RADIOMETER ABL800 FLEX, Reference Manual

Page: 132 / 206

5. Performance characteristics ABL800 FLEX Reference Manual
Interference tests,
Continued
The substances against which the oximetry parameters ( c tHb, s O
2
, F O
2
Hb,
F COHb, F MetHb, F HHb, F HbF) and c tBil were tested for interference are given
in the table below:
Oximetry
parameters
(SAT100 blood reference test sample: c tHb=15 g/dL, s O
2
=100 %, F COHb=0.7 %,
F MetHb=0.5 %, c tBil=0, pH=7.4. Parameter sensitivity from the influence on the
absorbance spectrum from various substances.)
Change on …
Substance Test conc. ctHb
(g/dL)
sO
2
(%)
FO
2
Hb
(%)
FCOHb
(%)
FMetHb
(%)
FHHb
(%)
FHbF
(%)
ctBil
(
µ
mol/L)
Intralipid 4 Vol %
e)
−
0.5
0.1
−
1.3
0.5 0.9
−
0.1
11 0
4
b)
Intralipid 2 Vol %
f) −
0.4
0.1
−
0.3
0.3 0.1
−
0.1
11 7
2
b)
HbF
a), c)
20 %
−
0.02
1.17 0.04 0.73 0.37
−
1.14
0
−
14
S Hb 10 % 0
−
1.0
0.9
−
0.1
0.1
−
0.9
Not tested
pH 7.1
7.9
−
0.5
−
0.6
−
0.5
0.6
−
0.2
−
0.5
−
0.4
1.0
0.1
0.1 0.5
−
0.6
−
19
13
0
−
5
Cardio Green
c)
5 mg/L
−
0.16
0.29 1.14 0.07
−
0.93
−
0.29
−
5
−
20
Evans Blue
c)
5 mg/L
−
0.04
0.14 0.28
−
0.20
−
0.20
0.14
−
5
5
Betacarotene in
plasma
c)
3.7
µ
mol/L
0.0
−
0.02
0.03
−
0.01
−
0.04
0.02 0.1
−
0.2
Patent Blue V
c)
10 mg/L
−
0.16
0.39 0.86
−
0.47
0.00
−
0.38
−
21
38
Methylene Blue
c)
30 mg/L
−
0.7
−
3.4
5.6
−
3.0
−
6.2
3.6
−
37
−
25
HiCN
c)
0.11
mmol/L
0.26
−
1.5
−
3.0
−
0.5
0.5 1.5 24 47
MCHC
c), d)
newborn range
320 g/L
350 g/L No interference
−
12
17
Sedimentation rate 100 arb.
Units
≤ ±
0.5
No interference Not
tested
Notes:
a) If function “Correction for HbF levels less than 20 %” is activated, the change is 0 for all
parameters.
b) Plasma sample.
c) Calculated value from mathematical superposition of measured pure interference spectrum
on measured reference spectrum.
d)
c tBil = 400
µ
mol/L.
e) Intralipid (20 % solution) at 4 Vol % gives final test level of 0.8 %.
f) Intralipid (20 % solution) at 2 Vol % gives final test level of 0.4 %.
There is no interference from fetal hemoglobin (HbF) when the analyzer applies HbF correction.
There is no interference from bilirubin (conjugated/unconjugated) up to 1000
µ
mol/L.
Continued on next page
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