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Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
TM470 · 4/16
www.promega.com
4.B. Reverse Purge (Retrieving Unused Reagents from Injectors, optional)
1.
Tap the
Start
button adjacent to the Reverse Purge option on the Injectors screen (Figure 50).
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A
Figure 52. Reverse Purge function.
2.
Ensure the Waste Collection Tray is in position and empty.
3.
Tap the
Start Reverse Purge
button (Figure 52).
4.
Any reagents left in the fluid lines will be returned to the reagent container.
4.C. Flushing the Injectors after Use
1.
Remove the sample plate from the instrument.
2.
Tap the
Start
button adjacent to the Flush option on the
Injectors
screen (Figure 50).
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A
Figure 53. Injector Flush function.
3.
Ensure the Waste Collection Tray is in position and empty.
4.
Tap the
Start Flush
button (Figure 53).
The recommended flush protocol uses a sequence of 1 cycle (1ml) of deionized water, 1 cycle (1ml) of 70%
ethanol, 1ml of deionized water and then 1 cycle (1ml) of air to flush the fluid.
Note:
Empty Waste Collection Tray when priming and flushing are complete. See Section 5.F for injector
cleaning instructions.