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Sample Preparation
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Microtoming
A microtome is a device for slicing a sample into thin cross-sections, 0.5 to 20 µm
thick. It is commonly used to prepare samples for light microscopy; the same range
of thicknesses is also appropriate for infrared microscopy.
If you are trying to identify the individual components of a laminate, microtomed
samples give the best results.
Samples are often embedded in a supporting medium before they are microtomed.
If you must use an embedding material, choose it carefully, so that it does not alter
the sample by reacting with it, dissolving it, or contaminating it. Some commonly
used materials are:
•
paraffin wax: This is the preferred medium for infrared spectroscopy. It
produces few spectral interferences, and it can usually be easily removed from
the sample with warm xylene.
•
β-pinene wax: This material is similar to paraffin.
•
plastic embedding materials: These can be used depending on the size and
porosity of the sample.
•
acrylic and epoxy resins: Although these are commonly used in light
microscopy, they are not recommended for infrared, because they are hard to
remove and can cause spectral interferences.
Summary of Contents for MultiScope System
Page 1: ...MultiScope System Microscope User s Reference ...
Page 5: ...Introduction ...
Page 14: ...14 MultiScope System Microscope User s Reference ...
Page 15: ...Warnings and Safety Information ...
Page 31: ...Overview of the MultiScope System Microscope ...
Page 44: ...44 MultiScope System Microscope User s Reference ...
Page 45: ...Getting Ready to Use the Microscope ...
Page 53: ...Tutorial Using the Microscope ...
Page 63: ...Preparing Samples ...
Page 83: ...Collecting Spectra with the Microscope ...
Page 95: ...Operating the Optional Equipment ...
Page 109: ...Applications ...
Page 122: ...122 MultiScope System Microscope User s Reference ...
Page 123: ...Maintenance ...