2. General Description
46
Instructions for Use for
infinite® 200
No. 30017581 Rev. No. 1.4
2008-07
Example 4: Measurement of Ca
2+
sensitive probes – Indo-1
Plate
Select an appropriate plate type. For fluorescence
measurements, black microplates are recommended.
For this example, a black 96 well plate was selected.
Part of the plate
(Not shown); can be optionally selected if only part of the
plate should be processed
Well
Mandatory for measurements with ‘injection’
Kinetic Cycle
Select the number of necessary cycles
Kinetic condition
This strip allows actions to be performed once in a kinetic
run at a certain cycle. The intended injection strip below it
is only processed once at the selected cycle.
Injection
Injector A injects 20 µl with speed 200 µl/sec., refill mode:
not selectable; injection is performed at cycle 5 (defined
by kinetic condition strip)
Fluorescence Intensity (1)
Select the appropriate parameters for the first label:
Excitation wavelength: 340 nm, Emission wavelength:
410 nm; number of flashes: 25; integration time: 40; gain:
manual
Fluorescence intensity (2)
Select the appropriate parameters for the second label:
Excitation wavelength: 340 nm, Emission wavelength:
480 nm; number of flashes: 25; integration time: 40; gain:
manual
Move Plate
Plate is moved out after finishing all wells
