Promega GloMax GM2000 Operating Manual Download Page 59

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Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516

TM470 · 4/16

www.promega.com

Reporter Assays

Product

Size Cat.#

Dual-Glo

Luciferase Assay System*

10ml

E2920

Dual-Luciferase

Luciferase Reporter Assay System*

100 assays

E1910

Luciferase Assay System*

100 assays

E1500

NanoBiT™ PPI MCS Starter System

1 each

N2014

NanoBiT™ PPI Flexi

Starter System

1 each

N2015

Nano-Glo

Live Cell Assay System*

100 assays

N2011

Nano-Glo

Luciferase Assay System*

100ml

N1120

Nano-Glo

Dual-Luciferase

Reporter Assay System*

100ml

N1620

ONE-Glo™ Luciferase Assay System*

10ml

E6110

Bright-Glo

™

Luciferase Assay System*

10ml

E2610

Steady-Glo

Luciferase Assay System*

10ml

E2510

Renilla

Luciferase Assay System*

100 assays

E2810

Renilla

-Glo

Luciferase Assay System*

10ml

E2710

ADCC Reporter Bioassay, Core Kit

1 each

G7010

ADCC Reporter Bioassay, Core Kit 5X

1 each

G7018

ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay*

1 plate

E7110

*Additional Sizes Available

Cell Signaling Assays

Product

Size Cat.#

P450-Glo™ CYP1A2 Screening System

1,000 assays

V9770

P450-Glo™ CYP2C9 Screening System

1,000 assays

V9790

P450-Glo™ CYP3A4 Screening System

1,000 assays

V9800

P450-Glo™ CYP2C19 Screening System

1,000 assays

V9880

P450-Glo™ CYP2D6 Screening System

1,000 assays

V9890

ADP-Glo™ Kinase Assay*

1,000 assays

V9101

Kinase-Glo

Luminescent Kinase Assay*

10ml

V6711

Kinase-Glo

Max Luminescent Kinase Assay*

10ml

V6071

Kinase-Glo

Plus Luminescent Kinase Assay*

10ml

V3771

cAMP-Glo™ Assay*

300 assays (384-well plate)

V1501

GloSensor™ cAMP Reagent*

25mg

E1290

*Additional Sizes Available.

Summary of Contents for GloMax GM2000

Page 1: ...4 16 TM470 GloMax Navigator System Instructions for Use of Products GM2000 and GM2010 O P E R A T I N G M A N U A L...

Page 2: ...ng Up the GloMax Navigator Instrument and Tablet PC 10 2 B Switching On the GloMax Navigator Instrument 11 2 C Shutting Down the GloMax Navigator Instrument 12 2 D Operating the GloMax Navigator Syste...

Page 3: ...glow and flash based luminescent reactions in 96 well plates The instrument is operated by an integrated Tablet PC which provides quick and easy navigation through the control options Exporting your...

Page 4: ...ately 40lb 18 1kg Power Requirements 0 5A 100 240V AC 50 60Hz universal Warranty 1 year parts and labor warranty included Regulatory Compliance For Research Use Only Not for use in Diagnostic Procedur...

Page 5: ...of luciferase Dynamic Range 9 logs Cross Talk Less than 3 10 5 white 96 well plate Corning Cat 3912 Injector System Specifications Number of Injectors optional Two injectors Dispense Volume Range 5 20...

Page 6: ...gator has already been configured for the settings listed above 1 C Product Components P R O D U C T S I Z E C AT GloMax Navigator System 1 each GM2000 For Research Use Not for use in Diagnostic Proce...

Page 7: ...to the equipment or surrounding area Avertissement Risque de pr judice corporel pour l op rateur ou d accident avec l instrument ou l entourage 6687TA eps It is important to understand and follow all...

Page 8: ...void the user s authority to operate the equipment Do not use this device in proximity to sources of strong electromagnetic radiation e g microwave oven because they may interfere with the proper oper...

Page 9: ...x Navigator n est pas en cours d utilisation 5295MA eps The GloMax Navigator is intended for indoor use only Le GloMax Navigator est destin une utilisation en int rieur 5295MA eps Always disconnect th...

Page 10: ...vice If any item is damaged contact Promega Technical Services E mail techserv promega com Standard components are shown in Figure 2 Panel A below Figure 2 GloMax Navigator System Accessory Components...

Page 11: ...nt and Tablet PC 1 Unpack the Tablet PC from its shipping box 2 Place the Tablet PC in the Tablet Holder on the top of the GloMax Navigator Instrument in the orientation shown in Figure 4 Fi 13516TA g...

Page 12: ...ure 6 The back of the GloMax Navigator Instrument A On Off switch B Power connection C USB port to connect to Tablet PC D Two USB ports 2 B Switching On the GloMax Navigator Instrument 1 Switch the in...

Page 13: ...or Software by tapping on the x in the upper right corner Figure 7 3 Shut down the Tablet PC using normal Windows 8 shutdown procedures Swipe in from the right edge of the screen select Settings then...

Page 14: ...ng inside the text box and entering a name Figure 9 13596TA Figure 9 Save Results Window 6 Select Start to begin the protocol 7 The protocol will begin acquiring data and will display the results in t...

Page 15: ...button will provide you a list of all available protocols These include preloaded protocols as well as user defined protocols The user can create user defined protocols from this screen as well Smart...

Page 16: ...ectors to Prime Reverse Purge and or Flush and accessing the instrument information About Eject Tapping this button will move the Plate Tray to the Home position to add or remove a plate from the inst...

Page 17: ...g All Favorite protocols can be marked by tapping the Star adjacent to the protocol name After marking a protocol as a favorite those protocols will automatically be listed at the top of the Protocol...

Page 18: ...trument 3 Tap OK when ready to begin the protocol The protocol will begin when OK is selected so ensure the plate has been loaded into the instrument 4 When the read is complete the user will be promp...

Page 19: ...New Protocol button at the bottom of the screen Figure 12 2 You will be prompted to name the protocol You also may provide a description if desired 11630TA Figure 15 New Protocol name prompt from the...

Page 20: ...n the bottom right corner of the screen A user message will appear indicating that the protocol was saved successfully F 11633TA igure 18 GloMax Navigator New Protocol Successfully Saved user message...

Page 21: ...ified whereas the plate maps within each step of the protocol are now active and the user can modify each step as desired Figure 13605TA Plate Map is unlocked and cannot be applied Select Plate Map fo...

Page 22: ...tocol Window The functions can be expanded and contracted by selecting the black triangle at the top left corner of each function They may be removed by selecting the black X at the top right corner o...

Page 23: ...200 l and the injection speed 20 l sec to 500 l sec Select the wells that will be injected by touching the plate map and selecting the desired wells Warning Make sure sufficient space is available in...

Page 24: ...is checked the Prompt screen shown in Figure 25 appears to the user during protocol with the option to open the door If Eject Plate is selected the instrument will home and the user can then open the...

Page 25: ...um of 100 loops can be performed within a single protocol 1 Add the Loop step to the protocol by dragging and dropping the Loop icon into the protocol window F 13611TA igure 26 Loop function 2 Select...

Page 26: ...ocol Window 13613TA Figure 28 Kinetics function 2 All well associated operations within the kinetic block must have the same well selection The plate maps of these operations are not individually edit...

Page 27: ...the results 13615TA View results within a group by sorting on date time name or user Export or remove 1 or more results Select to remove or export file Figure 30 GloMax Navigator Results Screen 2 To...

Page 28: ...view results Figure 32 GloMax Navigator Results screen with heat map display Kinetic results display a graphical representation of the data during the course of the kinetics read Figure 33 Data are au...

Page 29: ...d to a number of locations including a USB flash drive the Tablet PC C drive or a cloud based storage c Two files are automatically exported a comma separated value file and a Microsoft Excel workshee...

Page 30: ...Excel Workbook Note Colors in the Excel workbook will not reflect those within the software heat map and therefore you will need to place the data into cells of similar shades if you wish to maintain...

Page 31: ...21TA Figure 38 The GloMax Navigator Settings Screen Instrument Info Tapping the Instrument Info button reveals a list that includes the software and firmware versions the instrument name and maintenan...

Page 32: ...r Instrument Injector System Specifications Number of Injectors Two Injectors Dispense Volume Range 5 200 l in 1 l increments Injection Speed 20 500 l second Waste Collection Tray Volume Approximately...

Page 33: ...nt axes itself when either the Door button is selected a Protocol is started or if the instrument power is turned Off On Initialize all axes by selecting the Start button in this screen 13633TA Figure...

Page 34: ...for the instrument F 13624TA igure 44 Settings Administrator screen Export Folder Select the folder location to which the data should be exported Once this is configured the data will be exported to t...

Page 35: ...new protocols with instructions directly from the Promega web site www promega com resources tools detection instruments software promega branded instruments glomax navigator Download the protocol fi...

Page 36: ...e of the screen by Today This Week the past 3 Months the past 6 Months This Year or by All Detailed audit records are automatically generated and stored with the GloMax Navigator software that cannot...

Page 37: ...uires injectors the injectors must first be primed with at least 500 l of the reagent to be injected Priming wets the fluid path and removes air bubbles to ensure delivery of an accurate injection vol...

Page 38: ...he injector tube through the Para lm and into the reagent bottle 4 Ensure the Waste Collection Tray is in position and empty 5 Tap the Start button adjacent to the Prime Injectors information in Figur...

Page 39: ...ents left in the fluid lines will be returned to the reagent container 4 C Flushing the Injectors after Use 1 Remove the sample plate from the instrument 2 Tap the Start button adjacent to the Flush o...

Page 40: ...his may cause damage to the instrument and its electronics Do not use solvents or abrasive cleaners Use a cloth dampened with deionized water to periodically wipe clean the exterior of the GloMax Navi...

Page 41: ...f the instrument Figure 54 Fi 13542TC Optical head Microplate sample tray and cover gure 54 Interior of the GloMax Navigator instrument 3 Loosen the hand screws and remove the Injector Tip Holder from...

Page 42: ...to air dry completely before proceeding Alternatively blot dry with a lint free Kimwipes tissue 7 Return the Aperture to the Optical Head Align the Aperture with the Optical Head and gently guide the...

Page 43: ...re to properly maintain a clean interior may cause a mechanical error and the irretrievable loss of data We recommend thoroughly cleaning the interior every 30 days 1 Turn OFF the instrument 2 Use a K...

Page 44: ...nto position 9 Place a container of deionized water on the bottle holder with the inlet tube inserted to reach the bottom of the container Prepare at least 10ml of deionized water for each injector to...

Page 45: ...Tip Holder to the Optical Head and tighten the hand screws Position the holder just above the front pins on the Optical Head Push the holder toward the right to lock the holder securely to the right...

Page 46: ...the instrument has been opened unexpectedly during operation of the instrument Manually opening the door of the instrument during an instrument operation will cause that operation to be aborted Refer...

Page 47: ...tion 4 C Injector speed may not be optimal for the viscosity or composition of the solutions used Injectors not injecting No reagent in injector lines Confirm that the inlet tubing is inserted into th...

Page 48: ...Low activity in sample Use white opaque plates High luminescent cross talk Use high quality white or black opaque plate Injector leaks The inlet or outlet tubing is not properly connected to the inje...

Page 49: ...is 300 l Thoroughly clean the interior of the instrument Reagent has spilled inside the instrument Wipe up spills immediately using an absorbent paper towel or Kimwipes tissue If necessary use a 70 et...

Page 50: ...rogram please ensure it is set to manual not automatic update Antivirus software will slow the performance of the Tablet PC and will affect the performance of the GloMax Navigator Instrument while it...

Page 51: ...ate time zone for your location and whether you wish the clock to automatically adjust for Daylight Savings Time e Tap the OK button to save the time zone settings f You will be returned to the Date a...

Page 52: ...er The Local Users and Groups dialog should appear 2 In the left hand pane of the Computer Management window select Local Users and Groups and double tap Groups Note If the Local Users and Groups menu...

Page 53: ...nt to add users tap PromegaUsers A PromegaUsers window will open which can be used to add existing users to the PromegaUsers group Tap the Add button to add users to the PromegaUsers group In the Ente...

Page 54: ...ting users from the PromegaAdministrators group 4 Select the user you want to remove and tap the Remove button Tap OK to remove selected user from the PromegaAdministrators group Close the Computer Ma...

Page 55: ...ment name from the Instrument Name Window 7 B Icons and Symbols Home screen 11609TA Exit Software 11611TA Quick access to the Tools Screen 13629TA Home all axes 13630TA Help Screen From any screen oth...

Page 56: ...on contact Promega Technical Services Contact information is available at www promega com E mail techserv promega com To obtain service during the warranty period please take the following steps 1 Con...

Page 57: ...ed by toxic carcino genic radioactive or other hazardous materials B I confirm that the returned items have been decontaminated and can be handled without exposing personnel to health hazards Circle t...

Page 58: ...avigator Parts Only Service Agreement 1 each SA1302 GloMax Navigator Basic Service Agreement 1 each SA1303 GloMax Navigator Preventive Maintenance 1 each SA1308 GloMax Navigator Installation Qualifica...

Page 59: ...System 10ml E2510 Renilla Luciferase Assay System 100 assays E2810 Renilla Glo Luciferase Assay System 10ml E2710 ADCC Reporter Bioassay Core Kit 1 each G7010 ADCC Reporter Bioassay Core Kit 5X 1 each...

Page 60: ...NanoBiT ONE Glo P450 Glo and ToxGlo are trademarks of Promega Corporation Windows is a registered trademarks of Microsoft Corporation Kimwipes is a registered trademark of Kimberly Clark Corporation...

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