Prism6 & Crystal Reader software User Manual
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The “Comments” field can be used to add
any information pertaining to the experimental
record.
Figure 11
: Enter fluorophore and target names in the “Experiment Details” interface.
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Go to the “Experiment Details” button in the left panel, then enter for each detection
channel (“Blue”, “Teal”, Green”, “Yellow” “Red” and “Infra-Red” LED):
•
The name of the fluorophore that is mainly excited by the LED.
•
The name of the target associated with this fluorophore.
•
Select/unselect the checkbox to include/exclude a detection channel from
scanning.
•
Selecting a minimum of three detection channels, including blue are mandatory (it
is the main detection channel).
Note: Ensure to select the appropriate detection channel for the experiment to avoid any false-
positive results. In case of selection of a wrong detection channel, re-scan the chips.
o
Go to
“Embedded Files” selection menu and select the desired:
The following files are (or can be) embedded into the experiment file:
o
The Image Analysis Configuration File (mandatory)
o
The Spillover Compensation Matrix (optional)
o
The Plots configurations (optional)
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The Analysis Configuration (optional)
Edit experiment details: