Prism6 & Crystal Reader software User Manual
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corrected by checking that the chip holder is clean and the chamber is well
placed).
▪
“Number of analyzable droplets”: the higher the number the better the
confidence in predicted concentration results.
Note: Saturated droplets and droplets covered by artifacts are not
analyzable. They can be avoided by checking that exposure times are not
too high, and the chamber is cleaned up
.
▪
“Number of saturated objects”: the lower the number the better the
chamber quality.
Note: Counted saturated objects are either saturated droplets caused by
a high exposure time or saturated artifacts located inside the chamber.
They can be avoided by checking that exposure times are not too high,
and the chamber is cleaned up.
If exposure times need to be modified to improve the image quality, the chips should be
rescanned (see the section “Advanced Functionalities” for more details about chamber
rescan).
If some of the chambers need to be cleaned to improve the image quality, the chips
should be rescanned for the respective chambers (see the section “Advanced
Functionalities” for more details about chamber rescan).
In case of artifacts, such as dust (yellow flag for the number of saturated objects not due
to saturation of droplets), clean the back surface of the chip by slowly sliding it 1 time on
the length directly on a cleaning wipe (delivered with the respective chips) and rescan.
The saved experiment can be now analyzed with Crystal Miner software (please refer to
Crystal Miner software User Manual available on the Technical Resources webpage for
details).
o
Before turning the Prism6 instrument off, c
lick on “Open tray”/“Close tray” to remove the
chips. Proceed by pressing on the power button located in the front (the green light
becomes orange) for a few seconds. .
V. Advanced Functionalities
1. How to rescan existing chambers? How to scan new ones?
o
Click on the RESCAN button
One of 3 options can be chosen from:
•
a) Rescan all the chambers of the experiment.
•
b) Scan some new chambers or chips and keep the images of the already
scanned chambers.
•
c) Rescan only specific chambers of the experiment and keep the images of the
other chambers.
Note: If different scanning parameters or experimental details are to be applied to the same
set of samples for an experiment, the chips can be scanned up to three times within 48 hours
following the first scan.
