6. Operating the infinite® 200
2008-07
Instructions for Use for
infinite® 200
No. 30017581 Rev. No. 1.4
99
6.3.3
Measurement Accessories
Recommended Filters (
infinite
®
F200
Only)
Please ask your local Tecan dealer for a recommended filter set. Filters designed
for a different type of instrument will not necessarily perform well with the
infinite
®
F200
.
Note
If the excitation and the emission maximum of a fluorescent species are
close together, they should not be directly translated into center
wavelengths for fluorescence filters.
To provide acceptable background, usually, the upper cutoff for excitation
wavelengths on the one hand, and the lower cutoff for emission wavelengths on
the other hand need to be separated. This compromise depends on the blocking
properties of the filters. For many fluorescent molecules signal may be improved
by expanding filter bandwidth away from the other band pass, respectively.
Recommended Types of Microplates
Generally, for high fluorescence sensitivity, black microplates are recommended.
For low concentrations of TRF labels, white microplates seem superior. You may
check if white plates are superior with UV excitation wavelengths.
We do not recommend using volumes less than a third of the maximum volume.
When using lower volumes, check the availability of a suitable plate type.
In order to ensure good performance for Fluorescence Bottom Reading, we
recommend using black plates with transparent bottom.
All standard microplates from 6 to 384 wells (maximum plate height 23 mm
including lid) that conform to the following standards can be measured:
ANSI/SBS 1-2004, ANSI/SBS 2-2004; ANSI/SBS 3-2004 and ANSI/SBS 4-2004.
When installing the operating software (i-Control or Magellan), pre-defined plate
definition files are installed. Please refer to the following list for the corresponding
ordering numbers of the microplates. Please order microplates at your local
microplate supplier.
Plate Definition File
(*.pdfx)
Catalog Number
Manufacturer
GRE6ft
657 160
657 185
Greiner Bio-One,
www.gbo.com/bioscience
GRE12ft
665 180
665 102
Greiner Bio-One,
www.gbo.com/bioscience
GRE24ft
662 160
662 102
Greiner Bio-One,
www.gbo.com/bioscience
GRE48ft
677 180
677 102
Greiner Bio-One,
www.gbo.com/bioscience
GRE96ft
655 101
655 161
Greiner Bio-One,
www.gbo.com/bioscience
GRE96fb_chimney
655 079
655 086
655 077 (Fluotrac 600)
655 076 (Fluotrac 200)
Greiner Bio-One,
www.gbo.com/bioscience
GRE96fw_chimney
655 073
655 083
655 074 (Lumitrac 600)
655 075 (Lumitrac 200)
Greiner Bio-One,
www.gbo.com/bioscience
