Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
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www.promega.com
TM470 · 4/16
Symptoms
Causes and Comments
Power failure during run
In the event of a power failure, turn OFF the instrument
and the Tablet PC. When power is resumed, turn the
instrument and Tablet PC back ON. The results that were
generated during the run (before the power failure) will be
saved.
Instrument problem: “door opened”
1. Instrument door was opened during a run,
message: explanation
causing the reading to abort. Restart the run.
2. Something prevented the instrument door from
fully closing. Check the instrument door for
any obstructions.
3. The door sensor may be bent or broken. Contact
Promega Technical Services.
Luminescent signal is reported as SAT
The luminescent output of the sample is saturating the
detector.
1. Use less sample.
2. Use a black plate.
Low luminescent signal
Low activity in sample.
Use white opaque plates.
High luminescent cross talk
Use high-quality white or black opaque plate.
Injector leaks
The inlet or outlet tubing is not properly connected to the
injector pump. See Section 5.H for instructions on
removing and replacing tubing.
Injectors do not sit properly in the
Reagent residue has built up inside the Injector Tip Holder.
Injector Tip Holder
See Section 5.D for instructions on cleaning the Optical
Head.
Grinding noise during run
Reagent residue has built up on the Aperture. See
Section 5.E for instructions on cleaning the interior of the
instrument.
The Microplate Sample Tray cover is open during the run.
Close the tray cover and secure with the tray latch. The
plate holder should be in the Home position.
The Microplate Sample Tray cover does not close
The 96-well plate is not sitting flat in the Microplate
Sample Tray. Gently push the tray cover up to a 90 degree
angle with the Microplate Sample Tray to completely open
the tray. Place the 96-well plate inside the tray.
Remove lid from 96-well plate, place inside the Microplate
Sample Tray and close the tray cover.
