Prism6 & Crystal Reader software User Manual
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Figure 22: Zoom-in into a chamber image in the Blue detection channel (all negative droplets
should be visible in the Blue channel image) in the Crystal Reader software.
o
The Grayscale Histogram tool (bottom right) might help you to appreciate the distribution
of pixel intensities expressed in RFU (Relative Fluorescence Unit), where 0 RFU indicates
black and 2
16
– 1 = 65535 RFU indicates saturation.
Figure 23: Step (5)- Use the histogram to place intensity cursors and reveals the pixels
corresponding to the RFU interval in the Crystal Reader software.
