Prism6 & Crystal Reader software User Manual
55
For Opal chips
Observation
Possible cause
Recommendation
A low average number of droplets partitioned
During the loading of the chips, multiple
droplets could have been generated in the
inlet port.
Those droplets were probably not merged
properly using the electrostatic pen.
Make sure that the electrostatic pen is working.
For more information about how to use the
electrostatic pen with the opal chips, please refer
to the IFU for Opal chips.
The Geode is not tilted enough (front-back
axis) or is tilted in the wrong direction.
Contact Technical Support if unsure about the
correct positioning of the Geode.
The small and defined population of droplets with different
sizes.
(The population is often positioned close to the chip injectors, and it
is composed of droplets often smaller than the normal ones)
The normal cycling of temperature and
pressure could cause, in some rare cases, the
presence of these artifacts.
This population of droplets is excluded during
the analysis. The software recognizes the
different sizes of the droplets and considers
them as artifacts. This phenomenon usually
does not affect the output of the assay.
