Prism6 & Crystal Reader software User Manual
5
3. Intended use of the naica® system
The naica
®
system for Crystal Digital PCR
TM
is composed of two instruments: the Geode, which
performs droplet generation and amplification, and the Prism6, which enables imaging of the
droplet crystals in up to 6 detection channels. The Crystal Reader software is used to control and
set up Crystal Digital PCR™ experiments on the Prism6 instrument.
The Crystal Reader software functions as the user interface to set up the experiment for the
Prism6 and is dedicated to the image acquisition in the Crystal Digital
PCR™ workflow.
The Crystal Reader software:
o
Allows defining the analytical context of the experiments. Experiments can be set up
on-demand or dedicated experimental templates can be created for recurring
experimental setups.
o
Controls the Prism6 instrument for the acquisition of the fluorescence images of
Sapphire or Opal chips.
o
Applies pre-analysis treatments to the acquired images and provides a first quality
control in preparation for the detailed experiment analysis performed by the Crystal
Miner software.
The Crystal Miner software is used to extract data from the images acquired using the Prism6
and to calculate the absolute concentrations of the targeted nucleic acids. The software is pre-
installed on the Prism6 delivered as part of the naica® system.
The naica® system performs Crystal Digital PCR™ within microfluidic chips (Sapphire chips &
Opal chips). The naica® PCR MIX reagents are recommended for use to achieve optimal Crystal
Digital PCR™ performance on the naica® system.
For detailed instructions for the Geode and the Crystal Miner software, please refer to the
respective User Manual. For detailed instructions for Sapphire chips, Opal chips, naica® PCR
MIX, and naica® multiplex PCR MIX refer to the respective Instruction for Use (IFU)
.
The naica
®
system is a laboratory instrument to be used by qualified personnel in a controlled
environment. Before using the naica
®
system, the user should be trained by a Stilla Technologies
representative.
In general, Crystal Digital PCR™ can be performed with all types of DNA sample types on the
naica® system. However, individual sample-type compatibility for digital PCR applications may
require a dedicated assay validation by the end-user. Please note that the extraction method
used and sample purity might influence sample compatibility for digital PCR applications as well.
For application-specific references, please visit the website.
The Prism6 instrument is intended for use by professional users trained in molecular biological
techniques and the operation of the Prism6 instrument.
The Prism6 instrument is part of the naica® system. The naica
®
system is intended for Research
Use Only. Not for use in diagnostic procedures.
