Prism6 & Crystal Reader software User Manual
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For the dilution factor, in case no value is specifically added, the final concentration in copies/µL
will correspond to the number of DNA copies for each µL in the whole 25 µL of reaction mix for
Sapphire chips and 7 µL of reaction mix for Opal chips.
If, for example, the volume of DNA solution is 4 µL out of 25 µL of reaction mix, it could be useful
to insert a dilution factor of 6.25 (25 µL/4 µL) to obtain the effective concentration of copies/µL in
the 4 µL of the initial sample. To unlink the dilution values among the channels, click on
the “link
icon”
.
Note: It is possible to navigate between the chambers using the arrows of the keyboard while
pressing the “ALT” key. This method may help to save some time during the edition of the
chamber context.
The chamber details are displayed on the chamber button of the Tray Holder:
The sample ID and sample types per channel are displayed on the chamber button.
Note: Before the scan is done, the flag button is grayed in deactivated. The user will be able to
click on this button once the chamber has been scanned and analyzed
.
3. Step (4) - Launch the scan:
o
Click on the “SCAN” button, then validate the path to save scanned data (as a “.ncx” file)
and confirm the loading of the first chip holder to launch the scan.
Figure 17: Pop-up confirmation before launching the scan.
If the chips to be scanned are loaded in the scanner, click on “OK”, else click on
“CANCEL” and go back to the previous steps.
Once the scan is processing, a progress bar with the estimated remaining time is
displayed.
CAUTION!
Do not push the power button on the front panel of the Prism6 instrument while it is still
in operation. This would cause a shutdown of the instrument and displays a risk of data
being lost and a consequent requirement to re-scan the chips.
