tecan infinite 200 Instructions For Use Manual Download Page 75

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5. Optical System

2008-07

Instructions for Use for

infinite® 200

No. 30017581 Rev. No. 1.4

5.3 Fluorescence Polarization System

―

infinite

F200

For technical details please refer to chapter 5.2 Fluorescence Intensity System

―

infinite

F200

infinite

F200

configured for Fluorescence Polarization (FP) measurements is

delivered with a standard FP filter slide. The filter slide is equipped with filters and
polarizers for excitation and emission, at 485 and 535 nm respectively, and can
be applied for measuring, for example, fluorescein-based FP applications.

For details on how to mount polarizers and FP filters please refer to
chapter 4 Defining Filter Slides (

infinite

F200

only).

Summary of Contents for infinite 200

Page 1: ...TECAN Instructions for Use for infinite 200 Document Part No 30017581 2008 07 Document Revision No 1 4...

Page 2: ...te any comments on this publication Manufacturer Tecan Austria GmbH Untersbergstrasse 1A A 5082 Gr dig Salzburg AUSTRIA EUROPE T 43 62 46 89 33 F 43 62 46 72 770 E mail office austria tecan com www te...

Page 3: ...respective owners i ControlTM magellanTM infinite Tecan and the Tecan Logo are registered trademarks of Tecan Group Ltd M nnedorf Switzerland Windows and Excel are registered trademarks of Microsoft...

Page 4: ...CATES THE POSSIBLE PRESENCE OF FLAMMABLE MATERIALS AND A RISK OF FIRE PROPER LABORATORY SAFETY PRECAUTIONS MUST BE OBSERVED ATTENTION NEGATIVE ENVIRONMENTAL IMPACTS ASSOCIATED WITH THE TREATMENT OF WA...

Page 5: ...Injector Injector Carrier 22 2 3 4 Priming and Washing of the infinite 200 25 2 3 5 Injector Cleaning and Maintenance 35 2 3 6 Injector Reagent Compatibility 36 2 4 Software 38 2 4 1 i Control and Inj...

Page 6: ...tion 74 5 3 Fluorescence Polarization System infinite F200 75 5 4 Absorbance System infinite M200 76 5 4 1 Absorbance Optics MTP 77 5 4 2 Absorbance Detection MTP 77 5 5 Cuvette Port infinite M200 78...

Page 7: ...MRW Type 109 6 6 2 MRW Size 110 6 6 3 MRW Border 110 6 6 4 Result Display in MS Excel 112 6 6 5 Miscellaneous Software Features of MRW 112 6 7 Optimize Luminescence Measurements 113 6 7 1 Integration...

Page 8: ...ce Accuracy 141 8 3 7 Absorbance Wavelength Accuracy 142 8 3 8 Absorbance Baseline Flatness infinite M200 143 8 3 9 Absorbance Baseline Flatness infinite F200 144 8 3 10 Absorbance Cuvette infinite M2...

Page 9: ...g the stored Plate Definition Files that are received with the instrument software We take every precaution to ensure that the plate heights and well depths are correct according to the defined plate...

Page 10: ...owing laws and guidelines 1 National industrial protection law 2 Accident prevention regulations 3 Safety data sheets of the reagent manufacturers WARNING DEPENDING ON THE APPLICATIONS PARTS OF THE in...

Page 11: ...ic compatible 2 1 1 Intended Use The infinite 200 has been designed as a general purpose laboratory instrument for professional use supporting common 6 to 384 well microplates conforming to the ANSI S...

Page 12: ...e following measurement techniques Fluorescence Intensity FI Top Fluorescence Intensity FI Bottom Fluorescence Time Resolved TRF Flash Fluorescence Fluorescence Polarization FP Absorbance Absorbance w...

Page 13: ...fluorescence intensity and absorbance measurements Via software any wavelength can be easily adjusted within the specified wavelength range In addition to single wavelength measurements absorbance an...

Page 14: ...D ROM Drive Screen Resolution 1024 x 768 Recommended Pentium P4 2 GHz 40 GB HDD 512 MB RAM 2 x USB 2 0 1 x RS232 CD ROM Drive Screen Resolution 1280 x 1024 Operating System Requirements Windows XP Pro...

Page 15: ...Fluorescence An Abstract Fluorescent molecules emit light of specific wavelength when struck by light of shorter wavelength Stokes Shift In particular a single fluorescent molecule can contribute one...

Page 16: ...T based applications by Brian A Pollok and Roger Heim trends in Cell Biology Vol 9 February 1999 Overview is given in the Review Article Application of Fluorescence Resonance Energy Transfer in the Cl...

Page 17: ...he slow rotation of the labeled molecule indicating that binding probably did occur A resultant low mP value denotes a fast rotation of a molecule indicating that binding probably did not occur The FP...

Page 18: ...ing example Bioluminescence Methods and Protocols ed R A LaRossa Methods in Molecular Biology 102 Humana Press 1998 Bioluminescence Resonance Energy Transfer BRET TM BRET TM is an advanced non destruc...

Page 19: ...minescence is one of the measurement modes that can be performed with injectors Note The plate detection sensor is only active if one of the injectors is in use strips injection or dispense Note Durin...

Page 20: ...c view of the injector module There are up to two pumps available for the infinite 200 see Figure 2 1 above Pump A feeds injector needle A Pump B feeds injector needle B The infinite 200 can be equipp...

Page 21: ...ime delay approx 0 5 s between injection and reading occurs For details on how to set up a measurement with injectors please refer to chapter 2 4 1 i Control and Injectors 2 3 2 Storage Bottles The in...

Page 22: ...emove the injector dummy and insert the carrier into the injector port Press the carrier softly into the injector port until you hear a clicking noise The instrument contains an injector sensor that c...

Page 23: ...e injector carrier must be in the service position for washing und priming Prime and Wash must not be performed when the injector is in the instrument STOP Caution If the injector carrier is not inser...

Page 24: ...Figure 2 4 Injector box with injector in service position Before starting a measurement make sure that The tubes are clean If not please refer to chapter 2 3 4 Priming and Washing of the infinite 200...

Page 25: ...priming and washing steps of the injector system a default setting for injection speed and volume dispensed is provided If required the priming parameters can be adjusted in the injector control wind...

Page 26: ...rom the carrier slot and inserted into the holder of the injector carrier system STOP Caution Do not touch the injector needles They can become easily bent or misaligned which can cause injection prob...

Page 27: ...container under the injector Priming procedure i Control 1 Adjust parameters at the prime tab of the injector maintenance dialog box in the settings menu 2 Activate the priming procedure by clicking t...

Page 28: ...pense conditions are equal for each well STOP Caution Close the lid of the pump module injector box completely before starting a measurement Priming Example Operational Sequence of Pump System The fol...

Page 29: ...box 2 Insert the feeding tubing into the appropriate storage bottle 3 Adjust parameters on the Backflush tab of the Injector Maintenance dialog box in the Settings menu 4 Start the reagent backflush...

Page 30: ...a wash procedure to clean the injector system Wash procedure Washing can be performed by using the i Control software or by using the hardware buttons on the injector box Washing general procedure 1...

Page 31: ...Settings menu 2 Start the washing procedure by clicking the Start wash Wash Select one of the injectors Injector A or Injector B or Injector A and B Select the Piston Strokes 1 60 1 stroke equals 1 ml...

Page 32: ...n The injector carrier must be in the service position for the action wash Do not perform washing when the injector is in the instrument STOP Important Be sure to run a final wash procedure with disti...

Page 33: ...depends on the selected refill mode selected on the injector or dispense strip see chapter 2 4 1 i Control and Injectors for details When using standard refill mode the dispense step is performed aft...

Page 34: ...tion Place the waste tub on the plate transport before starting a measurement with the actions injection and or dispense STOP Caution It is recommended to empty the waste tub before starting a measure...

Page 35: ...s Weekly Periodical Maintenance The injector system tubing syringes injector needles must be cleaned weekly to remove precipitates and eliminate bacterial growth Follow these steps to clean the pump i...

Page 36: ...ompatibility with the injector system Chemicals with a rating D must not be used with the infinite injectors They will severely damage the injector system Chemical Rating Acetic Acid 60 A Acetonytrile...

Page 37: ...opriate chemical compatibility Before permanent installation test the equipment with the chemicals and under the specific conditions of your application STOP Caution Variations in chemical behaviour d...

Page 38: ...available Dispense The dispense mode allows liquid to be dispensed plate wise into the selected wells Injection This mode must be used in combination with a measurement strip The injection is performe...

Page 39: ...nse strip does not require an additional measurement strip Dispense volume The injection volume depends on the microplate type The plate definition files include a so called working volume This workin...

Page 40: ...t refill mode Standard if refill should be performed when syringe is empty multiple injection steps are performed before refilling refill occurs after dispensing approx 800 l Select Refill for every i...

Page 41: ...injection parameters Only one injector can be selected per strip If both injectors are required or one injector will perform two injections an additional injection strip has to be inserted Measuremen...

Page 42: ...ip A Wait time delay or settle time can be inserted into the procedure Wait time Select a time in hh mm ss from 00 00 01 up to 23 59 59 Options If Wait for injection is selected the wait time includes...

Page 43: ...of the plate Not shown can be optionally selected if only part of the plate is to be processed Well Mandatory for measurements with injection Injection 1 Injector A injects 100 l with speed 200 l sec...

Page 44: ...measurements white microplates are recommended For this example a white 96 well plate was selected Part of the plate Not shown can be optionally selected if only part of the plate should be processed...

Page 45: ...ws actions to be performed once in a kinetic run at a certain cycle The intended injection strip below it is only processed once at the selected cycle Injection Injector A injects 20 l with speed 200...

Page 46: ...ws actions to be performed once in a kinetic run at a certain cycle The intended injection strip below it is only processed once at the selected cycle Injection Injector A injects 20 l with speed 200...

Page 47: ...ludes additionally the following items Accessory Box Filter stop rings 8 Filter assembly tool Plastic tweezers Filter slide The injector module packaging for 1 injector includes the following items Bo...

Page 48: ...e that it is held on both sides 5 Visually inspect the instrument for loose bent or broken parts Report any damage immediately 6 Compare the serial number on the rear panel of the instrument with the...

Page 49: ...ier locked into place so that they cannot be damaged Before the instrument can be used the transport locks must be removed using the following procedure 1 Ensure that the instrument is disconnected fr...

Page 50: ...emove the screw from the transport lock which is only in the plate carrier compartment right transport lock in picture above 7 Slide the transport locks off of the pins and remove them from the plate...

Page 51: ...rature 20 C to 60 C 4 F to 140 F Relative Humidity 90 non condensing 3 4 Power Requirements The instrument is auto sensing and it is therefore not necessary to make any changes to the voltage range Ch...

Page 52: ...ent is in the OFF position 2 Connect the computer to the instrument with the delivered USB interface cable 3 Insert the power cable into the main power socket with protective ground connection on the...

Page 53: ...E AC 100 120 220 240 50 60 1 Instrument Fan 2 Power Supply Fan 3 Main Power Switch 4 Main Power Socket 5 Label Technical Inspection Agency 6 Label Options Configuration 7 Name Plate 8 Warranty Label 9...

Page 54: ......

Page 55: ...monly used in microplate readers for absorbance measurements usually have a bandwidth of 10 nm Therefore it is not recommended to use fluorescence filters for absorbance measurements because the bandw...

Page 56: ...fore inserting a new filter carefully consider the filter and the direction of light through the filter slide 1 Filters with an arrow on the side Light must travel in the direction of the arrow 2 Filt...

Page 57: ...ether with the polarizers either on the positions 1 and 2 or 3 and 4 The infinite F200 filter slide can be equipped with maximal two different fluorescence polarization filter pairs as each fluorescen...

Page 58: ...stop ring by pulling it out of the filter slot 2 The filter will slide out of the filter slot when the filter carrier is turned over Do not use the filter assembly tool to remove filters 4 3 2 Mountin...

Page 59: ...er slide using tweezers taking care not to scratch it or get fingerprints on it 2 Carefully insert the filter into the opening taking care not to scratch or get fingerprints on the filter 3 Place the...

Page 60: ...engineer or if a new undefined customized filter slide is to be used the filter slides need to be defined Depending on the frequency of use and environmental conditions optical filters may deteriorat...

Page 61: ...r key value If manually entered avoid using the same description for the different filter slides Position 1 4 Filter definition editor for the filters filter pairs on positions 1 2 3 and 4 Select the...

Page 62: ...velength within the following range 1 Fluorescence intensity mode 230 to 850 nm Excitation and 280 to 850 nm Emission 2 Fluorescence polarization 300 to 850 nm Excitation and 330 to 850 nm Emission 3...

Page 63: ...er number is saved together with other information about the filter on the filter slide microchip If you replace a filter this information will be lost unless the last filter flash number is manually...

Page 64: ......

Page 65: ...ding the excitation double monochromator 2 the fluorescence top optics 3 the emission double monochromator 4 and the fluorescence detection 5 The solid arrows indicate the light path of the excitation...

Page 66: ...ystem 66 Instructions for Use for infinite 200 No 30017581 Rev No 1 4 2008 07 Figure 5 2 Optical System Fluorescence Bottom Figure 5 3 Detailed view of excitation and emission double monochromator uni...

Page 67: ...time 3 Many applications only one kind of lamp 4 No warm up time required Condenser Condenser type optics from fused silica focus the flash light onto the entrance slit of the excitation monochromator...

Page 68: ...en the monochromator is set for light with a wavelength of 500 nm and the detector detects a signal of 10 000 counts light with different wavelengths creates a signal of only 10 counts For application...

Page 69: ...light source The lens system at the end of the excitation top fiber is designed to focus the excitation light into the sample and also collect the fluorescence light and focus it back onto the fluores...

Page 70: ...ate scatter of excitation light and nonspecific fluorescence The wavelength range is selectable from 330 600 nm in the standard instrument and from 280 850 nm in the spectrally enhanced instrument The...

Page 71: ...ce intensity system of the infinite F200 instrument Light Source 1 Fluorescence Optics 2 Fluorescence Detection System 3 The fluorescence top system is shown in Figure 5 4 the bottom system in Figure...

Page 72: ...5 Optical System 72 Instructions for Use for infinite 200 No 30017581 Rev No 1 4 2008 07 Figure 5 5 Fluorescence intensity bottom system of the infinite F200 1 2 2 2 3...

Page 73: ...ind of lamp are 1 High intensity from the deep UV to the near IR 2 Very long lifetime 3 Many applications only one kind of lamp 4 No warm up time required Condenser Condenser type optics focuses the l...

Page 74: ...ial provides high UV transmission and is virtually void of auto fluorescence 5 2 3 Fluorescence Optics Bottom Flash light enters the optical system by being focused through an orifice This opening act...

Page 75: ...n infinite F200 configured for Fluorescence Polarization FP measurements is delivered with a standard FP filter slide The filter slide is equipped with filters and polarizers for excitation and emissi...

Page 76: ...urce System Fluorescence Intensity A fiber bundle guides the light from the excitation monochromator to the absorbance microplate MTP optics 3 which focuses the light into the wells The absorbance MTP...

Page 77: ...beam is 0 7 mm in diameter 5 4 2 Absorbance Detection MTP A silicon photodiode is used for the measurement of the transmitted light It is sensitive to a wide range of wavelengths The photodiode is wel...

Page 78: ...the light source 1 and the excitation monochromator 2 please refer to chapter 2 2 1 Fluorescence A Fluorescence Intensity FI A fiber bundle guides the light from the excitation monochromator to the a...

Page 79: ...from the excitation monochromator system to the absorbance cuvette optics This optics consists of a pair of lenses focusing the light beam into the cuvette At the focal point the spot diameter of the...

Page 80: ...mm 0 5 ml Hellma 104 002 QS 10 mm Ultra micro cuvettes 12 5 x 12 5 mm 55 mm 100 l Hellma 105 202 10 mm Cuvettes with a measurement window 2 mm diameter cannot be used STOP Caution Always use a valid f...

Page 81: ...moves the cuvette in and out The cuvette carrier is an integral part of the instrument and cannot be removed Figure 5 8 Cuvette Port infinite M200 The cuvette has to be inserted so that the measuremen...

Page 82: ...ette out and in button Blanking The software allows a so called Blanking measurement Blanking in the Instrument menu is only available when a measurement script containing a cuvette measurement is ope...

Page 83: ...another blanking measurement Blanking data will be deleted without a warning message when closing the i Control software 5 5 6 i Control Cuvette Examples Example 1 Example of how to use the Blanking...

Page 84: ...blank cuvette Prepare sample cuvette and put it on the cuvette holder Start the measurement by clicking Start 6 The cuvette holder is moved in and the measurement is performed The measured data Value...

Page 85: ...fixed wavelength strip Apply blanking is disabled when a reference wavelength is selected Select the appropriate measurement parameters wavelength number of flashes and settle time Plate Necessary fo...

Page 86: ...the measurement and therefore allows removing the cuvette from the cuvette port When confirming the request the measurement continues Move Cuvette In The cuvette port is moved in Plate Necessary for m...

Page 87: ...cated underneath the plate carrier It measures the light being transmitted through the sample Before measurement of the microplate a reference measurement is performed with the plate carrier moved out...

Page 88: ...escence excitation Light focused through the dispensed liquid is slightly refracted at the interfaces between air liquid and plate bottom To accomplish a reliable measurement in the presence of the me...

Page 89: ...e detection unit PMT passing a filter wheel The PMT photomultiplier tube is designed for applications in chemo and bioluminescence providing a high dynamic range The exceptional low noise and high sen...

Page 90: ...heel with 6 filter positions in front of the PMT window is switched to the required luminescence channel The sensitivity of the detection system makes it necessary to attenuate high luminescence light...

Page 91: ...pectrum of filter Lumi Green 0 10 20 30 40 50 60 70 80 90 100 400 450 500 550 600 650 700 750 Wavelength nm Transmission Figure 5 16 Transmission spectrum of filter Blue 1 0 10 20 30 40 50 60 70 80 90...

Page 92: ...s is based on a dedicated luminescence PMT with appropriate measurement circuitry This technique is very robust against noise It is preferred for measurement of very low light levels For best performa...

Page 93: ...tant exception STOP Caution When placing a microplate into the plate carrier always make sure that the correct plate definition file plate height has been selected in the software before you do anythi...

Page 94: ...performed Initialization of OS filter wheels M200 only Initialization of luminescence filter wheel Initialization of z transport of luminescence optics Initialization of plate transport The plate tran...

Page 95: ...distant time intervals Fluorescence signal may significantly decrease over a longer period of time especially when using low volumes Depending on the amount of evaporation the meniscus will shift to a...

Page 96: ...tion microplate wells separate from background as far as the background noise level allows for that Note If any well of interest is assigned OVER overflow you may manually reduce the gain or select an...

Page 97: ...olved Fluorescence For TRF signal integration parameters need to be adjusted according to the label The start of the signal Integration Time is delayed against the preceding flash by a Lag Time TRF ti...

Page 98: ...nfinite M200 can switch between two wavelengths within 150 ms in case that the selected labels are measured with the same gain and no order sorting OS switching point is involved see Table 6 3 1 for s...

Page 99: ...elengths We do not recommend using volumes less than a third of the maximum volume When using lower volumes check the availability of a suitable plate type In order to ensure good performance for Fluo...

Page 100: ...784 209 Greiner Bio One www gbo com bioscience GRE384st 784 201 Greiner Bio One www gbo com biosciencer GRE384sw 784 207 Greiner Bio One www gbo com bioscience COS6ft 3506 3516 Corning www corning com...

Page 101: ...g www corning com lifesciences COS384fb_low volume 3676 NBS 3677 Med Binding Corning www corning com lifesciences COS384fw_low volume 3673 NBS 3674 Med Binding Corning www corning com lifesciences NUN...

Page 102: ...vely Polarization is a dimensionless unit generally expressed in mP units To start an FP measurement the program strip must contain a valid measurement Blank range and valid G Factor settings 6 4 2 Me...

Page 103: ...avelength of the fluorophore by measuring a sample with a known polarization value A valid calibration of the instrument resulting in a G factor is an important requirement for each fluorescence polar...

Page 104: ...blank Note By filling in more than one well with polarization references and reference blanks the mean values will be calculated and therefore the calibration result will be more accurate Translation...

Page 105: ...Factor recognition 6 4 6 Measurement with a Calibrated G Factor Note Once calibrated the G factor is shown and can be used immediately if it matches the Ex Em wavelength pair and the filter slide desc...

Page 106: ...tch the calibrated value e g the G Factor has been manually changed or loaded with a method the corresponding value will be marked as Manual G Factor The calibrated G Factor can be restored by clickin...

Page 107: ...lank Reduction The mean value of the respective blank wells is subtracted from each value well each for RFU RFU RFU RFU RFU RFU RFU RFU RFU par blk par blk par blk par smp par buf par buf par buf par...

Page 108: ...d This can give rise to fluctuations of the measured values The effect has been observed in wells of 96 well plates and larger wells 6 5 2 Absorbance Ratio Mode Ratio Mode Using the Standard tab in i...

Page 109: ...gram strip by selecting the Multiple reads per well check box see Figure 6 1 below Figure 6 1 Multiple reads per Well Note The function multiple reads per well is only available for the fixed waveleng...

Page 110: ...ble 6 6 1 Theoretically calculated beam diameter at the focal point The MRW type displayed in the software is therefore only a schematic overview of the measurement pattern When measuring real samples...

Page 111: ...ght beam and wall of the microplate well STOP Caution All absorbance and fluorescence intensity specifications given in this document are only valid for single point measurements one measurement point...

Page 112: ...e OD or RFU see Figure 6 3 for result of a fluorescence measurement In addition the standard deviation Stdev and the average value Mean of the measurements points well are also displayed Figure 6 2 Al...

Page 113: ...cond are proportional to the light intensity Increase of measurement time per well yields more accurate values because of the irregular photon impact photon statistics The photonic noise shot noise ca...

Page 114: ......

Page 115: ...See 7 3 Fluorescence Intensity and Time Resolved Fluorescence TRF Fluorescence Polarization See 7 4 Fluorescence Polarization FP infinite F200 only Absorbance See 7 5 Absorbance Glow Type Luminescence...

Page 116: ...08 flashes Optics Fused Silica Lenses Fluorescence Detector Standard PMT Spectrally enhanced red sensitive PMT Luminescence Detector Low dark count photomultiplier tube photon counting electronics Bas...

Page 117: ...ndard Filter Not applicable instruments are equipped with user defined filters Parameters Instrument type Characteristics Wavelength Range standard Excitation 230 600 nm spectrally enhanced Excitation...

Page 118: ...luorescein Fluorescence Intensity Top Plate Type number of wells 96 Dispensed Volume l 200 Flashes Reads per Well 25 Fluorescein Detection Limit pM 20 pM Uniformity at 25 nM Fluorescein 3 CV Reproduci...

Page 119: ...anced Excitation 300 850 nm standard Emission 330 600 nm spectrally enhanced Emission 330 850 nm Standard Filter both Configuration of Default filter slide Exc 485 20 nm Position on Exc filter slide E...

Page 120: ...1 nm steps possible Measurement Range Both 0 3 OD The following specifications are valid for the wavelength range from 300 700 nm for infinite 200 series Plate type number of wells 96 Accuracy 0 2 OD...

Page 121: ...eristics Wavelength Range 380 600 nm Lin Dynamic Range 6 orders of magnitude 7 orders of magnitude extended dynamic range Integration Time well 1 20000 ms Cross Talk black plate 0 01 Measurement range...

Page 122: ...ions for the measurement Luminescence Detection uses the photon counting technique Parameters Characteristics Wavelength Range 380 600 nm Measurement Range 6 orders of magnitude Integration Time well...

Page 123: ...ristics Built in Wavelength See 5 7 Luminescence System Integration Time 1 20000 ms Different integration times are possible for each wavelength Plate Type 96 and 384 well microplates Dynamic Range 6...

Page 124: ...selection in 1 nm steps possible Measurement Range 0 3 OD The following specifications are valid for the wavelength range from 300 700 nm number of flashes 25 Cuvette types 12 5 x 12 5 mm maximum heig...

Page 125: ...7581 Rev No 1 4 125 7 11 Injector Parameters Characteristics Accuracy 10 for injection volume of 10 l 2 for injection volume of 100 l 0 7 for injection volume of 450 l Precision 10 for injection volum...

Page 126: ......

Page 127: ...e instructions carefully The user should determine the appropriate intervals for this testing based on how frequently the instrument is operated We recommend adapting these tests and the acceptance cr...

Page 128: ...Sensitivity 20 pM Fluorescein Fluorescence Top Uniformity 3 CV Fluorescence Top Precision 2 CV Fluorescence Bottom Sensitivity 100 pM Fluorescein Fluorescence Bottom Uniformity 3 CV Fluorescence Bott...

Page 129: ...n These test instructions are valid for the infinite 200 infinite F200 infinite M200 Standard version Spectrally enhanced version Sensitivity Perform the following measurement to determine the detecti...

Page 130: ...rial Reagents 1 nm Fluorescein in 0 01 M NaOH Fluorescein sodium salt Sigma F6377 0 01 M NaOH Blank NaOH pellets Merck article no 6495 or Sigma S8045 1 Greiner 96 well plate black 200 l Pipette tips C...

Page 131: ...lution 0 01 M NaOH into the appropriate wells according to the plate layout 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G H 1 nm Fluorescein Blank 1 nm Fluorescein Blank 1 nm Fluorescein Blank 1 nm Fluores...

Page 132: ...Fluorescein or the blank solution 0 01 M NaOH into the appropriate wells according to the plate layout 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G H 1 nm Fluorescein Blank 1 nm Fluorescein Blank 1 nm Fl...

Page 133: ...ly enhanced version Sensitivity Perform the following measurement to determine the detection limit for Fluorescein Measurement Parameters Parameter Setting Reading Mode Fluorescence Bottom Exc Wavelen...

Page 134: ...escence Bottom Exc Wavelength infinite F200 485 20 nm infinite M200 485 nm Em Wavelength infinite F200 535 25 nm infinite M200 535 nm Number of flashes 25 Integration Time 40 Settle Time 0 Gain Optima...

Page 135: ...Time 0 Gain Optimal Plate Type GRE96fb Part of the Plate A1 Kinetic 20 Cycles Interval Time Minimal Plate Layout 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G H 25 nm Fluorescein Blank 25 nm Fluorescein B...

Page 136: ...tivity Perform the following measurement to determine the sensitivity Measurement Parameters Parameter Setting Reading Mode Fluorescence Top Exc Wavelength infinite F200 340 nm 35 infinite M200 340 nm...

Page 137: ...following measurement to determine the precision reproducibility Measurement Parameters Parameter Setting Reading Mode Fluorescence Top Exc Wavelength infinite F200 340 nm 35 infinite M200 340 nm Em W...

Page 138: ...s are valid for the infinite F200 Standard version Spectrally enhanced version Precision Perform the following measurement to determine the precision Measurement Parameters Parameter Setting Reading M...

Page 139: ...ank 1 nm Fluorescein Blank Filling volume 200 l well Material Reagents 1 nm Fluorescein in 0 01 M NaOH Fluorescein sodium salt Sigma F6377 0 01 M NaOH Blank NaOH pellets Merck Article No 6495 or Sigma...

Page 140: ...nescence Integration Time 1000 ms Settle Time 0 Plate Type GRE96fw Part of the Plate A1 D10 Plate Layout Pipette 200 l of the ATP reagents into the appropriate wells according to the plate layout 1 2...

Page 141: ...ctionL 2 10 8 Concentration of ATP standard M StdevB Standard deviation of Blank meanATP Average of wells filled with ATP standard meanB Average of Blank wells 0 0002 Conversion into mol well 1 1e 15...

Page 142: ...y valid for the infinite M200 Measurement Parameters Parameter Setting Reading Mode Absorbance Scan Measurement Wavelength from to 300 850 nm Step Size 1 nm Number of flashes 25 Settle Time 0 Plate Ty...

Page 143: ...rement Parameters Parameter Setting Reading Mode Absorbance Measurement Wavelength from to 300 700 nm Number of flashes 25 Settle Time 0 Plate Type GRE96ft Part of the Plate A1 Plate Layout No plate i...

Page 144: ...Reading Mode Absorbance Measurement Wavelength 340 nm 405 nm 492 nm 590 nm 620 nm 700 nm Number of flashes 25 Settle Time 0 Plate Type GRE96ft Part of the Plate A1 Kinetic Cycles 20 Minimal Interval T...

Page 145: ...determine the absorbance accuracy Measurement Parameters Parameter Setting Reading Mode Absorbance Measurement Wavelength 1 440 nm 2 635 nm Number of flashes 25 Time between Move and Read 0 Cuvette T...

Page 146: ......

Page 147: ...o prolong the instrument s life and to reduce the need for servicing This section contains the following procedures Liquid Spills Instrument Disinfection Disinfection Certificate Instrument and Materi...

Page 148: ...ILLS MUST BE TREATED AS POTENTIALLY INFECTIOUS THEREFORE ALWAYS ADHERE TO APPLICABLE SAFETY PRECAUTIONS INCLUDING THE WEARING OF POWDER FREE GLOVES SAFETY GLASSES AND PROTECTIVE CLOTHING TO AVOID POTE...

Page 149: ...BE TREATED AS POTENTIALLY INFECTIOUS AREAS IT IS ADVISABLE TO ADHERE TO APPLICABLE SAFETY PRECAUTIONS INCLUDING THE WEARING OF POWDER FREE GLOVES SAFETY GLASSES AND PROTECTIVE CLOTHING TO AVOID POTENT...

Page 150: ...ument from the main power supply 2 Disconnect the instrument from any accessories that are used 3 Carefully wipe all outside surfaces of the instrument with a wad of cotton wool soaked in the disinfec...

Page 151: ...ction procedure must be performed in a well ventilated room by authorized and trained personnel wearing disposable powder free gloves protective glasses and protective clothing The disinfection proced...

Page 152: ...of waste material accumulating in connection with the instrument STOP Caution Observe all federal state and local environmental regulations ATTENTION DIRECTIVE 2002 96 EC ON WASTE ELECTRICAL AND ELEC...

Page 153: ...r future use e g for transport and storage purposes return the packaging of the product spare parts and options via the field service engineer to the manufacturer 9 5 2 Disposal of Operating Material...

Page 154: ...t STOP Caution Always disinfect the instrument before disposal Pollution degree 2 IEC EN 61010 1 Method of disposal Contaminated waste WARNING DEPENDING ON THE APPLICATIONS PARTS OF THE HYDROFLEX PLAT...

Page 155: ...n customer support office 8 Invalid module number Nr Unspecific error in the Instrument Computer communication protocol Please report this error to your local Tecan customer support office 9 Binary Tr...

Page 156: ...nal communication error Please report this error to your local Tecan customer support office 28 Plunger Move not allowed Unspecific internal communication error Please report this error to your local...

Page 157: ...is error to your local customer support office 43 Prepare channel Wavelength lambda Gain g Counts cts Unspecific Hardware failure Please report this error to your local Tecan customer support office 4...

Page 158: ......

Page 159: ...ct 96 Disinfection 151 Dispense Mode 40 Disposal Instrument 154 Operating Material 155 Packing Material 155 E Emission Filter 76 Excitation 75 Excitation Spot Size 71 F Filter Switch Time 100 Filter w...

Page 160: ...tte Port 80 Optical System Fluorescence Bottom 68 Optical System Fluorescence Top 67 Optical System Luminescence 91 OVER 98 overflow 98 P Packing Material Disposal 155 Returning 155 PMT Properties 98...

Page 161: ...ECAN DE F 49 7951 9417 92 helpdesk de tecan com Tecan France S A 26 avenue Tony Garnier F 69007 Lyon France T 33 820 88 77 36 F 33 4 72 76 04 99 helpdesk fr tecan com Tecan Ib rica Spain Sabino de Ara...

Page 162: ...8 953 3101 Toll Free in the US T 1 866 798 3226 helpdesk sy tecan com Tecan UK Theale Court 11 13 High Street Theale UK Reading RG7 5AH United Kingdom T 44 118 930 0300 F 44 118 930 5671 helpdesk uk t...

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