2.
General
Description
2008-07
Instructions for Use for
infinite® 200
No. 30017581 Rev. No. 1.4
29
Reagent Backflush
Reagent backflush allows reagents in the tubing system to be pumped back into
storage bottles. This action can be performed optionally prior to washing the
injector system to minimize the dead volume.
Reagent backflush procedure:
1. Remove the injector carrier from the instrument and insert the injector
carrier into the service position of the injector box.
2. Insert the feeding tubing into the appropriate storage bottle.
3. Adjust parameters on the
Backflush
tab of the
Injector Maintenance
dialog box in the
Settings
menu
4. Start the reagent backflush procedure by clicking
Start backflush
.
Backflush
Select one of the injectors
Injector A
or
Injector B or Injector A
and B
(only ‘primed’ injectors are available for ‘backflush’).
Select the
Piston Strokes
(1 – 60; 1 stroke equals 1 ml)
Select the
Backflush Speed
(100 - 300 µl/sec).
Select the ‘
Refill Speed
’ (100 – 300 µl/sec.) or select the
Refill
Speed equal to Backflush Speed
check box.
Click
Start backflush
to start the reagent backflush procedure.
Click
Close
to exit the dialog box.
