23
5.6
Respiration Vials
Based on the contactless sensor
technology from
Pyro Science
, ready
assembled
respiration
vials
with
integrated
oxygen
sensors
are
available with 4ml or 20ml volume
(item no.
OXVIAL4
and
OXVIAL20
),
respectively. Stripes of the oxygen sensitive
REDFLASH indicator
are glued to the inner wall of the respiration vials. Separately
available adapter rings (item no.
ADVIAL4
and
ADVIAL20
) allow
an easy fixation of a bare optical fiber (item no.
SPFIB-BARE
or
SPFIB-BARE-CL2
), which connects the respiration vial to one
channel of the
FireStingO2
. The bare-fiber-versions of the optical
fiber allow submersion in a water bath, as their fiber ends do not
contain metallic parts potentially prone to corrosion. The position
of the adapter rings (and the spot fiber) can be fixed by clamping
screws. The elongated shape of the built-in sensor allows
oxygen measurements at different heights within the vial by
simply sliding the adapter rings up and down. Therefore it is e.g.
possible to measure the oxygen content both in a liquid and in
the gaseous headspace within the same vial.
For high precision respiration rate measurements, the respiration
vials would be typically placed within a water bath with a regulated
constant temperature at
0.1°C precision. If higher temperature
fluctuations are expected, the oxygen measurement should be
automatically
temperature
compensated
by
connecting
a temperature sensor (item no.
TDIP15
or
TSUB36
) to the
FireStingO2
(see chapter 10) and placing it in the water bath.
The respiration vials can be used for small-scale respirometry and
metabolic rate measurements of e.g. cell cultures, eggs, larvae,
small crustaceans, small fish, water-, plant-, algal-samples etc.
