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8.
Quality
Control
2008-07
Instructions for Use for
infinite® 200
No. 30017581 Rev. No. 1.4
141
Calculation of the Sensitivity (Detection Limit):
15
B
ATP
B
8
e
1
1
*
0002
.
0
*
mean
mean
Stdev
*
3
*
10
2
)
well
/
fmol
(
imit
DetectionL
−
−
−
⋅
=
2*10
-8
Concentration of ATP standard [M]
Stdev
B
Standard deviation of Blank
mean
ATP
Average of wells filled with ATP standard
mean
B
Average of Blank wells
0.0002
Conversion into mol/well
1/1e
-15
Conversion into fmol/well
The result of the formula determines the detection limit in fmol/well.
8.3.6
Absorbance Accuracy
Use MultiCheck Plate – For details please refer to the MultiCheck Instructions for
Use.