QIAcube Connect MDx User Manual 12/2020
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If for any reason the robotic arm prevents you from reaching a loading position, do not move the
robotic arm manually. Instead, proceed as follows:
Press
Move left
. The robotic arm will start to move. The hood can remain open during this
movement.
Ensure that you stand clear of the instrument while the robotic arm is moving. Wait until the
robotic arm has completed its movements.
Follow the instructions below to load enzyme, reagents and tips:
1.
Prepare enzymes and/or reagent(s) listed on the screen. Refer to the corresponding kit
handbook for more information. Before proceeding to the next step, make sure that you read
all essential and critical information indicated in the
Information
icon ( ) marked in red.
2.
Make sure that you are using the correct tube type. Press the
Information
icon ( ) in the
respective row to show the details.
Supported enzyme tube types are 1.5 ml micro-centrifuge tube (Sarstedt®, Cat. no.: 72.706),
2 ml screw-cap tube without skirted base (QIAGEN, Cat. no.: 990382) and 2 ml Processing
tube (QIAGEN, supplied with PAXgene Blood RNA Kit).
3.
Make sure that you provide the correct volume as indicated on screen. The volume shown on
the screen is the minimum volume to be loaded. To avoid spill-over during the run, do not
exceed this volume significantly.
4.
Place the opened tube in the worktable position as indicated in the table on the screen. It is
important that you load the tube in the correct worktable position.
5.
Place the lid of the microcentrifuge tube securely in the lid slot next to the tube.
6.
Make sure that the required number of tips for each tip type are loaded as indicated on the
screen. It is possible to use up the tip racks, if the minimum required number of tips for each
type is loaded. However, it is recommended to load more than the minimum amount of tips.
The loading position shown on the screen is the recommended position for tip racks. The
position can also be changed. Later, when starting the run, the instrument will check if the
correct tip racks are placed on the worktable and if there are enough tips for the protocol run.
There are 3 different kinds of tip racks that can be used on the QIAcube Connect MDx,
depending on the protocol selected. A blue rack for 200 µl filter-tips, a light grey rack for
1000 µl filter-tips and a dark grey rack for 1000 µl wide-bore filter-tips. The instrument uses
the notches on the filter-tip rack to identify the type. To prevent mix up that may lead to a run
issue,
do not manually refill tip racks
. Only use tips designed for use with the QIAcube
Connect MDx.
Important
: Do not use damaged filter tips. Do not load damaged tip racks onto the worktable.
