6. Operating the infinite® 200
104
Instructions for Use for
infinite® 200
No. 30017581 Rev. No. 1.4
2008-07
6.4.5
Measurement with a Simultaneous G-Factor Calibration
When
Calibrate
is selected, the G-factor is determined for the current
measurement parameters and used for the following FP measurement. In order
to perform the G-Factor calibration, please define:
•
Reference value
: select a polarization value for the reference used, e.g.
20 mP for a 1 nm fluorescein solution in 0.01 M NaOH.
•
Reference range
: click
Change
and select the wells filled with the
reference.
•
Blank range
: click
Change
and select the wells filled with the reference
blank. Select
Same as measurement blank
if the reference blank is the
same as the measurement blank.
Note
By filling in more than one well with polarization references and
reference blanks, the mean values will be calculated and therefore the
calibration result will be more accurate.
(Translation for German User)
Anmerkung:
Bei Verwendung von mehr als einem ‚Well’ für die Positionen
‚Polarization Reference’ und ‚Polarization Blank’, werden für die
Kalibration und für die weiteren Berechnungen die Mittelwerte
herangezogen. Die Kalibration wird duch Mehrfachbestimmung genauer.
G-Factor Storage
The calculated G-Factor is automatically stored on the computer’s hard drive.
Each G-Factor entry corresponds to the filter pair selection as well as the filter
slide description. There is always only one G-Factor available for the respective
filter pair combination and filter slide description, unless the same filter pair has
been used with the different filter slides and thus stored with the different filter
slide descriptions.
