5. Optical System
78
Instructions for Use for
infinite® 200
No. 30017581 Rev. No. 1.4
2008-07
5.5 Cuvette Port (
infinite
®
M200
)
The
infinite
®
M200
may be optionally equipped with a cuvette port for
absorbance measurements.
Figure 5-7: Optical System of the absorbance module of
infinite
®
M200
including
the cuvette port
For absorbance measurements with the cuvette port of
infinite
®
M200
a similar
optical path is used as for fluorescence excitation. For details of the light source
(1) and the excitation monochromator (2), please refer to chapter
2.2.1 Fluorescence/A) Fluorescence Intensity (FI). A fiber bundle guides the light
from the excitation monochromator to the absorbance cuvette optics (3), which
focuses the light through the cuvette. The absorbance cuvette measurement
module (4) is located right after the cuvette port. A silicon photo diode measures
the light being transmitted through the sample. Before measurement of the
cuvette, a reference measurement against air is performed with the cuvette port
moved out of the light beam. The figure also shows the light path of the
absorbance microplate module (5).
1
2
3
4
5
