the carrier s inspection report arrangements will be made for repair or replacement If any parts are damaged contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy When you set up your KingFisher Flex avoid sites of operation with excess dust vibrations strong magnetic fields direct sunlight or UV light draft...

Page 29: ...Fisher Flex should not be kept in close proximity to magnetic tapes computer discs or other magnetic storage systems such as credit cards as these can be damaged by the strong magnetic field of the KingFisher Flex heads Do not hold the KingFisher Flex heads close to a PC display since this may cause damage to the display Do not use metal tools when handling KingFisher Flex heads Warning This produ...

Page 30: ...ly hold of the lock and press tightly on both sides of the lock according to Figure 3 10 so that the lock ends are released Keep hold of the tip comb holder and lock simultaneously and start gradually lifting the holder Figure 3 10 Removing the transport lock of the tip comb holder 2 Lift the tip comb holder higher and continue pressing the transport lock of the tip comb holder Remove the transpor...

Page 31: ...nt refer to How to refit the transport lock of the tip comb holder on page 36 Figure 3 11 Tip comb holder transport lock removed 3 The transport lock of the heating block is removed according to Figure 3 12 Unscrew the transport lock screw counterclockwise Figure 3 12 Lift the screw with the label attached Figure 3 13 Figure 3 12 Removing the transport lock of the heating block A Two eccentric pin...

Page 32: ...ortation of the instrument Both the transport locks have been successfully removed When relocating the instrument refer to How to refit the transport lock of the heating block on page 35 and How to refit the transport lock of the tip comb holder on page 36 1 Connect the mains supply cable to the mains power connector Figure 3 14 at the bottom left of the back side panel If you need to use any othe...

Page 33: ...ply cable and serial cable connected This section describes the installation setups of the heating block shield plate interchangeable KingFisher Flex heads and the see through lid Fastening changing the heating block is controlled by the software To fit the heating block choose the change_heatblock protocol under the Maintenance menu by using the up and down cursor keys Then press START The heatin...

Page 34: ...inds of interchangeable heating blocks available for KingFisher 24 Deep Well plates Microtiter 96 Deep Well plates KingFisher 96 plates and PCR plates Table 3 1 Table 3 1 Plates vs heating adapter s Plate Description Heating adapter s Description KingFisher 24 Deep Well plate 200 µl 5 mL Heating block for KingFisher 24 Deep Well plate Microtiter 96 Deep Well plate 50 1000 µl Heating block for Micr...

Page 35: ...late 200 µl 5 mL Microtiter 96 Deep Well plate 50 1000 µl KingFisher 96 plate 20 200 µl PCR plate 20 100 µl recommended filling volume To install the shield plate follow these steps 1 Ensure that the power is switched OFF 2 The shield plate has to be installed prior to use of the instrument Use a hexagonal screwdriver Allen key to fasten the two screws Figure 3 17 Figure 3 17 Shield plate prior to...

Page 36: ... Manual Thermo Fisher Scientific Figure 3 18 Inserting the shield plate 4 When fastening the two plate retaining screws Figure 3 19 be careful not to drop them inside the instrument Figure 3 20 shows the shield plate installed Figure 3 19 Fastening loosening the shield plate ...

Page 37: ...ll have corresponding disposable plastic tip combs Table 3 2 Note that KingFisher Flex heads do not fit into the KingFisher 96 and vice versa Caution Do not place the KingFisher Flex heads on top of the instrument or any metal surfaces Keep the KingFisher Flex heads always in their respective plastic boxes when not in use It is very important to keep the KingFisher Flex heads away from each other ...

Page 38: ...or KingFisher 96 plate KingFisher Flex head for PCR plate Tip comb for PCR plate When not in use keep the KingFisher Flex heads always in their respective plastic storage boxes Figure 3 21 Figure 3 21 KingFisher Flex heads in their storage boxes Caution The KingFisher Flex should not be kept in close proximity to magnetic tapes computer discs or other magnetic storage systems such as credit cards ...

Page 39: ...aged if it comes in close contact with a strong magnetic field To insert KingFisher Flex heads choose the change_magnet protocol under the Maintenance menu by using the up and down cursor keys Then press START Insert the KingFisher Flex head so that the three pins shown with black arrows slot into place Figure 3 22 and Figure 3 23 When you remove the KingFisher Flex head be careful not to damage t...

Page 40: ... see through lid can be absent Figure 3 24 The closed door protects the processing against environmental contamination Figure 3 24 See through lid fitted First switch the instrument ON The instrument performs initialization tests and adjustments The display quickly shows the internal software version This happens when the initialization tests and adjustments have been completed See through lid Ope...

Page 41: ...sport locks the transport lock of the heating block and that of the tip comb holder To refit the transport lock of the heating block follow these steps 1 Remove the heating block in the following way First choose the change_heatblock protocol Then press START Remove the heating block by pulling it out Press the START key when the task has been completed 2 Switch off the power 3 Take the transport ...

Page 42: ... transport locks refitted Figure 3 26 Refitting the transport lock of the heating block B To refit the transport lock of the tip comb holder follow these steps 1 The transport lock of the tip comb holder is refitted according to Figure 3 27 through Figure 3 28 Press the transport lock from both sides and insert the two eccentric pins back into place into their respective holes Figure 3 27 Caution ...

Page 43: ...igure 3 27 Fitting the transport lock of the tip comb holder A 2 Press the tip comb holder transport lock ends together with both hands and insert all the way down to lock position Figure 3 28 Note that the turntable can move slightly although the transport lock is fitted Figure 3 28 Fitting the transport lock of the tip comb holder B ...

Page 44: ...ument for transportation 38 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Figure 3 29 Both transport locks refitted Figure 3 29 shows both the transport lock of the heating block and the tip comb holder refitted ...

Page 45: ...tion before you do anything else Always use correct volumes on the plates because the movements of the tip comb are conducted according to the volumes Use for self testing is excluded Do not touch or loosen any screws or parts other than those specifically designated in the instructions Doing so might cause misalignment and will invalidate the instrument warranty Never open any other cover of the ...

Page 46: ...shown in Figure 4 30 Figure 4 30 Keyboard of the KingFisher Flex The relevant keys and control buttons are described in detail below The arrow keys are used to select the next protocol and to navigate in the display To accept the selection To initiate the run To confirm a performed step in the protocol for example plate loading or removal To pause terminate the processing step In short STOP paused...

Page 47: ...ls and Maintenance In routine use you mainly navigate in the Factory User protocols menu In advanced level options there is one extra menu Maintenance You can navigate between these three menus using the Left and Right arrow keys The main view row is either colored active or uncolored different colored inactive All the descriptive icons used in the main view are shown in Table 4 3 below The info t...

Page 48: ...ms for example the icon and main view row in the main view changes when they are selected active inactive To move from one menu to another make sure you are in the main view of one of the menus and use the Left and Right arrow keys The main views of each menu tab are shown below Active item Available buttons and their function ...

Page 49: ...view Menu Icon Function Favorite factory protocol name on page 45 DNA RNA Cell Protein Other on page 47 Cell Protein Other Favorite user protocol name on page 48 DNA RNA Cell Protein Other on page 50 Cell Protein Other Alternatively Human Animal Plant Bacteria Virus Device information on page 52 Computer interface on page 53 Continued ...

Page 50: ...Routine Operation Control panel 44 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Menu Icon Function Language on page 53 Buzzer on page 54 Maintenance protocol on page 55 Cont ...

Page 51: ...her For more information refer to the BindIt Software User Manual Cat no N07974 This section describes procedures related to the KingFisher Flex internal software This section describes the factory set protocols They are found in the Factory protocols menu Go to the Factory protocols menu You can set your favorite protocol If you do not the last used protocol will be shown Use the Up and Down arro...

Page 52: ...lex User Manual Thermo Fisher Scientific Use the Up and Down arrow keys to select more Press START to run the protocol Press OK to view the details of the protocol Use the Up and Down arrow keys to select more Press STOP to go back Press OK to accept the default protocol ...

Page 53: ... Scientific KingFisher Flex User Manual 47 Go to the Factory protocols menu Select for example the DNA RNA row Press OK Use the Up and Down arrow keys to select the protocol Press START to run the protocol Press OK for details of the protocol DNA RNA Cell Protein Other ...

Page 54: ...Favorite factory protocol name on page 45 This section describes the user s own protocols and is in many ways similar to the section on factory protocols The user protocols are described in the User protocols menu Go to the User protocols menu You can set your favorite protocol If you do not the last used protocol will be shown Use the Up and Down arrow keys to select the main item row Press START...

Page 55: ... Flex User Manual 49 Press OK for details of the protocol Use the Up and Down arrow keys to select more Press START to run the protocol Press OK to view the details of the protocol Use the Up and Down arrow keys to select more Press STOP to go back Press OK to set the protocol as default ...

Page 56: ... Flex User Manual Thermo Fisher Scientific Use the Up and Down arrow keys to select more Press START to run the protocol Press OK to view the details of the protocol Go to the User protocols menu Select for example the DNA RNA row Press OK DNA RNA Cell Protein Other ...

Page 57: ...ific Thermo Scientific KingFisher Flex User Manual 51 Use the Up and Down arrow keys to select the protocol Press START to run the protocol Press OK for details of the protocol Use the Up and Down arrow keys to select more Press START to run the protocol ...

Page 58: ...ion describes the instrument parameters All these parameters are set in the Maintenance menu The values shown in the Maintenance menu remain in the instrument memory and are instrument specific not protocol specific The device information shows the name of the instrument the internal software version and the serial number of the instrument unit Go to the Maintenance menu Press OK Press OK to exit ...

Page 59: ... row Press OK Use the Up and Down arrow keys to select the computer interface in use The default is Not active Press OK You can set the language of the internal software Go to the Maintenance menu Select the Language selection row Computer interface Language This selection also has to be done when you use the instrument through BindIt Software Refer to Chapter 2 Installing the Software in the Bind...

Page 60: ... keys to select the language in use The available languages are English Deutsch Français Español and Portuguese The default language is English Press OK You can choose whether or not the instrument produces a sound for different functions Note that all functions make a certain sound Go to the Maintenance menu Select the Buzzer on off row Buzzer ...

Page 61: ... arrow keys to select whether the buzzer audible sound is On or Off Press OK You can set which protocol is automatically selected as the maintenance protocol when the KingFisher Flex is powered on Go to the Maintenance menu Select the Maintenance protocol row Press OK All available maintenance protocols will be displayed Maintenance protocol ...

Page 62: ...nd Down arrow keys to select the maintenance protocol Press START to run the desired protocol Press OK to go back The KingFisher Flex screen saver shown below appears when the instrument has not been used for 20 minutes You can close the screen saver and return to the previous display by pressing any key Screen saver ...

Page 63: ...bs Figure 4 32 Figure 4 32 Tip comb package with two tip combs 2 Unpack the package by separating the two tip combs Figure 4 33 and placing one of the tip combs onto the KingFisher 96 plate tip plate Figure 4 37 and Figure 4 38 Figure 4 33 Separating the two tip combs 3 Place the other unused tip comb onto another plate for storage to avoid bending of the tip combs Figure 4 34 to ensure proper ins...

Page 64: ...tip comb package where the unattached tip comb is packed onto a KingFisher 24 Deep Well plate Figure 4 35 Figure 4 35 24 deep well tip comb package 2 Unpack the package 3 Before you take the tip comb into use press the tip comb evenly against the enclosed 24 deep well plate with the aid of a magnetic head or another object To handle PCR tip combs correctly follow these steps 1 Take a PCR tip comb ...

Page 65: ...e 2 3 3 Load the plates in the order that the protocol requests Figure 4 39 through Figure 4 41 Place the A1 well of the plate so that it is in the upper right corner The first A1 row is consequently always in the inner circle Once you have loaded the requested plates into the plate stations press START The tip comb always has to be placed manually onto a KingFisher plate Figure 4 37 through Figur...

Page 66: ... The loading position that is plate station 4 is labeled The eight plate stations and the A1 positions of the eight plate stations are clearly marked on the turntable When the instrument is in its basic position plate station 1 is under the KingFisher Flex head After the protocol has been run note that the turntable may stop in a different position than the basic position Loading postion plate sta...

Page 67: ...Routine Operation How to start Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 61 Figure 4 39 Loading the tip plate Figure 4 40 Loading the plates A1 corner Tip plate ...

Page 68: ...ded 4 The tip comb is automatically locked onto the tip comb holder from the tip plate Figure 4 42 Figure 4 42 Tip comb in the tip comb holder 5 When the turntable moves the shield plate moves over the plate underneath Figure 4 43 forming a protective cover Tip comb holder Magnetic rods Tip plate Tip comb in the tip comb holder ...

Page 69: ...ccording to the protocol request Confirm each plate removal by pressing the START key Note that the plate containing your samples is removed first 8 Press the STOP key after completing the run For startup refer to Switching on on page 40 Figure 4 44 KingFisher Flex plates The KingFisher Flex is compatible with KingFisher 24 Deep Well plates Microtiter 96 Deep Well plates KingFisher 96 plates and P...

Page 70: ... Flex head for PCR plate 20 200 µl 20 100 µl Changing the heating block is controlled by the software To change the heating block choose the change_heatblock protocol by using the up and down cursor keys Then press START The protocol requests you to remove the magnets and in the end to insert them The heating block will thus rise Remove the heating block and then replace it Figure 4 45 and Figure ...

Page 71: ...our different kinds of interchangeable heating blocks available for KingFisher 24 Deep Well plates Microtiter 96 Deep Well plates KingFisher 96 plates and PCR plates Figure 4 47 and Table 4 5 Figure 4 47 Four different heating blocks available Table 4 5 Heating blocks vs plate types Heating block Plate KingFisher 24 Deep Well plate Microtiter 96 Deep Well plate KingFisher 96 plate PCR plate Heatin...

Page 72: ...urface and the adjacent instrument surface with a soft cloth or tissue paper moistened with distilled water a mild detergent SDS sodium dodecyl sulfate or soap solution 3 If you have spilt infectious agents on the turntable disinfect with 70 ethanol or some other disinfectant see Decontamination procedure on page 70 In case there is any abnormal situation during operation such as fluids spilling i...

Page 73: ...line solutions solvents acids or alkaline solutions from outer surfaces to prevent damage Caution Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer Do not expose the surfaces to concentrated acids or concentrated alcohols for prolonged periods of time as damage may occur Clean the display areas with a mild laboratory deterg...

Page 74: ...n SDS soap solution or alcohol The turntable can be detached for cleaning purposes Figure 5 48 through Figure 5 50 Unscrew the two finger screws Figure 5 48 Note that the screws still remain attached to the turntable when the screws are unfastened Figure 5 48 Unfastening the two finger screws Lift the turntable off Figure 5 49 Figure 5 49 Removing the turntable Alignment stud ...

Page 75: ...d wipe the magnetic rods using a soft cloth or tissue paper soaked in a mild detergent solution SDS soap solution or alcohol Caution The KingFisher Flex should not be kept in close proximity to magnetic tapes computer discs or other magnetic storage systems such as credit cards as these can be damaged by the strong magnetic field of the KingFisher Flex heads Do not hold the KingFisher Flex heads c...

Page 76: ...ation procedure is only recommendable when infectious substances have been in direct contact with any part s of the instrument If there is a risk of contamination with biohazardous material the procedure recommended below or some other corresponding decontamination procedure must be performed It is strongly recommended to perform the complete decontamination procedure before relocating the instrum...

Page 77: ...n the instrument using a mild detergent 10 Remove any stains using 70 ethanol 11 After performing this decontamination procedure enclose a signed and dated Certificate of Decontamination both inside the transport package and attached to the outside of the package see Appendix A Certificate of Decontamination To pack for service follow the guidelines presented below Caution It is important that the...

Page 78: ...peratures It is recommended to maintain and service the instrument regularly every 12 months on a contract basis by the manufacturer s trained service engineers This ensures that the product is properly maintained and gives trouble free service Contact the Thermo Fisher Scientific technical service department for more details If the KingFisher Flex has to be disposed of follow the guidelines below...

Page 79: ...posed of or recycled through them Further information on Thermo Fisher Scientific s compliance with these Directives the recyclers in your country and information on Thermo Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www thermo com WEEERoHS Regarding the original packaging and packing materials use the recycling operators known to...

Page 80: ...Maintenance Disposal of the instrument 74 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific ...

Page 81: ...ures up to 31 C decreasing linearly to 50 relative humidity at 40 C Indoor use only Transportation conditions 40 C to 70 C packed in transport packaging Storage conditions 25 C to 50 C packed in transport packaging Mains power supply 100 240 Vac 50 60 Hz nominal Automatic voltage detection Power consumption 150 VA max 55 VA standby Heat dissipation 341 BTU max Computer interface USB or serial RS 2...

Page 82: ...for KingFisher 24 Deep Well plate for Microtiter 96 Deep Well plate for KingFisher 96 plate for PCR plate Heating block temperature From 4 C above ambient temperature to 115 C Heating accuracy 6 C up to 80 C with KingFisher 96 plate up to 96 C with PCR plate instrument in RT Keyboard Display START PAUSE STOP OK TURNTABLE ROTATING CLOCKWISE TURNTABLE ROTATING COUNTERCLOCKWISE four cursor keys LCD T...

Page 83: ...ironments EN 61000 6 1 2007 Generic standards Immunity standard for residential commercial and light industrial environments EN 61326 1 2006 Product family standard Electrical equipment for measurement control and laboratory use EN 61326 2 6 2006 Product family standard Electrical equipment for measurement control and laboratory use In vitro diagnostic IVD medical equipment Test standards Performa...

Page 84: ...Technical Specifications In conformity with the requirements 78 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific ...

Page 85: ...all three kinds of magnets for example KingFisher 96 plates can be used together in a protocol with either Microtiter 96 Deep Well plates or PCR plates Q3 Will the magnet get weaker If so how long can they be used A3 The KingFisher Flex magnets are made of material that is very stable The magnetic field will not get weaker However extreme mechanical force or heating can cause damage to the magnets...

Page 86: ...eating block work A7 The heating block is located inside the instrument and can be used automatically during the protocol All KingFisher Flex plates can be heated using specially designed interchangeable heating blocks Any number of heating steps can be added to the protocol During the protocol when the protocol enters the heating step the plate is automatically moved to the dedicated heating posi...

Page 87: ... to sediment the particles to the bottom of the plate Q12 What if the magnetic particles are attached to the tip combs after the run A12 This happens sometimes but it will not affect the yield because the sample has been released from the particles Q13 Are the volumes of reagents in each well critical A13 It is strongly recommended that you keep the specified volumes within the defined limits to a...

Page 88: ...Frequently Asked Questions Q As 82 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific ...

Page 89: ... 3 The turntable rotating mechanism is out of position The turntable position is in error 4 The magnetic head holder lifting mechanism is out of position The magnetic tips lift position is in error 5 The heating block lifting mechanism is out of position The heater lift position is in error 6 The shield plate turning mechanism is out of position The shield plate position is in error 7 Serial numbe...

Page 90: ... taken against error messages and warnings Code Error message Action 0 No error 1 The command was not recognized as a valid command Contact authorized technical service 2 The tip comb holder lifting mechanism is out of position Switch the instrument OFF and ON and try again If the error appears during initialization or is otherwise repeated contact service 3 The turntable rotating mechanism is out...

Page 91: ...out any prior contact It is imperative to indicate the fault and nature of the required service This will ensure a faster return of the instrument to the customer Your local Thermo Fisher Scientific representative or distributor will take care of sending a complaint form that is the Warranty Claim Technical Sheet to the Thermo Fisher Scientific technical service department The Warranty Claim Techn...

Page 92: ...y a dated and signed Certificate of Decontamination which must be both enclosed and attached to the outside of the package containing the instrument Refer to Appendix A Certificate of Decontamination and Decontamination procedure on page 70 Failure to confirm decontamination will incur additional labor charges or at worst the items will be returned for proper cleaning Before returning any instrume...

Page 93: ...tem Quantity N07669 KingFisher Flex User Manual 1 N07974 BindIt Software User Manual 1 5189010 BindIt Software for KingFisher Instruments 1 5189009 BindIt Software for KingFisher Instruments CD 1 24074410 KingFisher Flex 96 PCR head 1 24074420 KingFisher Flex 96 KF head 1 24074430 KingFisher Flex 96 DW head 1 24074440 KingFisher Flex 24 DW head 1 24075410 KingFisher Flex 96 PCR heating block 1 240...

Page 94: ...cientific KingFisher Flex User Manual Thermo Fisher Scientific Table 9 12 Codes for spare parts Code Item Quantity 2305290 Serial cable F9 F25 for RS 232C port 1 N04001 USB A B device cable 1 Longer USB cables available from PC stores List of spare parts ...

Page 95: ...protein purification DNA strand separation proteomics DNA template purification double stranded DNA RNA genomics DNA mRNA genomics recombinant protein purification GST tag proteins RT PCR His tag proteins separation immunomagnetic separation silica coated particles immunoprecipitation streptavidin coated beads KingFisher streptavidin coated particles magnetic beads superparamagnetic beads magnetic...

Page 96: ...le based techniques Overview In Eds Robinson R K Batt C A and Patel P D Encyclopaedia of Food Microbiology Academic Press London UK Pp 1088 1094 Ginsberg S D 2002 High quality mRNA extraction with KingFisher for molecular fingerprinting Focus 1 5 Ginsberg S D and Che S 2002 RNA Amplification in Brain Tissues Neurochem Res 27 10 981 992 Hawkins D J O Connor Morin T Roy A and Santillan C 1994 DNA pu...

Page 97: ...n J Marttila H and Viljanen M K 2001 Automated purification of Borrelia burgdorferi s 1 PCR products with KingFisher magnetic particle processor prior to genome sequencing Journal of Magnetism and Magnetic Materials 225 134 137 Portelius E Tran A J Andreasson U Persson R Brinkmalm G Zetterberg H Blennow K and Westman Brinkmalm A 2007 Characterization of amyloid β peptides in cerebrospinal fluid by...

Page 98: ...G 2005 The survival of motor neurons protein determines the capacity for snRNP assembly Biochemical deficiency in spinal muscular atrophy Molecular and Cellular Biology 25 5543 5551 Ward L I Fenn M G E and Henry C M 2004 A rapid method for direct detection of Polymyxa DNA in soil Plant Pathology 53 485 490 Ye J D Tereshko V Frederiksen J K Koide A Fellouse F A Sidhu S S Koide S Kossiakoff A A and ...

Page 99: ...contaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological Radioactive Specific information about contaminants Decontamination procedure1 Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the u...

Page 100: ...Certificate of Decontamination 94 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific ...

Page 101: ...home page Date of purchase Your application area Dr Mr Mrs Ms Job title Position Surname block capitals First name block capitals Internet e mail address Excellent Above expectations As expected Below expectations Comments Instrument installation Ease of use Flexibility User manual Software Customer support Overall Additional instrument and or software features desired Did you encounter any proble...

Page 102: ...Thermo Scientific KingFisher Flex Feedback Form 96 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific ...

Page 103: ...ct magnetic particles Figure 4 42 The rods do not collect the particles on their own the magnetic rods must always be protected by a tip comb plate The disposable plates 1 8 where all the reagents and samples are located and where the processing takes place Four different types of 24 and 96 well plates Figure 4 44 that can be used are as follows KingFisher 24 Deep Well plate 200 µl 5 mL Microtiter...

Page 104: ...Glossary 98 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific ...

Page 105: ...l 71 83 cleaning the reagent reservoir 71 79 81 82 cleaning the tubings 71 73 79 column selection 41 44 column row selection column selection 41 full plate 40 row selection 35 40 43 selected wells 44 computer interface 35 60 94 consumables 2 control panel 32 91 100 current protocol 109 D dead volume 91 decontamination 69 72 83 84 85 86 87 88 101 105 109 default 38 39 40 45 46 47 48 49 50 51 58 64 ...

Page 106: ...uer connector 23 M maintenance 3 6 31 33 69 71 72 73 76 77 88 92 101 manifold 24 29 72 73 77 81 82 87 95 103 109 transport plug assembly 24 87 markings 109 N naming a protocol 35 45 54 nanodispensing 31 navigating 32 34 O opening an existing protocol 57 operation principle 14 operational check 25 ordering information 3 103 P packing instructions 19 88 packing list 19 plate 13 14 91 104 carrier 22 ...

Page 107: ...rial number 59 88 96 service 69 71 72 84 87 88 97 100 101 103 contracts 72 88 setting the column volume 41 setting the row volume 43 shaking 35 47 shutdown 68 72 specifications 3 88 89 91 92 93 speed 15 30 39 46 47 48 63 65 68 95 109 startup 33 35 36 53 58 61 96 protocol 35 58 61 stopping the protocol 32 symbols and markings 4 5 6 T technical specifications 3 91 tip 33 75 77 91 109 tip washing 33 ...

Page 108: ...102 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific ...

Page 109: ...Thermo Fisher Scientific Thermo Scientific KingFisher Flex User Manual 103 Notes ...

Page 110: ...104 Thermo Scientific KingFisher Flex User Manual Thermo Fisher Scientific Notes ...

Page 111: ...CB assembly MAIN 02 N07539 Refer to Section 3 1 on page 3 3 PCB assembly RT 01 2006180 Refer to Section 3 2 on page 3 4 PCB assembly MM 01 2006190 Refer to Section 3 3 on page 3 4 PCB assembly HEAT 01 2006200 Refer to Section 3 4 on page 3 5 PCB assembly MDCUI 02 N06407 Refer to Section 3 5 on page 3 5 PCB assembly HM 01 2006320 Refer to Section 3 6 on page 3 5 ...

Page 112: ...3 2 PCB Functional Description Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 113: ...h folio cable from connector X31 to X2 Shield PCB assembly MAIN 02 is connected to heating block shield motor from connector X33 Stepper driver N20 is for shield 3 1 2 Temperature Measurement and Control PCB assembly MAIN 02 is connected to PCB assembly HEAT 01 with folio flat cable from connector X13 to X3 PCB assembly MAIN 02 is connected to PCB assembly HM 01 with folio cable from connector X35...

Page 114: ...tor X34 from which fan assembly 2103900 is connected The fan assembly is installed to heater blower plate 3 2 PCB Assembly RT 01 PCB assembly RT 01 receives information from position sensors of turntable PCB assembly MAIN 02 controls turntable motor based on information received from RT 01 PCB assembly RT 01 has four connectors X1 Flat cable interface to PCB assembly MAIN 02 X5 Motor cable interfa...

Page 115: ...one connector X3 Folio cable interface to PCB assembly MAIN 02 X13 Connections to three resistors 3 5 PCB Assembly MDCUI 02 PCB assembly MDCUI 02 is a board for LCD and keyboard It is also connected to PCB assembly MAIN 02 X2 to PCB assembly MAIN 02 X17 X1 to LCD X3 to keyboard 3 6 PCB Assembly HM 01 PCB assembly HM 01 has two connectors X9 Folio cable interface to PCB assembly MAIN 02 X35 X12 Mot...

Page 116: ...3 6 PCB Functional Description Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 117: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Block Diagrams 4 1 Section 4 Block Diagrams Subject Document no Page PCB assembly MAIN 02 block diagram See Figure 4 1 on page 4 3 ...

Page 118: ...4 2 Block Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 119: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Block Diagrams 4 3 Figure 4 1 PCB assembly MAIN 02 block diagram ...

Page 120: ...4 4 Block Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 121: ...assembly MAIN 02 N07586 03X top See Figure 5 1 on page 5 3 PCB assembly RT 01 710 02X 02 See Figure 5 2 on page 5 5 PCB assembly MM 01 710 03X 01 See Figure 5 3 on page 5 6 PCB assembly HEAT 01 710 04X 02 See Figure 5 4 on page 5 7 PCB assembly MDCUI 02 D05741 04X See Figure 5 5 on page 5 8 PCB assembly HM 01 710 06X 02 See Figure 5 6 on page 5 9 ...

Page 122: ...5 2 Component Layouts Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 123: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Component Layouts 5 3 Figure 5 1 PCB assembly MAIN 02 top view N07586 03X ...

Page 124: ...motor driver X12 2305950 Power CHAMP8 cable assembly to power supply X13 1295300 HEAT folio cable to PCB assembly HEAT 01 X14 2006180 PCB assembly RT 01 Flat cable to PCB assembly RT 01 X15 N02023 Head motor assembly to head lift X16 2305960 Power 24V cable assembly to power supply incubation X17 N06407 PCB assembly MDCUI 02 Flat cable to LCD X20 2305190 RS cable assembly to RS 232 port X31 129528...

Page 125: ...2 PCB assembly RT 01 2006180 PCB assembly RT 01 Pos Part Description Remarks X1 Flat cable to PCB assembly MAIN 02 X14 on PCB assembly MAIN 02 X5 N07952 Turntable motor assembly X6 2305200 Track sensor assembly Turntable pos sensor 1 X7 2305200 Track sensor assembly Turntable pos sensor 2 710 02X 02 ...

Page 126: ...embly MM 01 Pos Part Description Remarks V1 Read Head GEH21A1 Magnet lift position sensor X2 1295280 Magnet lift cable assembly to PCB assembly MAIN 02 X31 X8 2306020 Magnet motor assembly X10 2306000 Switch cable to magnet plate head pos sensor micro switch X11 2306010 Switch cable 2 to tip comb pos sensor 2 micro switches 710 03X 01 ...

Page 127: ... Fisher Scientific Service Manual Version 1 1 Component Layouts 5 7 Figure 5 4 PCB assembly HEAT 01 2006200 PCB assembly HEAT 01 Pos Part Description Remarks X3 1295300 HEAT folio cable to PCB assembly MAIN 02 X13 710 04X 02 ...

Page 128: ...n 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex Figure 5 5 PCB assembly MDCUI 02 N06407 PCB assembly MDCUI 02 Pos Part Description Remarks X2 Flat cable to PCB assembly MAIN 02 X17 on PCB assembly MAIN 02 D05741 04X ...

Page 129: ...fic Service Manual Version 1 1 Component Layouts 5 9 Figure 5 6 PCB assembly HM 01 2006320 PCB assembly HM 01 Pos Part Description Remarks X9 1295290 HM folio cable to PCB assembly MAIN 02 X35 X12 21040990 Stepper motor assembly 710 06X 02 ...

Page 130: ...5 10 Component Layouts Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 131: ...rature sensor See Figure 6 8 on page 6 10 Display interface See Figure 6 9 on page 6 11 Head lift See Figure 6 10 on page 6 12 Turntable See Figure 6 11 on page 6 13 Shield See Figure 6 12 on page 6 14 Heating block See Figure 6 13 on page 6 15 Magnet lift See Figure 6 14 on page 6 16 PCB assembly RT 01 710 02S 02 See Figure 6 15 on page 6 17 PCB assembly MM 01 710 03S 01 See Figure 6 16 on page 6...

Page 132: ...6 2 Schematic Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 133: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Schematic Diagrams 6 3 Figure 6 1 PCB assembly MAIN 02 D06082 02 1 14 ...

Page 134: ...6 4 Schematic Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex Figure 6 2 Controller D06082 02 2 14 ...

Page 135: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Schematic Diagrams 6 5 Figure 6 3 Programmable logic D06082 02 3 14 ...

Page 136: ...6 6 Schematic Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex Figure 6 4 USB D06082 02 4 14 ...

Page 137: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Schematic Diagrams 6 7 Figure 6 5 Power D06082 02 5 14 ...

Page 138: ...6 8 Schematic Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex Figure 6 6 Position sensors D06082 02 6 14 ...

Page 139: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Schematic Diagrams 6 9 Figure 6 7 Incubation D06082 02 7 14 ...

Page 140: ...6 10 Schematic Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex Figure 6 8 Temperature sensor D06082 02 8 14 ...

Page 141: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Schematic Diagrams 6 11 Figure 6 9 Display interface D06082 02 9 14 ...

Page 142: ...6 12 Schematic Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex Figure 6 10 Head lift D06082 02 10 14 ...

Page 143: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Schematic Diagrams 6 13 Figure 6 11 Turntable D06082 02 11 14 ...

Page 144: ...6 14 Schematic Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex Figure 6 12 Shield D06082 02 12 14 ...

Page 145: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Schematic Diagrams 6 15 Figure 6 13 Heating block D06082 02 13 14 ...

Page 146: ...6 16 Schematic Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex Figure 6 14 Magnet lift D06082 02 14 14 ...

Page 147: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Schematic Diagrams 6 17 Figure 6 15 PCB assembly RT 01 710 02S 02 ...

Page 148: ...6 18 Schematic Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex Figure 6 16 PCB assembly MM 01 710 03S 01 ...

Page 149: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Schematic Diagrams 6 19 Figure 6 17 PCB assembly HEAT 01 710 04S 01 ...

Page 150: ...6 20 Schematic Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex Figure 6 18 PCB assembly MDCUI 02 Controller D05168 05 1 2 ...

Page 151: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Schematic Diagrams 6 21 Figure 6 19 PCB assembly MDCUI 02 Power D05168 05 2 2 ...

Page 152: ...6 22 Schematic Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex Figure 6 20 PCB assembly HM 01 710 06S 02 ...

Page 153: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Schematic Diagrams 6 23 Figure 6 21 RS Cable assembly 374 28X 01 ...

Page 154: ...6 24 Schematic Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 155: ...Abort page 7 5 7 1 11 2 BGN starts a sequence from memory page 7 5 7 1 11 3 Eak error Acknowledge page 7 6 7 1 11 4 Lop loop Command page 7 7 7 1 11 5 Moh move Head page 7 7 7 1 11 6 MOI move heater page 7 8 7 1 11 7 MOM move magnetic tips page 7 9 7 1 11 8 MOR rotate roundtable page 7 9 7 1 11 9 MOS move shield page 7 10 7 1 11 10 PAU pause for user intervention page 7 10 7 1 11 11 PAR set nonvol...

Page 156: ...7 2 Software Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 157: ...e example above the LF after the CR is an empty command 7 1 4 Response Format All response lines start with the same 3 character identifier as the command being executed The first response line contains only the command identifier and it is sent when the execution of the command starts The last response line contains the command identifier end field characters END and the uc_status field which is ...

Page 158: ... A quote character within the string must be expressed with two successive quotes It is perfectly valid to always enclose string arguments within quotes Example The user wants do display text My sequence in the lower display line To achieve this he she must send the command TXT My sequence 7 1 8 Recording Command Sequences The command set includes commands for recording received commands to the no...

Page 159: ...iolates these restrictions it will not be executed and an error is reported 7 1 11 Command Set Reference 7 1 11 1 Abort This command aborts execution of current command in the instrument The abort command is always processed before any other commands in the instrument s receive queue and it flushes the receive queue The head is driven up tip shield to pos shield the magnets are driven up heater is...

Page 160: ...n error 3 The turntable position is in error 4 Magnetic tips lift position is in error 5 Heater lift position is in error 6 The drop shield position is in error 7 Attempt to set the serial number when it already is set 8 Invalid command argument 9 Non volatile parameters lost 10 Attempt to set the IEEE address when it already is set 11 Internal software error This error is actually never reported ...

Page 161: ...nd arguments are allowed If uc_switch is 1 ul_time argument may be left out and then gets default value 0 Both ui_count and ul_time may not be 0 This command only works in sequences run from the nonvolatile memory It has no effect as a single command 7 1 11 5 Moh move Head This command moves the head to given y position The position is given in 1 10 mm with origo at the highest head position Posit...

Page 162: ...nates the delay and the command is completed Whenever the head is moved for some other reason than executing the MOH command the speed used is about 8 mm s Response MOH MOH END uc_status Recordable Yes Example MOH 500 1000 10 0 10 MOH MOH END 0 This command would move the head from current position to position 500 and back 10 times with one second delay between the cycles Speed is 100mm s is used ...

Page 163: ... s Range 3 200 The default after startup is 200 Only the ui_pos parameter is required Speed defaults to the last one used Response MOM MOM END uc_status Recordable Yes Example MOM 470 MOM MOM END 0 7 1 11 8 MOR rotate roundtable This command rotates the roundtable to given position The position is given as a value Command MOR si_pos ui_speed si_pos The number of the plate position turned under the...

Page 164: ...op speed in halfstep s Range 20 2000 The default after startup is 2000 Only the ui_pos parameter is required Speed defaults to the last one used Response MOS MOS END uc_status Recordable Yes Example MOS 1 MOS MOS END 0 7 1 11 10 PAU pause for user intervention This command starts a pause until the Start key is pressed Command PAU a_text a_text Text string to display See TXT command Response PAU PA...

Page 165: ... sensor offset Command PAR 1 si_rotate_offset si_magnet_offset si_head_offset si_heater_offset si_shield_offset si_rotate_offset Rotate stepper home sensor offset in steps si_magnet_offset Magnet stepper home sensor offset in steps si_head_offset Head stepper home sensor offset in steps si_heater_offset Heater stepper home sensor offset in steps si_shield_offset Shield stepper home sensor offset i...

Page 166: ... PID algorithm 0 100 si_offset Target temperature offset from the set temperature in 1 10 degrees Range 50 50 si_slope_start When the set temperature is above this value the target temperature is further adjusted by the slope value Range is from minimum set value to maximum set value 10 96 si_slope When the set temperature is above si_slope_start the target temperature is adjusted by si_slope degr...

Page 167: ...s The hardware address of the LAN interface in the following syntax XX XX XX XX XX XX where the X is one of characters 0 9 A F Only addresses assigned to Thermo Fisher Scientific are valid The address can only be set once 7 1 11 12 QIT ends the computer control This command ends the computer control Command QIT Response QIT QIT END uc_status Recordable No 7 1 11 13 REA read a byte or word value Th...

Page 168: ...nces can be run manually by first selecting the sequence with the arrow keys and then pressing Start key Maximum size of a protocol is 65000 bytes Format 1 Command REC uc_switch a_sequence a_name uc_switch 1 start recording a_sequence Name of the sequence max 17 characters a_name Sequence name already in memory The new sequence is linked to this sequence This format can be used for a new purificat...

Page 169: ...well Format 3 Command REC uc_switch uc_switch 0 stop recording This format is used for stopping the recording started with the format 2 command though it can also be used after format 1 command This command has no effect if recording is off Response REC REC END uc_status Recordable No REC 0 during recording stops the recording 7 1 11 15 REP report This command reports various instrument settings a...

Page 170: ...r Section 4 Memory usage Response REP 4 ul_total_memory ul_free_memory ul_largest_free_block ul_protocol_places ul_free_protocol_places a_protocol_names ul_total_memory Capacity of the protocol disk in bytes ul_free_memory Free memory space in bytes ul_largest_free_block Largest available memory block in bytes ul_protocol_places Number of protocol places ul_free_protocol_places Number of free prot...

Page 171: ...rval must be at least 100 Note that an argument error may be reported even if the ui_temp value is within the given range This may happen if the si_offset si_slope_start and si_slope parameters of PAR 4 command are such that the resulting target temperature is not within range 10 110 degrees Response TEM TEM ui_temp ui_ambient TEM END uc_status ui_temp Current heater temperature This may be text M...

Page 172: ... TEM 37 2500 TEM TEM 25 25 first value immediately TEM END 0 TEM 29 25 after 25 seconds 7 1 11 17 TIM timer display Display the given time minutes in the last two character positions of the upper display line The displayed time is decremented once a minute while a stored sequence is running If the sequence is paused the countdown is also paused The time display is cleared when the sequence stops T...

Page 173: ...bit the three screws from the part that connects the screw shaft to tip comb holder 5 When screws loosened Push the tip comb holder down and tighten the screws Make sure the tip comb holder goes up and down smoothly Clean and lubricate the shaft and rails if needed 6 N02023 head motor assembly is malfunctioning and needs to be replaced 3 Turntable pos error 1 Switch the instrument OFF and ON and t...

Page 174: ...track sensor assembly 3 Replace shield motor assembly N02024 7 Serial number set 1 Insert the serial number to instrument as described in section 9 2 If problem still continues replace the PCB assembly MAIN 02 7539 8 Invalid argument 1 Check for typing errors in commands and that not numeric values in commands are inside limits 2 If problem continues replace the PCB assembly MAIN 02 7539 9 Paramet...

Page 175: ...gnets 1 Check that the magnets are fully inserted 2 If magnets are in place but problem continues Replace the 2306000 micro switch cable assembly 1 15 Nonrecordable cmd 1 Check that no typing errors in commands Possible to make commands with Hwdoctor that are not recordable 2 If problem continues replace the PCB assembly MAIN 02 7539 16 Out of memory 1 Delete some unnecessary protocols from the in...

Page 176: ...7 22 Software Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 177: ... Comb Switch Test page 8 9 8 3 3 2 Magnet Assembly Switch Test page 8 9 8 3 3 3 Magnet Assembly Movement Test page 8 10 8 3 4 Turntable Offset Adjustment page 8 10 8 3 5 Head Offset Adjustment page 8 11 8 3 6 Turntable Straightness check page 8 11 8 3 7 Rot Test page 8 12 8 3 8 Head Test page 8 12 8 3 9 Tip Unload Test page 8 12 8 3 10 Tip Load Test page 8 12 8 3 11 Heating Plate Position Test and...

Page 178: ...8 2 Testing and Adjusting Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 179: ...o Section 8 3 2 on page 8 8 8 1 2 Magnet Head Check Use Flextest key in this test Make sure that tip comb and Deep well magnet head are in place Select m bottom the magnets should be as close as possible to the bottom of the tip comb Tip comb may move a little bit less than approx 0 5 mm when the magnets reach the bottom but not push out the tip comb Select m up When needed to adjust the magnet he...

Page 180: ... 1 6 Heating Assembly Check Use Flextest key in this test Make sure that tip comb and magnet head are in place Set KF96 plates to plate positions 1 to 8 Select heater up Check that KF96 plate is properly set to the changeable heating block The changeable heating block should rise smoothly inside the plate without scratching the plate nor lifting it from turn table Select heater down Select H align...

Page 181: ...rature 5 times with thermometer See Figure 8 1 on page 8 6 Measuring time is 10 seconds for each measuring point Measuring point is only in the middle of the plate Example VER VER KingFisher Flex 1 2 711 20 VER END 0 REP REP 0 711 20 REP END 0 REP Note Offset order in REP 1 REP 1 50 50 120 130 5 REP 1 50 50 120 130 5 REP END 0 Offset Rotate Magnet Head Heater Shield REP REP 2 KingFisher Flex REP E...

Page 182: ...urface Accepted temperature range is 80 1 C Select tem blow Heating block fan must start to blow Select Tem off After blower has stopped If there are any problems with heating block replace the heating block See section 12 Spare Parts 8 2 Functional Testing 8 2 1 Grounding Check Measuring with multimeter dead instrument Measuring point from middle pin of plug Maximum limit 0 3 Ohm Measuring points...

Page 183: ...OM cable to main ground screw Accepted limits for measuring values of power supplies 6 Switch off instrument 7 Set supply voltage to 256 V 8 Switch on instrument 9 Measure voltage from measuring points See Figure 8 2 on page 8 7 10 Switch off instrument Figure 8 2 Measuring points WARNING Be careful with supply voltage in terminal strips Power PD40 32LA Power SP 200 27 U DC V Min Max U DC V Min Ma...

Page 184: ...ttle when magnetic rods come down to bottom If magnetic rods stay too high select mag off down Select m up Select m bottom If magnetic rods stay too low select mag off up Select m up Select m bottom Repeat adjustment procedure until adjustment is ready 8 3 2 Lever Turner Adjustment Use Flexmagn key in this adjustment Select m load to drop tip comb Select m strip Loosen fixing screws of lever turne...

Page 185: ...k Repeat this procedure correspondingly to lever on otherside instrument Select m load and set tip comb in place Select m up and m bottom Magnet plate should move normally Figure 8 4 Testing tip comb switch 8 3 3 2 Magnet Assembly Switch Test Use Flextest key in this test Select tip unload to drop tip comb Remove magnet assembly Set tip comb back in place and select m up Select m bottom After this...

Page 186: ...y the plate hole check in what direction the turntable must be turned Select rot off ccw to turn turntable counterclockwise Select rot off cw to turn turntable clockwise Select h up Select pos 5 Select pos 1 Select h plate level and h alignment Repeat this procedure until adjustment is ready Select h up Set alignment plates or KF96 plates to positions 1 3 5 and 7 Select R alignment check Turntable...

Page 187: ... positions 3 5 7 similarly Select h plate level and h levelness Adjust tip level by changing value 610 using command moh 610 80 so that the lowest corner of tip level is as close as possible to alignment plate but not touching it to increase moh value tip level downwards to decrease moh value tip level upwards Check with feeler gauge that clearance from other corners to alignment plate is less tha...

Page 188: ...pos 4 Select pos 1 Select h plate level and h alignment Check that adjustment has not changed Select h up Remove alignment plate 8 3 8 Head Test Use Flextest key in this test Set KF96 plate to position 1 Select h test Test is passed only if you do not receive any error message Select h plate level Select h well bottom KF96 plate must not move but tip ends should stay as close as possible to KF96 p...

Page 189: ...le heating block with snap on connection Select Heater align up Check that tips of tip comb are in the middle of wells in interchangeable heating block Use only heating block for KingFisher 96 plates Figure 8 5 Loosening and tightening the fixing screws Select heater height down Select heater height up KF96 interchangeable heating block must not move but tips should be as close as possible to well...

Page 190: ...b is in place Select shield on Check shield straightness and adjust it when necessary Select shi off left or shi off right to adjust tip shield to center of tips Repeat adjustment when necessary Click twice shi off left Select shield off Select m bottom No error messages should be found Select m up Select twice shi off right Select shield off Select shield on Check adjustment is OK Select m bottom...

Page 191: ...tenance Procedures 9 1 Preventive Maintenance Check List page 9 3 9 2 Remove and Replace Belt on Turntable page 9 4 9 3 Preparing KingFisher Flex and PC for Functional Testing page 9 5 9 4 Instructions How to Program PCB Assembly MAIN 02 page 9 5 9 5 Entering Serial Number to Instrument with HWDoctor page 9 6 ...

Page 192: ...9 2 Maintenance Procedures Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 193: ...ment plate optional surface temperature reader A Cleaning and checkin g internal parts clean turntable and case clean spill shield clean IR readers clean check tightness of turntable tooth belts adjust if needed C DC volta ges measure voltages 5V 15V 27V see Service Manual section 8 pages 8 7 and 8 8 D Alignments using HW doctor tip load check see Service Manual section 8 magnet head check turntab...

Page 194: ...crews and lift the belt s away 3 Install new belt s so that opto plate and big track wheel are pointing to original directions If original positions are not marked opto plate is pointing to the same direction as opto sensor when the big wheel is positioned so that any position 1 8 of turntable is positioned roughly above the heating block 4 Tighten the belt with belt tensioner and fixing screws an...

Page 195: ...position Check that keyboard cable is connected Connect mains cable 9 4 Instructions How to Program PCB Assembly MAIN 02 Install new PCB assembly MAIN 02 Put a jumper to connector X25 of PCB assembly MAIN 02 See Figure 9 2 on page 9 5 Figure 9 2 Installing a jumper to connector X25 Install C167load exe program with all H86 files the latest C167 load program is available from MIB tech support group...

Page 196: ...g Serial Number and parameters to Instrument with HWDoctor Write par 0 711 xxxxx serial number and press ENTER Write Rep 0 and check serial number is correct Parameters can be loaded as described in chapter 7 1 11 11 If original instrument specific parameters are not known contact MIB Technical Support team in Finland via services mi fi thermofisher com to receive the original factory settings Swi...

Page 197: ...y 10 1 Section 10 Power Supply Name Type Page Module PD40 32LA DC DC power supply 10 30V 30W B127W76 2H34 See Figure 10 1 on page 10 3 See Figure 10 2 on page 10 4 Module SP 200 27 AC DC power supply 85 264V 200W B199W99H50 See Figure 10 3 on page 10 5 See Figure 10 4 on page 10 6 ...

Page 198: ...10 2 Power Supply Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 199: ...angsdaten Input Specifications Eingangsspannung Input voltages 10 30 L 20 60 M 30 90 H VDC Ausgangsdaten Output Specifications Leistung Power 40 Watt Wirkungsgrad Efficiency typ 75 Schaltfrequenz Switching frequency 36 kHz Regelabweichung Regulation Eingang Line 0 5 max at full load Hauptausgang Main output 3 Kreuzregelung Cross 5 Ausregelzeit 1 Recovery time 1 5 Ps 25 load step Restwelligkeit Rip...

Page 200: ...it einer relativen Dauer von weniger als 10 3 Restwelligkeit bei 20 MHz Bandbreite unter Verwendung einer 10 μF Kapazität parallel zu einem 0 1 μF Kera mikkondensator sowie einer Spule mit 10 Windungen gemessen Bestellbeispiel PD40 31 MC M Eingangsspannung C Mechanik Remarks 1 The maximum continuous output power must not exceed 40 W with convection cooling the L series not 30 W 2 Peak current last...

Page 201: ...es Universal AC input full range Built in active PFC function PF 0 93 Protections Short circuit Over load Over voltage Over temperature Built in cooling fan speed control Built in constant current limiting circuit Built in fan speed control Remote ON OFF control Optional LED indicator for power on 100 full load burn in test Fixed switching frequency at PFC 67KHz PWM 134KHz 3 years warranty Feature...

Page 202: ...umber assignment Pin No Pin No 1 4 5 2 3 6 7 Assignment Assignment AC L AC N FG DC OUTPUT V DC OUTPUT V 10 20 30 40 50 60 HORIZONTAL 20 40 60 80 100 10 0 Remote ON OFF CN1 JST S2B XH or equivalent optional Pin No 1 2 Assignment Mating Housing JST XHP or equivalent Terminal JST SXH 001T P0 6 or equivalent RC RC 90 100 80 70 60 50 40 142 117 65 25 70 35 20 25 15 16 199 5 M3 L 3mm 8 1 2 3 R C V ADJ 9...

Page 203: ...wer jumper cable assembly N01109X Rev 2 See Figure 11 9 on page 11 7 24V power cable assembly N01110X Rev 2 See Figure 11 10 on page 11 7 Power cable 2 assembly N01111X Rev 2 See Figure 11 11 on page 11 8 Power GND cable assembly N01112X Rev 2 See Figure 11 12 on page 11 8 RS cable assembly 374 28X 02 See Figure 11 13 on page 11 8 Main GND cable assembly 1255 02X 01 See Figure 11 14 on page 11 9 P...

Page 204: ...11 2 Wiring Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 205: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Wiring Diagrams 11 3 Figure 11 1 Switch cable 2306000 Figure 11 2 Switch cable 2 2306010 710 01X 01 Bending 30 length 3 mm 710 02X 02 ...

Page 206: ... Manual Thermo Fisher Scientific KingFisher Flex Figure 11 3 Magnet lift cable assembly 1295280 Figure 11 4 Magnet motor assembly 2306020 Cable total length 340 mm 710 03X 02 710 04X 01 Wires cut 90 mm twisted pairs BLACK RED BLUE GREEN 80 mm ...

Page 207: ... Flex Thermo Fisher Scientific Service Manual Version 1 1 Wiring Diagrams 11 5 Figure 11 5 HM folio cable 1295290 Figure 11 6 HEAT folio cable 1295300 710 05X 02 Cable length 400 mm 710 06X 01 Cable length 265 mm ...

Page 208: ... 1 Service Manual Thermo Fisher Scientific KingFisher Flex Figure 11 7 Mains filter switch cable assembly N01107 Figure 11 8 Switch power cable assembly N01108 N01107 02X BLUE 120 mm BROWN 120 mm N01108 02X BLUE 240 mm BROWN 240 mm ...

Page 209: ...Scientific Service Manual Version 1 1 Wiring Diagrams 11 7 Figure 11 9 Power jumper cable assembly N01109 Figure 11 10 24V power cable assembly N01110 N01109 02X BLUE 230 mm BROWN 230 mm N01110 02X RED 70 mm BLACK 240 mm RED 150 mm ...

Page 210: ...ure 11 11 Power cable 2 assembly N01111 Figure 11 12 Power GND cable assembly N01112 Figure 11 13 RS cable assembly 2305190 N01111 02X BROWN GREEN BLUE WHITE GRAY N01112 01X 374 28X 02 1 X 1 X 2 1 5 2 0 2 5 5 1 0 4 0 0 m m O R B R R E O R 4 2 0 m m R E 4 2 0 m m B R 4 2 0 m m 7 3 2 1 a 1 0 m m ...

Page 211: ...isher Flex Thermo Fisher Scientific Service Manual Version 1 1 Wiring Diagrams 11 9 Figure 11 14 Main GND cable assembly 2305600 Figure 11 15 Power CHAMP 8 cable 2305950 1255 02X 01 3001 19X 03 Length 300 mm ...

Page 212: ...10 Wiring Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex Figure 11 16 Power 24V cable assembly Figure 11 17 26V power jumper cable N03440 3001 20X 02 3001 40X 01 Length 300 mm ...

Page 213: ...r Scientific Service Manual Version 1 1 Wiring Diagrams 11 11 Figure 11 18 Fan assembly 2103900 Figure 11 19 Head motor assembly N02023 347 06X 01 N02023 01X WHITE position 5 GREEN position 6 BLACK position 4 RED WHITE position 3 ...

Page 214: ...isher Flex Figure 11 20 Track sensor assembly 2305200 374 29X 02 1 2 3 4 G R V H W H V A R E P U B A M U V H V A P U M U E C 2 0 X 1 1 0 V 1 J o h d o t k i e r r e t ä ä n p a r e i t t a i n k o k o p i t u u d e l t a 2 6 0 m m W i r e s c u t 2 7 0 m m t w i s t e d p a i r s ...

Page 215: ...on 1 1 Wiring Diagrams 11 13 Figure 11 21 Heating block shield motor N02024 Soldering RED position 1 BLUE position 2 YELLOW position 4 GREEN position 3 YELLOW position 4 RED position 1 GREEN position B BLUE position A 350 mm 350 mm 350 mm 350 mm 330 mm N02024 01X ...

Page 216: ...11 14 Wiring Diagrams Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

Page 217: ...y on page 12 8 see Lifting Mechanics on page 12 5 see Rotary Table Assembly Turntable Assembly on page 12 10 N07941 Heater resistor plate assembly see Heater Assembly on page 12 9 21040990 Stepper motor assembly see Heater Assembly on page 12 9 1130870 Return spring see Heater Assembly on page 12 9 N02024 Shield motor assembly see Tip Shield Assembly on page 12 8 N02184 Spring see Tip Shield Assem...

Page 218: ...o Fisher Scientific KingFisher Flex SP 00142 KF Flex service alignment head Special DW magnet head for cases when DW head not available from customer SP 00139 KF Flex 96 alignment kit Incl alignment plate and consumables demo kit CODE DESCRIPTION PAGE ...

Page 219: ...able disc assembly Refer to section 12 2 on page 12 4 Lifting mechanics Refer to section 12 3 on page 12 5 Refer to section 12 4 on page 12 6 Refer to section 12 5 on page 12 7 Refer to section 12 6 on page 12 8 Heater assembly Refer to section 12 7 on page 12 9 Rotary table assembly Refer to section 12 8 on page 12 10 Mask assembly incl PCB assembly MDCUI 02 LCD display and keyboard Power supplie...

Page 220: ...ersion 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex 12 2 Rotary Table Disc Assembly ROTARY TABLE DISC ASSEMBLY N07905 Position Item Cat no 1 Rotary table disc N07901 5 Rotary table spring N07902 D06426X 01 ...

Page 221: ...3 Lifting Mechanics LIFTING MECHANICS Position Item Cat no 1 Bearing 1143200 2 Linear guide 1190980 3 Track sensor assembly 2305200 4 KF96 Micro switch cable assembly 1 2306000 5 Head motor assembly N02023 6 Tip comb holder Refer to section 12 4 on page 12 6 21041490 1 2 3 4 5 6 D06427X 01 ...

Page 222: ... Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex 12 4 Tip Comb Holder TIP COMB HOLDER Position Item Cat no 1 Magnet motor assembly 2306020 2 KF96 Micro switch cable assembly 2 2306010 1 2 710 04X 01 ...

Page 223: ...hermo Fisher Scientific Service Manual Version 1 1 Spare Parts 12 7 12 5 Mounting Plate Assembly MOUNTING PLATE ASSEMBLY Position Item Cat no 10 PCB assembly KF MM 01 2306190 25 KF96 magnet lift cable 1295280 D06428X 02 ...

Page 224: ...1 Service Manual Thermo Fisher Scientific KingFisher Flex 12 6 Tip Shield Assembly TIP SHIELD ASSEMBLY 21041480 Position Item Cat no 1 Track sensor assembly 2305200 2 Shield motor assembly N02024 3 Spring N02184 2 1 710 03X 01 3 ...

Page 225: ...EMBLY Position Item Cat no 1 HM cable from PCB assembly MAIN 02 to PCB assembly HM 01 1295290 2 Heat cable from PCB assembly MAIN 02 to PCB assembly HEAT 01 1295300 3 Track sensor assembly 2305200 4 Heater resistor plate assembly N07941 5 Stepper motor assembly 21040990 6 Return spring 1130870 1 2 3 4 5 6 D06418X 01 ...

Page 226: ...isher Scientific KingFisher Flex 12 8 Rotary Table Assembly Turntable Assembly ROTARY TABLE ASSEMBLY TURNTABLE ASSEMBLY Position Item Cat no 1 Track sensor assembly 2305200 2 Tooth belt 2 pcs N07937 3 Rotary table motor assembly N07952 1 2 2 3 D06424X 02 ...

Page 227: ...KingFisher Flex Thermo Fisher Scientific Service Manual Version 1 1 Bulletins 13 1 Section 13 Bulletins Document no Title N A ...

Page 228: ...13 2 Bulletins Version 1 1 Service Manual Thermo Fisher Scientific KingFisher Flex ...

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